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| Format: | Artículo científico |
| Sprache: | en |
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Molecular ecology resources
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/39903046/ |
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Inhaltsangabe:
- Bamboozle: A Bioinformatic Tool for Identification and Quantification of Intraspecific Barcodes. Pinder, Matthew I M Andersson, Björn Blossom, Hannah Svensson, Marie Rengefors, Karin Töpel, Mats Computational Biology DNA Barcoding, Taxonomic Diatoms Chlamydomonas reinhardtii Polymorphism, Single Nucleotide Evolutionary changes in populations of microbes, such as microalgae, cannot be traced using conventional metabarcoding loci as they lack intraspecific resolution. Consequently, selection and competition processes among strains of the same species cannot be resolved without elaborate isolation, culturing, and genotyping efforts. Bamboozle, a new bioinformatic tool introduced here, scans the entire genome of a species and identifies allele-rich barcodes that enable direct identification of different genetic strains from a population using amplicon sequencing of a single DNA sample. We demonstrate its usefulness by identifying hypervariable barcoding loci (