Preparation of the Hypoallergenic Enzymatic Hydrolyzate of Cra a 4 with the Potential to Induce Immune Tolerance.
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| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of agricultural and food chemistry
2025
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| Materias: | |
| Acceso en línea: | |
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| _version_ | 1868266247431389187 |
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| author | Wu, Ming-Xuan Huan, Fei Gao, Shuai Ni, Ling-Na Gu, Yi Han, Tian-Jiao Liu, Meng Lai, Dong Liu, Qing-Mei Liu, Guang-Ming |
| author_facet | Wu, Ming-Xuan Huan, Fei Gao, Shuai Ni, Ling-Na Gu, Yi Han, Tian-Jiao Liu, Meng Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Wu, Ming-Xuan Huan, Fei Gao, Shuai Ni, Ling-Na Gu, Yi Han, Tian-Jiao Liu, Meng Lai, Dong Liu, Qing-Mei Liu, Guang-Ming |
| collection | PubMed - marine biology |
| contents | Preparation of the Hypoallergenic Enzymatic Hydrolyzate of Cra a 4 with the Potential to Induce Immune Tolerance. Wu, Ming-Xuan Huan, Fei Gao, Shuai Ni, Ling-Na Gu, Yi Han, Tian-Jiao Liu, Meng Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Animals Mice Immune Tolerance Mice, Inbred BALB C Allergens Humans Female CD4-Positive T-Lymphocytes Interleukin-4 Hydrolysis Shellfish Hypersensitivity Sarcoplasmic calcium-binding protein identified in , is a stable allergen; the methods to reduce its allergenicity are still limited. The present study aimed to develop the hypoallergenic enzymatic hydrolyzate of Cra a 4 (ECra a 4) by enzymolysis. After screening of proteases by bioinformatics and optimization of enzymolysis conditions, ECra a 4 was obtained by enzymolysis with Trypsin. Further, ECra a 4 was used to stimulate splenocytes from Cra a 4-sensitized mice, with findings that ECra a 4 could stimulate CD4 T-cell proliferation, inhibit IL-4 secretion, and promote TGF-β secretion. Spectroscopy analysis revealed that enzymolysis destroyed the structure of Cra a 4, which affected its allergenicity. Peptidomic analysis revealed that enzymolysis destroyed the B-cell epitopes of Cra a 4 while retaining its T-cell epitopes. Overall, ECra a 4 with hypoallergenicity and T-cell stimulating ability can be used as an immune tolerance product for further study. |
| format | Artículo científico |
| id | pubmed_39909734 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | Preparation of the Hypoallergenic Enzymatic Hydrolyzate of Cra a 4 with the Potential to Induce Immune Tolerance. Wu, Ming-Xuan Huan, Fei Gao, Shuai Ni, Ling-Na Gu, Yi Han, Tian-Jiao Liu, Meng Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Animals Mice Immune Tolerance Mice, Inbred BALB C Allergens Humans Female CD4-Positive T-Lymphocytes Interleukin-4 Hydrolysis Shellfish Hypersensitivity Preparation of the Hypoallergenic Enzymatic Hydrolyzate of Cra a 4 with the Potential to Induce Immune Tolerance. Wu, Ming-Xuan Huan, Fei Gao, Shuai Ni, Ling-Na Gu, Yi Han, Tian-Jiao Liu, Meng Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Animals Mice Immune Tolerance Mice, Inbred BALB C Allergens Humans Female CD4-Positive T-Lymphocytes Interleukin-4 Hydrolysis Shellfish Hypersensitivity Sarcoplasmic calcium-binding protein identified in , is a stable allergen; the methods to reduce its allergenicity are still limited. The present study aimed to develop the hypoallergenic enzymatic hydrolyzate of Cra a 4 (ECra a 4) by enzymolysis. After screening of proteases by bioinformatics and optimization of enzymolysis conditions, ECra a 4 was obtained by enzymolysis with Trypsin. Further, ECra a 4 was used to stimulate splenocytes from Cra a 4-sensitized mice, with findings that ECra a 4 could stimulate CD4 T-cell proliferation, inhibit IL-4 secretion, and promote TGF-β secretion. Spectroscopy analysis revealed that enzymolysis destroyed the structure of Cra a 4, which affected its allergenicity. Peptidomic analysis revealed that enzymolysis destroyed the B-cell epitopes of Cra a 4 while retaining its T-cell epitopes. Overall, ECra a 4 with hypoallergenicity and T-cell stimulating ability can be used as an immune tolerance product for further study. |
| title | Preparation of the Hypoallergenic Enzymatic Hydrolyzate of Cra a 4 with the Potential to Induce Immune Tolerance. |
| topic | Animals Mice Immune Tolerance Mice, Inbred BALB C Allergens Humans Female CD4-Positive T-Lymphocytes Interleukin-4 Hydrolysis Shellfish Hypersensitivity |
| url | https://pubmed.ncbi.nlm.nih.gov/39909734/ |