Enzymatic degraded polysaccharides from Enteromorpha prolifera inhibit the growth of THP-1 cells and induce apoptosis via the mitochondrial pathway.
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| Format: | Artículo científico |
| Language: | en |
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International journal of biological macromolecules
2025
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| _version_ | 1868266247427194880 |
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| author | Jing, Dannan Su, Yating Xu, Yan He, Zhixiao Hu, Jing Chen, Xinyi Zhou, Zhengrong Zhong, Mingqi Hu, Zhong Li, Jin Mao, Aihua |
| author_facet | Jing, Dannan Su, Yating Xu, Yan He, Zhixiao Hu, Jing Chen, Xinyi Zhou, Zhengrong Zhong, Mingqi Hu, Zhong Li, Jin Mao, Aihua Jing, Dannan Su, Yating Xu, Yan He, Zhixiao Hu, Jing Chen, Xinyi Zhou, Zhengrong Zhong, Mingqi Hu, Zhong Li, Jin Mao, Aihua |
| collection | PubMed - marine biology |
| contents | Enzymatic degraded polysaccharides from Enteromorpha prolifera inhibit the growth of THP-1 cells and induce apoptosis via the mitochondrial pathway. Jing, Dannan Su, Yating Xu, Yan He, Zhixiao Hu, Jing Chen, Xinyi Zhou, Zhengrong Zhong, Mingqi Hu, Zhong Li, Jin Mao, Aihua Humans Apoptosis Mitochondria Polysaccharides THP-1 Cells Cell Proliferation Reactive Oxygen Species Ulva Membrane Potential, Mitochondrial Edible Seaweeds Polysaccharides from Enteromorpha prolifera (EP) is a kind of sulfated polysaccharides with complex structure. Several reports have showed that EP play roles in antioxidant and anti-inflammatory, while, whose function in anti-leukemia is still poorly understood. In this work, EPH were the hydrolysis products of EP by ulvan lyase EPL15085, which displayed lower molecular weight and enhanced sulfate groups than EP. Importantly, EPH exhibited effective inhibitory effect on the human leukemia monocyte cell line, THP-1 cells, however, the EP treated group showed no significant change. Results of JC-1 staining and ROS assay showed mitochondrial dysfunction after EPH treatment, and the increased rate of Bax/Bcl ultimately activated Caspase family proteins to induce apoptosis, indicating the mitochondrial pathway was involved. This study demonstrates the enzymatic products of EP showed enhanced effect on anti-leukemia, which would provide insights for approaches to uncover novel physiological functions of marine polysaccharides and potential therapy for acute myeloid leukemia patients. |
| format | Artículo científico |
| id | pubmed_39914524 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Enzymatic degraded polysaccharides from Enteromorpha prolifera inhibit the growth of THP-1 cells and induce apoptosis via the mitochondrial pathway. Jing, Dannan Su, Yating Xu, Yan He, Zhixiao Hu, Jing Chen, Xinyi Zhou, Zhengrong Zhong, Mingqi Hu, Zhong Li, Jin Mao, Aihua Humans Apoptosis Mitochondria Polysaccharides THP-1 Cells Cell Proliferation Reactive Oxygen Species Ulva Membrane Potential, Mitochondrial Edible Seaweeds Enzymatic degraded polysaccharides from Enteromorpha prolifera inhibit the growth of THP-1 cells and induce apoptosis via the mitochondrial pathway. Jing, Dannan Su, Yating Xu, Yan He, Zhixiao Hu, Jing Chen, Xinyi Zhou, Zhengrong Zhong, Mingqi Hu, Zhong Li, Jin Mao, Aihua Humans Apoptosis Mitochondria Polysaccharides THP-1 Cells Cell Proliferation Reactive Oxygen Species Ulva Membrane Potential, Mitochondrial Edible Seaweeds Polysaccharides from Enteromorpha prolifera (EP) is a kind of sulfated polysaccharides with complex structure. Several reports have showed that EP play roles in antioxidant and anti-inflammatory, while, whose function in anti-leukemia is still poorly understood. In this work, EPH were the hydrolysis products of EP by ulvan lyase EPL15085, which displayed lower molecular weight and enhanced sulfate groups than EP. Importantly, EPH exhibited effective inhibitory effect on the human leukemia monocyte cell line, THP-1 cells, however, the EP treated group showed no significant change. Results of JC-1 staining and ROS assay showed mitochondrial dysfunction after EPH treatment, and the increased rate of Bax/Bcl ultimately activated Caspase family proteins to induce apoptosis, indicating the mitochondrial pathway was involved. This study demonstrates the enzymatic products of EP showed enhanced effect on anti-leukemia, which would provide insights for approaches to uncover novel physiological functions of marine polysaccharides and potential therapy for acute myeloid leukemia patients. |
| title | Enzymatic degraded polysaccharides from Enteromorpha prolifera inhibit the growth of THP-1 cells and induce apoptosis via the mitochondrial pathway. |
| topic | Humans Apoptosis Mitochondria Polysaccharides THP-1 Cells Cell Proliferation Reactive Oxygen Species Ulva Membrane Potential, Mitochondrial Edible Seaweeds |
| url | https://pubmed.ncbi.nlm.nih.gov/39914524/ |