CD3γ/δ T cells and MCSFR macrophages are activated to produce IL-26 after bacterial infection in grass carp.
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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Fish & shellfish immunology
2025
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| _version_ | 1868266244705091585 |
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| author | Xie, Teng Yang, Yibin Chen, Kangyong Huang, Wenji Zhao, Weihua Yan, Hui Qiu, Junqiang Feng, Hao Wang, Junya Zou, Jun |
| author_facet | Xie, Teng Yang, Yibin Chen, Kangyong Huang, Wenji Zhao, Weihua Yan, Hui Qiu, Junqiang Feng, Hao Wang, Junya Zou, Jun Xie, Teng Yang, Yibin Chen, Kangyong Huang, Wenji Zhao, Weihua Yan, Hui Qiu, Junqiang Feng, Hao Wang, Junya Zou, Jun |
| collection | PubMed - marine biology |
| contents | CD3γ/δ T cells and MCSFR macrophages are activated to produce IL-26 after bacterial infection in grass carp. Xie, Teng Yang, Yibin Chen, Kangyong Huang, Wenji Zhao, Weihua Yan, Hui Qiu, Junqiang Feng, Hao Wang, Junya Zou, Jun Animals Carps Aeromonas hydrophila Fish Diseases Gram-Negative Bacterial Infections Fish Proteins Macrophages Interleukins T-Lymphocytes Immunity, Innate HEK293 Cells Humans Interleukin-26 (IL-26) belongs to the IL-10 cytokine family and exerts diverse biological functions in regulating immune responses in vertebrates. Although IL-26 has been extensively studied in mammals, the functions of IL-26 remain largely unexplored in lower vertebrates. In this study, we determined the tissue and cell sources of IL-26 using a monoclonal antibody (mAb) generated against grass carp (Ctenopharyngodon idella, Ci) IL-26, and investigated the responses of IL-26 producing cells to bacterial infection. We showed that the CiIL-26 mAb specifically recognized the recombinant CiIL-26 proteins expressed in the Escherichia coli and HEK293 cells. Flow cytometry analysis revealed that the CiIL-26 mAb could detect the intracellular CiIL-26 expressed in the HEK293 cells and CIK cells stimulated with inactivated Aeromonas hydrophila (A. hydrophila). Using confocal microscopy, we analyzed IL-26 cells in various tissues of grass carp following infection with A. hydrophila. It was shown that the IL-26 cells were significantly increased in the gills, head kidney, posterior intestine and spleen. Remarkably, for the first time, we observed that most IL-26 cells were CD3γ/δ T cells and MCSFR monocytes/macrophages, which could be induced by A. hydrophila. Our findings highlight the essential roles of CD3γ/δIL-26 T cells and MCSFR/IL-26 macrophages in the immune defense against bacterial infections in fish. |
| format | Artículo científico |
| id | pubmed_39938622 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | CD3γ/δ T cells and MCSFR macrophages are activated to produce IL-26 after bacterial infection in grass carp. Xie, Teng Yang, Yibin Chen, Kangyong Huang, Wenji Zhao, Weihua Yan, Hui Qiu, Junqiang Feng, Hao Wang, Junya Zou, Jun Animals Carps Aeromonas hydrophila Fish Diseases Gram-Negative Bacterial Infections Fish Proteins Macrophages Interleukins T-Lymphocytes Immunity, Innate HEK293 Cells Humans CD3γ/δ T cells and MCSFR macrophages are activated to produce IL-26 after bacterial infection in grass carp. Xie, Teng Yang, Yibin Chen, Kangyong Huang, Wenji Zhao, Weihua Yan, Hui Qiu, Junqiang Feng, Hao Wang, Junya Zou, Jun Animals Carps Aeromonas hydrophila Fish Diseases Gram-Negative Bacterial Infections Fish Proteins Macrophages Interleukins T-Lymphocytes Immunity, Innate HEK293 Cells Humans Interleukin-26 (IL-26) belongs to the IL-10 cytokine family and exerts diverse biological functions in regulating immune responses in vertebrates. Although IL-26 has been extensively studied in mammals, the functions of IL-26 remain largely unexplored in lower vertebrates. In this study, we determined the tissue and cell sources of IL-26 using a monoclonal antibody (mAb) generated against grass carp (Ctenopharyngodon idella, Ci) IL-26, and investigated the responses of IL-26 producing cells to bacterial infection. We showed that the CiIL-26 mAb specifically recognized the recombinant CiIL-26 proteins expressed in the Escherichia coli and HEK293 cells. Flow cytometry analysis revealed that the CiIL-26 mAb could detect the intracellular CiIL-26 expressed in the HEK293 cells and CIK cells stimulated with inactivated Aeromonas hydrophila (A. hydrophila). Using confocal microscopy, we analyzed IL-26 cells in various tissues of grass carp following infection with A. hydrophila. It was shown that the IL-26 cells were significantly increased in the gills, head kidney, posterior intestine and spleen. Remarkably, for the first time, we observed that most IL-26 cells were CD3γ/δ T cells and MCSFR monocytes/macrophages, which could be induced by A. hydrophila. Our findings highlight the essential roles of CD3γ/δIL-26 T cells and MCSFR/IL-26 macrophages in the immune defense against bacterial infections in fish. |
| title | CD3γ/δ T cells and MCSFR macrophages are activated to produce IL-26 after bacterial infection in grass carp. |
| topic | Animals Carps Aeromonas hydrophila Fish Diseases Gram-Negative Bacterial Infections Fish Proteins Macrophages Interleukins T-Lymphocytes Immunity, Innate HEK293 Cells Humans |
| url | https://pubmed.ncbi.nlm.nih.gov/39938622/ |