Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Metabolic engineering
2025
|
| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/39947347/ |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1868266242358378497 |
|---|---|
| author | Qiu, Zetian Han, Yumei Li, Jia Ren, Yi Liu, Xue Li, Shengying Zhao, Guang-Rong Du, Lei |
| author_facet | Qiu, Zetian Han, Yumei Li, Jia Ren, Yi Liu, Xue Li, Shengying Zhao, Guang-Rong Du, Lei Qiu, Zetian Han, Yumei Li, Jia Ren, Yi Liu, Xue Li, Shengying Zhao, Guang-Rong Du, Lei |
| collection | PubMed - marine biology |
| contents | Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides. Qiu, Zetian Han, Yumei Li, Jia Ren, Yi Liu, Xue Li, Shengying Zhao, Guang-Rong Du, Lei Escherichia coli Flavonoids Metabolic Engineering Glycosides Heterologous biosynthesis of natural products with long biosynthetic pathways in microorganisms often suffers from diverse problems, such as enzyme promiscuity and metabolic burden. Flavonoids and their glycosides are important phytochemicals in the diet of human beings, with various health benefits and biological activities. Despite previous efforts and achievements, efficient microbial production of plant-derived flavonoid compounds with long pathways remains challenging. Herein, we applied metabolic division engineering of Escherichia coli consortia to overcome these limitations. By establishing new biosynthetic pathways, rationally adjusting metabolic node intermediates, and engineering different auxotrophic and orthogonal carbon sources for hosts, we established stable two- and three-bacteria co-culture systems to efficiently de novo produce 12 flavonoids (61.15-325.31 mg/L) and 36 corresponding flavonoid glycosides (1.31-191.79 mg/L). Furthermore, the co-culture system was rapidly extended in a plug-and-play manner to produce isoflavonoids, dihydrochalcones, and their glycosides. This study successfully alleviates metabolic burden and overcomes enzyme promiscuity, and provides significant insights that could guide the biosynthesis of other complex secondary metabolites. |
| format | Artículo científico |
| id | pubmed_39947347 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Metabolic engineering |
| record_format | pubmed |
| spellingShingle | Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides. Qiu, Zetian Han, Yumei Li, Jia Ren, Yi Liu, Xue Li, Shengying Zhao, Guang-Rong Du, Lei Escherichia coli Flavonoids Metabolic Engineering Glycosides Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides. Qiu, Zetian Han, Yumei Li, Jia Ren, Yi Liu, Xue Li, Shengying Zhao, Guang-Rong Du, Lei Escherichia coli Flavonoids Metabolic Engineering Glycosides Heterologous biosynthesis of natural products with long biosynthetic pathways in microorganisms often suffers from diverse problems, such as enzyme promiscuity and metabolic burden. Flavonoids and their glycosides are important phytochemicals in the diet of human beings, with various health benefits and biological activities. Despite previous efforts and achievements, efficient microbial production of plant-derived flavonoid compounds with long pathways remains challenging. Herein, we applied metabolic division engineering of Escherichia coli consortia to overcome these limitations. By establishing new biosynthetic pathways, rationally adjusting metabolic node intermediates, and engineering different auxotrophic and orthogonal carbon sources for hosts, we established stable two- and three-bacteria co-culture systems to efficiently de novo produce 12 flavonoids (61.15-325.31 mg/L) and 36 corresponding flavonoid glycosides (1.31-191.79 mg/L). Furthermore, the co-culture system was rapidly extended in a plug-and-play manner to produce isoflavonoids, dihydrochalcones, and their glycosides. This study successfully alleviates metabolic burden and overcomes enzyme promiscuity, and provides significant insights that could guide the biosynthesis of other complex secondary metabolites. |
| title | Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides. |
| topic | Escherichia coli Flavonoids Metabolic Engineering Glycosides |
| url | https://pubmed.ncbi.nlm.nih.gov/39947347/ |