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| Main Authors: | , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Communications chemistry
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39953270/ |
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| _version_ | 1868266242349989889 |
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| author | Osborn Popp, Thomas M Karthikeyan, Mithun Herman, Elias M Dufur, Andrew C Vetriani, Costantino Nieuwkoop, Andrew J |
| author_facet | Osborn Popp, Thomas M Karthikeyan, Mithun Herman, Elias M Dufur, Andrew C Vetriani, Costantino Nieuwkoop, Andrew J Osborn Popp, Thomas M Karthikeyan, Mithun Herman, Elias M Dufur, Andrew C Vetriani, Costantino Nieuwkoop, Andrew J |
| collection | PubMed - marine biology |
| contents | Measurement of phospholipid lateral diffusion at high pressure by in situ magic-angle spinning NMR spectroscopy. Osborn Popp, Thomas M Karthikeyan, Mithun Herman, Elias M Dufur, Andrew C Vetriani, Costantino Nieuwkoop, Andrew J The development of experimental methodologies that enable investigations of biochemistry at high pressure promises to yield significant advances in our understanding of life on Earth and its origins. Here, we introduce a method for studying lipid membranes at thermodynamic conditions relevant for life at deep sea hydrothermal vents. Using in situ high pressure magic-angle spinning solid state nuclear magnetic resonance spectroscopy (NMR), we measure changes in the fluidity of model microbial membranes at pressures up to 28 MPa. We find that the fluid-phase lateral diffusion of phospholipids at high pressure is significantly affected by the stoichiometric ratio of lipids in the membrane. Our results were facilitated by an accessible pressurization strategy that we have developed to enable routine preparation of solid state NMR rotors to pressures of 30 MPa or greater. |
| format | Artículo científico |
| id | pubmed_39953270 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Communications chemistry |
| record_format | pubmed |
| spellingShingle | Measurement of phospholipid lateral diffusion at high pressure by in situ magic-angle spinning NMR spectroscopy. Osborn Popp, Thomas M Karthikeyan, Mithun Herman, Elias M Dufur, Andrew C Vetriani, Costantino Nieuwkoop, Andrew J Measurement of phospholipid lateral diffusion at high pressure by in situ magic-angle spinning NMR spectroscopy. Osborn Popp, Thomas M Karthikeyan, Mithun Herman, Elias M Dufur, Andrew C Vetriani, Costantino Nieuwkoop, Andrew J The development of experimental methodologies that enable investigations of biochemistry at high pressure promises to yield significant advances in our understanding of life on Earth and its origins. Here, we introduce a method for studying lipid membranes at thermodynamic conditions relevant for life at deep sea hydrothermal vents. Using in situ high pressure magic-angle spinning solid state nuclear magnetic resonance spectroscopy (NMR), we measure changes in the fluidity of model microbial membranes at pressures up to 28 MPa. We find that the fluid-phase lateral diffusion of phospholipids at high pressure is significantly affected by the stoichiometric ratio of lipids in the membrane. Our results were facilitated by an accessible pressurization strategy that we have developed to enable routine preparation of solid state NMR rotors to pressures of 30 MPa or greater. |
| title | Measurement of phospholipid lateral diffusion at high pressure by in situ magic-angle spinning NMR spectroscopy. |
| url | https://pubmed.ncbi.nlm.nih.gov/39953270/ |