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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Frontiers in immunology
2025
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/39963139/ |
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| author | Stryiński, Robert Fiedorowicz, Ewa Mateos, Jesús Andronowska, Aneta Łopieńska-Biernat, Elżbieta Carrera, Mónica |
| author_facet | Stryiński, Robert Fiedorowicz, Ewa Mateos, Jesús Andronowska, Aneta Łopieńska-Biernat, Elżbieta Carrera, Mónica Stryiński, Robert Fiedorowicz, Ewa Mateos, Jesús Andronowska, Aneta Łopieńska-Biernat, Elżbieta Carrera, Mónica |
| collection | PubMed - marine biology |
| contents | Exploring the exoproteome of the parasitic nematode (s. s.) and its impact on the human host - an cross-talk proteomic approach. Stryiński, Robert Fiedorowicz, Ewa Mateos, Jesús Andronowska, Aneta Łopieńska-Biernat, Elżbieta Carrera, Mónica Anisakis Humans Animals Proteomics Proteome Caco-2 Cells Host-Parasite Interactions Extracellular Vesicles Helminth Proteins Larva Anisakiasis Coculture Techniques Anisakis simplex sensu stricto (s. s.) is one of the most widespread parasitic nematodes of marine organisms, with humans as accidental hosts. While many studies have explored nematode biology and host interactions, the role of extracellular vesicles (EVs) as signaling molecules in parasitic nematodes is less understood. Therefore, the proteins present in the EVs of A. simplex (s. s.) (Anis-EVs) were identified. In addition, a cross-talk proteomic approach was used to identify differentially regulated proteins (DRPs) in the proteome of the human intestinal epithelial cell line (Caco-2) co-cultured with L3 larvae of A. simplex (s. s.) or directly exposed to two concentrations (low or high) of Anis-EVs. In addition, DRPs were identified in the proteome of A. simplex (s. s.) larvae affected by co-culture with Caco-2. To achieve this goal, the shotgun proteomics method based on isobaric mass labeling (via tandem mass tags; TMT) was used with a combination of nano high-performance liquid chromatography (nLC) coupled with an LTQ-Orbitrap Elite mass spectrometer. In addition, ELISA assays were used to demonstrate if Caco-2 respond to A. simplex (s. s.) larvae and Anis-EVs with significant changes in selected cytokines secretion. The results of this study indicate the anti-inflammatory character of Anis-EVs in relation to Caco-2. At the same time, direct treatment with Anis-EVs resulted in more significant changes in the Caco-2 proteome than co-culture with L3 larvae. The results obtained should lead to a better understanding of the molecular mechanisms underlying the development of A. simplex (s. s.) infection in humans and will complement the existing knowledge on the role of EVs in host-parasite communication. |
| format | Artículo científico |
| id | pubmed_39963139 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Frontiers in immunology |
| record_format | pubmed |
| spellingShingle | Exploring the exoproteome of the parasitic nematode (s. s.) and its impact on the human host - an cross-talk proteomic approach. Stryiński, Robert Fiedorowicz, Ewa Mateos, Jesús Andronowska, Aneta Łopieńska-Biernat, Elżbieta Carrera, Mónica Anisakis Humans Animals Proteomics Proteome Caco-2 Cells Host-Parasite Interactions Extracellular Vesicles Helminth Proteins Larva Anisakiasis Coculture Techniques Exploring the exoproteome of the parasitic nematode (s. s.) and its impact on the human host - an cross-talk proteomic approach. Stryiński, Robert Fiedorowicz, Ewa Mateos, Jesús Andronowska, Aneta Łopieńska-Biernat, Elżbieta Carrera, Mónica Anisakis Humans Animals Proteomics Proteome Caco-2 Cells Host-Parasite Interactions Extracellular Vesicles Helminth Proteins Larva Anisakiasis Coculture Techniques Anisakis simplex sensu stricto (s. s.) is one of the most widespread parasitic nematodes of marine organisms, with humans as accidental hosts. While many studies have explored nematode biology and host interactions, the role of extracellular vesicles (EVs) as signaling molecules in parasitic nematodes is less understood. Therefore, the proteins present in the EVs of A. simplex (s. s.) (Anis-EVs) were identified. In addition, a cross-talk proteomic approach was used to identify differentially regulated proteins (DRPs) in the proteome of the human intestinal epithelial cell line (Caco-2) co-cultured with L3 larvae of A. simplex (s. s.) or directly exposed to two concentrations (low or high) of Anis-EVs. In addition, DRPs were identified in the proteome of A. simplex (s. s.) larvae affected by co-culture with Caco-2. To achieve this goal, the shotgun proteomics method based on isobaric mass labeling (via tandem mass tags; TMT) was used with a combination of nano high-performance liquid chromatography (nLC) coupled with an LTQ-Orbitrap Elite mass spectrometer. In addition, ELISA assays were used to demonstrate if Caco-2 respond to A. simplex (s. s.) larvae and Anis-EVs with significant changes in selected cytokines secretion. The results of this study indicate the anti-inflammatory character of Anis-EVs in relation to Caco-2. At the same time, direct treatment with Anis-EVs resulted in more significant changes in the Caco-2 proteome than co-culture with L3 larvae. The results obtained should lead to a better understanding of the molecular mechanisms underlying the development of A. simplex (s. s.) infection in humans and will complement the existing knowledge on the role of EVs in host-parasite communication. |
| title | Exploring the exoproteome of the parasitic nematode (s. s.) and its impact on the human host - an cross-talk proteomic approach. |
| topic | Anisakis Humans Animals Proteomics Proteome Caco-2 Cells Host-Parasite Interactions Extracellular Vesicles Helminth Proteins Larva Anisakiasis Coculture Techniques |
| url | https://pubmed.ncbi.nlm.nih.gov/39963139/ |