Phyloproteomics reveals conserved patterns of axonemal dynein methylation across the motile ciliated eukaryotes.
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Molecular biology of the cell
2025
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| author | Sakato-Antoku, Miho Patel, Nikisha Inaba, Mayu Rao, Qinhui Yang, Jun Patel-King, Ramila S Inaba, Kazuo Balsbaugh, Jeremy L King, Stephen M |
| author_facet | Sakato-Antoku, Miho Patel, Nikisha Inaba, Mayu Rao, Qinhui Yang, Jun Patel-King, Ramila S Inaba, Kazuo Balsbaugh, Jeremy L King, Stephen M Sakato-Antoku, Miho Patel, Nikisha Inaba, Mayu Rao, Qinhui Yang, Jun Patel-King, Ramila S Inaba, Kazuo Balsbaugh, Jeremy L King, Stephen M |
| collection | PubMed - marine biology |
| contents | Phyloproteomics reveals conserved patterns of axonemal dynein methylation across the motile ciliated eukaryotes. Sakato-Antoku, Miho Patel, Nikisha Inaba, Mayu Rao, Qinhui Yang, Jun Patel-King, Ramila S Inaba, Kazuo Balsbaugh, Jeremy L King, Stephen M Animals Methylation Axonemal Dyneins Proteomics Cilia Drosophila melanogaster Axoneme Phylogeny Chlamydomonas Eukaryota Male Axonemal dynein assembly occurs in the cytoplasm and numerous cytosolic factors are specifically required for this process. Recently, one factor (DNAAF3/PF22) was identified as a methyltransferase. Examination of dyneins found they are methylated at substoichiometric levels on multiple sites, including Lys and Arg residues in several of the nucleotide-binding domains and on the microtubule-binding region. Given the highly conserved nature of axonemal dyneins, one key question is whether methylation happens only in dyneins from the chlorophyte algae, or whether these modifications occur more broadly throughout the motile ciliated eukaryotes. Here we take a phyloproteomic approach and examine dynein methylation in a wide range of eukaryotic organisms bearing motile cilia. We find unambiguous evidence for methylation of axonemal dyneins in alveolates, chlorophytes, trypanosomes, and a broad range of metazoans. Intriguingly, we were unable to identify a single instance of methylation on sperm dyneins even though dipterans express a Dnaaf3 orthologue, or in spermatozoids of the fern , which assembles inner arms but lacks both outer arm dyneins and DNAAF3. Thus, methylation of axonemal dyneins has been broadly conserved in most eukaryotic groups and has the potential to variably modify the function of these motors. |
| format | Artículo científico |
| id | pubmed_39969973 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Molecular biology of the cell |
| record_format | pubmed |
| spellingShingle | Phyloproteomics reveals conserved patterns of axonemal dynein methylation across the motile ciliated eukaryotes. Sakato-Antoku, Miho Patel, Nikisha Inaba, Mayu Rao, Qinhui Yang, Jun Patel-King, Ramila S Inaba, Kazuo Balsbaugh, Jeremy L King, Stephen M Animals Methylation Axonemal Dyneins Proteomics Cilia Drosophila melanogaster Axoneme Phylogeny Chlamydomonas Eukaryota Male Phyloproteomics reveals conserved patterns of axonemal dynein methylation across the motile ciliated eukaryotes. Sakato-Antoku, Miho Patel, Nikisha Inaba, Mayu Rao, Qinhui Yang, Jun Patel-King, Ramila S Inaba, Kazuo Balsbaugh, Jeremy L King, Stephen M Animals Methylation Axonemal Dyneins Proteomics Cilia Drosophila melanogaster Axoneme Phylogeny Chlamydomonas Eukaryota Male Axonemal dynein assembly occurs in the cytoplasm and numerous cytosolic factors are specifically required for this process. Recently, one factor (DNAAF3/PF22) was identified as a methyltransferase. Examination of dyneins found they are methylated at substoichiometric levels on multiple sites, including Lys and Arg residues in several of the nucleotide-binding domains and on the microtubule-binding region. Given the highly conserved nature of axonemal dyneins, one key question is whether methylation happens only in dyneins from the chlorophyte algae, or whether these modifications occur more broadly throughout the motile ciliated eukaryotes. Here we take a phyloproteomic approach and examine dynein methylation in a wide range of eukaryotic organisms bearing motile cilia. We find unambiguous evidence for methylation of axonemal dyneins in alveolates, chlorophytes, trypanosomes, and a broad range of metazoans. Intriguingly, we were unable to identify a single instance of methylation on sperm dyneins even though dipterans express a Dnaaf3 orthologue, or in spermatozoids of the fern , which assembles inner arms but lacks both outer arm dyneins and DNAAF3. Thus, methylation of axonemal dyneins has been broadly conserved in most eukaryotic groups and has the potential to variably modify the function of these motors. |
| title | Phyloproteomics reveals conserved patterns of axonemal dynein methylation across the motile ciliated eukaryotes. |
| topic | Animals Methylation Axonemal Dyneins Proteomics Cilia Drosophila melanogaster Axoneme Phylogeny Chlamydomonas Eukaryota Male |
| url | https://pubmed.ncbi.nlm.nih.gov/39969973/ |