Study on the Mechanism by Which Fe Promotes Toluene Degradation by sp. TG-1.
Fuente:
PubMed
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Microorganisms
2025
|
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1868266237335699457 |
|---|---|
| author | Qiao, Yue Ma, Jiajun Huang, Lei Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong |
| author_facet | Qiao, Yue Ma, Jiajun Huang, Lei Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Qiao, Yue Ma, Jiajun Huang, Lei Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong |
| collection | PubMed - marine biology |
| contents | Study on the Mechanism by Which Fe Promotes Toluene Degradation by sp. TG-1. Qiao, Yue Ma, Jiajun Huang, Lei Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Volatile organic compound pollution caused by toluene has become a global issue. In order to solve this problem, biodegradation of toluene has been applied all over the world. This study investigated the effects of Fe on toluene degradation by the sp. TG-1. The results show that 1 mg L Fe increased the degradation rate of 600 mg L toluene from 61.9% to 87.2% at 16 h. The acceleration mechanism of Fe was explicated using transmission electron microscope (TEM) and energy-dispersive X-ray spectroscopy (EDX) analyses, coupled plasma optical emission spectroscopy, an enzyme activity assay, and transcriptome analysis. Four genes were detected to be significantly up-regulated under Fe induction, suggesting that Fe might be implicated in toluene degradation. Meanwhile, Fe was a component of the active center of catechol 1,2-dioxygenase (C12O) and significantly improved the enzyme activity of C12O. The mechanism by which Fe accelerates toluene degradation was proposed based on the transcription levels of degradation genes and the enzyme activity of C12O. This study provided an improved method for enhancing the degradation effect of toluene and furthered our comprehension of the mechanism of toluene degradation. |
| format | Artículo científico |
| id | pubmed_40005833 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Study on the Mechanism by Which Fe Promotes Toluene Degradation by sp. TG-1. Qiao, Yue Ma, Jiajun Huang, Lei Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Study on the Mechanism by Which Fe Promotes Toluene Degradation by sp. TG-1. Qiao, Yue Ma, Jiajun Huang, Lei Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Volatile organic compound pollution caused by toluene has become a global issue. In order to solve this problem, biodegradation of toluene has been applied all over the world. This study investigated the effects of Fe on toluene degradation by the sp. TG-1. The results show that 1 mg L Fe increased the degradation rate of 600 mg L toluene from 61.9% to 87.2% at 16 h. The acceleration mechanism of Fe was explicated using transmission electron microscope (TEM) and energy-dispersive X-ray spectroscopy (EDX) analyses, coupled plasma optical emission spectroscopy, an enzyme activity assay, and transcriptome analysis. Four genes were detected to be significantly up-regulated under Fe induction, suggesting that Fe might be implicated in toluene degradation. Meanwhile, Fe was a component of the active center of catechol 1,2-dioxygenase (C12O) and significantly improved the enzyme activity of C12O. The mechanism by which Fe accelerates toluene degradation was proposed based on the transcription levels of degradation genes and the enzyme activity of C12O. This study provided an improved method for enhancing the degradation effect of toluene and furthered our comprehension of the mechanism of toluene degradation. |
| title | Study on the Mechanism by Which Fe Promotes Toluene Degradation by sp. TG-1. |
| url | https://pubmed.ncbi.nlm.nih.gov/40005833/ |