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| Main Authors: | , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Chemical biology & drug design
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40007225/ |
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| _version_ | 1868266237335699456 |
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| author | Yamaguchi, Masayoshi Yoshiike, Kenji Watanabe, Hideaki Watanabe, Mitsugu |
| author_facet | Yamaguchi, Masayoshi Yoshiike, Kenji Watanabe, Hideaki Watanabe, Mitsugu Yamaguchi, Masayoshi Yoshiike, Kenji Watanabe, Hideaki Watanabe, Mitsugu |
| collection | PubMed - marine biology |
| contents | The Potent Antioxidant 3,5-Dihydroxy-4-Methoxybenzyl Alcohol Reveals Anticancer Activity by Targeting Several Signaling Pathways in Bone Metastatic Human Breast Cancer MDA-MB-231 Cells. Yamaguchi, Masayoshi Yoshiike, Kenji Watanabe, Hideaki Watanabe, Mitsugu Humans Signal Transduction Cell Line, Tumor Female Mice Antineoplastic Agents Breast Neoplasms Animals Bone Neoplasms Antioxidants Cell Proliferation Benzyl Alcohols Cell Movement RAW 264.7 Cells MDA-MB-231 Cells Human breast cancer is the leading cause of cancer-related death in women. Bone metastatic human breast cancer MDA-MB-231 cells are triple negative. The novel marine factor 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), a potent antioxidant, has been shown to prevent oxidative stress by scavenging free radicals in cells. This study investigates the effects of DHMBA on MDA-MB-231 cells in vitro. MDA-MB-231 cells were cultured with DHMBA (0.1-100 μM). DHMBA blocked the growth and stimulated the death of MDA-MB-231 cells, resulting in reduced cell numbers. DHMBA treatment decreased PI3-kinase 100α, Akt, MAPK, phosphor-MAPK, and mTOR and increased p53, p21, and Rb, which are suppressors in cell growth. DHMBA inhibited metastatic activity, including adhesion and migration of MDA-MB-231 cells. Coculture with MDA-MB-231 cells resulted in decreased growth and stimulated death of osteoblastic MC3T3-E1 cells and macrophage RAW264.7 cells, suggesting that cancer cells affect the bone microenvironment. Production of TNF-α, which is the mediator in the bone microenvironment, in MDA-MB-231 cells was inhibited by DHMBA treatment. Crosstalk between cancer cells and cells in the bone microenvironment was blocked by culture with DHMBA. DHMBA may inhibit the activity of triple-negative human breast cancer cells, providing a useful tool for the treatment of breast cancer. |
| format | Artículo científico |
| id | pubmed_40007225 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Chemical biology & drug design |
| record_format | pubmed |
| spellingShingle | The Potent Antioxidant 3,5-Dihydroxy-4-Methoxybenzyl Alcohol Reveals Anticancer Activity by Targeting Several Signaling Pathways in Bone Metastatic Human Breast Cancer MDA-MB-231 Cells. Yamaguchi, Masayoshi Yoshiike, Kenji Watanabe, Hideaki Watanabe, Mitsugu Humans Signal Transduction Cell Line, Tumor Female Mice Antineoplastic Agents Breast Neoplasms Animals Bone Neoplasms Antioxidants Cell Proliferation Benzyl Alcohols Cell Movement RAW 264.7 Cells MDA-MB-231 Cells The Potent Antioxidant 3,5-Dihydroxy-4-Methoxybenzyl Alcohol Reveals Anticancer Activity by Targeting Several Signaling Pathways in Bone Metastatic Human Breast Cancer MDA-MB-231 Cells. Yamaguchi, Masayoshi Yoshiike, Kenji Watanabe, Hideaki Watanabe, Mitsugu Humans Signal Transduction Cell Line, Tumor Female Mice Antineoplastic Agents Breast Neoplasms Animals Bone Neoplasms Antioxidants Cell Proliferation Benzyl Alcohols Cell Movement RAW 264.7 Cells MDA-MB-231 Cells Human breast cancer is the leading cause of cancer-related death in women. Bone metastatic human breast cancer MDA-MB-231 cells are triple negative. The novel marine factor 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), a potent antioxidant, has been shown to prevent oxidative stress by scavenging free radicals in cells. This study investigates the effects of DHMBA on MDA-MB-231 cells in vitro. MDA-MB-231 cells were cultured with DHMBA (0.1-100 μM). DHMBA blocked the growth and stimulated the death of MDA-MB-231 cells, resulting in reduced cell numbers. DHMBA treatment decreased PI3-kinase 100α, Akt, MAPK, phosphor-MAPK, and mTOR and increased p53, p21, and Rb, which are suppressors in cell growth. DHMBA inhibited metastatic activity, including adhesion and migration of MDA-MB-231 cells. Coculture with MDA-MB-231 cells resulted in decreased growth and stimulated death of osteoblastic MC3T3-E1 cells and macrophage RAW264.7 cells, suggesting that cancer cells affect the bone microenvironment. Production of TNF-α, which is the mediator in the bone microenvironment, in MDA-MB-231 cells was inhibited by DHMBA treatment. Crosstalk between cancer cells and cells in the bone microenvironment was blocked by culture with DHMBA. DHMBA may inhibit the activity of triple-negative human breast cancer cells, providing a useful tool for the treatment of breast cancer. |
| title | The Potent Antioxidant 3,5-Dihydroxy-4-Methoxybenzyl Alcohol Reveals Anticancer Activity by Targeting Several Signaling Pathways in Bone Metastatic Human Breast Cancer MDA-MB-231 Cells. |
| topic | Humans Signal Transduction Cell Line, Tumor Female Mice Antineoplastic Agents Breast Neoplasms Animals Bone Neoplasms Antioxidants Cell Proliferation Benzyl Alcohols Cell Movement RAW 264.7 Cells MDA-MB-231 Cells |
| url | https://pubmed.ncbi.nlm.nih.gov/40007225/ |