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Main Authors:	Boukhchtaber, Dina Castillo, von Meijenfeldt, F A Bastiaan, Sahonero Canavesi, Diana X, Dorhout, Denise, Bale, Nicole J, Hopmans, Ellen C, Villanueva, Laura
Format:	Artículo científico
Language:	en
Published:	Environmental microbiology 2025
Subjects:	Archaea Bacteria Membrane Lipids Seawater Black Sea Metagenome
Online Access:	https://pubmed.ncbi.nlm.nih.gov/40016913/
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author	Boukhchtaber, Dina Castillo von Meijenfeldt, F A Bastiaan Sahonero Canavesi, Diana X Dorhout, Denise Bale, Nicole J Hopmans, Ellen C Villanueva, Laura
author_facet	Boukhchtaber, Dina Castillo von Meijenfeldt, F A Bastiaan Sahonero Canavesi, Diana X Dorhout, Denise Bale, Nicole J Hopmans, Ellen C Villanueva, Laura Boukhchtaber, Dina Castillo von Meijenfeldt, F A Bastiaan Sahonero Canavesi, Diana X Dorhout, Denise Bale, Nicole J Hopmans, Ellen C Villanueva, Laura
collection	PubMed - marine biology
contents	Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System. Boukhchtaber, Dina Castillo von Meijenfeldt, F A Bastiaan Sahonero Canavesi, Diana X Dorhout, Denise Bale, Nicole J Hopmans, Ellen C Villanueva, Laura Archaea Bacteria Membrane Lipids Seawater Black Sea Metagenome Bacterial membrane lipids are typically characterised by fatty acid bilayers linked through ester bonds, whereas those of Archaea are characterised by ether-linked isoprenoids forming bilayers or monolayers of membrane-spanning lipids known as isoprenoidal glycerol dialkyl glycerol tetraethers (isoGDGTs). However, this understanding has been reconsidered with the identification of branched GDGTs (brGDGTs), which are membrane-spanning ether-bound branched alkyl fatty acids of bacterial origin, though their producers are often unidentified. The limited availability of microbial cultures constrains the understanding of the biological sources of these membrane lipids, thus limiting their use as biomarkers. To address this issue, we identified membrane lipids in the Black Sea using high-resolution accurate mass/mass spectrometry and inferred their potential producers by targeting lipid biosynthetic pathways encoded on the metagenome, in metagenome-assembled genomes and unbinned scaffolds. We also identified brGDGTs and highly branched GDGTs in the suboxic and euxinic waters, potentially attributed to Planctomycetota, Cloacimonadota, Desulfobacterota, Chloroflexota, Actinobacteria and Myxococcota-based on their lipid biosynthetic genomic potential. These findings introduce new possibilities for using specific brGDGTs as biomarkers of anoxic conditions in marine environments and highlight the role of these membrane lipids in microbial adaptation.
format	Artículo científico
id	pubmed_40016913
institution	PubMed
language	en
publishDate	2025
publisher	Environmental microbiology
record_format	pubmed
spellingShingle	Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System. Boukhchtaber, Dina Castillo von Meijenfeldt, F A Bastiaan Sahonero Canavesi, Diana X Dorhout, Denise Bale, Nicole J Hopmans, Ellen C Villanueva, Laura Archaea Bacteria Membrane Lipids Seawater Black Sea Metagenome Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System. Boukhchtaber, Dina Castillo von Meijenfeldt, F A Bastiaan Sahonero Canavesi, Diana X Dorhout, Denise Bale, Nicole J Hopmans, Ellen C Villanueva, Laura Archaea Bacteria Membrane Lipids Seawater Black Sea Metagenome Bacterial membrane lipids are typically characterised by fatty acid bilayers linked through ester bonds, whereas those of Archaea are characterised by ether-linked isoprenoids forming bilayers or monolayers of membrane-spanning lipids known as isoprenoidal glycerol dialkyl glycerol tetraethers (isoGDGTs). However, this understanding has been reconsidered with the identification of branched GDGTs (brGDGTs), which are membrane-spanning ether-bound branched alkyl fatty acids of bacterial origin, though their producers are often unidentified. The limited availability of microbial cultures constrains the understanding of the biological sources of these membrane lipids, thus limiting their use as biomarkers. To address this issue, we identified membrane lipids in the Black Sea using high-resolution accurate mass/mass spectrometry and inferred their potential producers by targeting lipid biosynthetic pathways encoded on the metagenome, in metagenome-assembled genomes and unbinned scaffolds. We also identified brGDGTs and highly branched GDGTs in the suboxic and euxinic waters, potentially attributed to Planctomycetota, Cloacimonadota, Desulfobacterota, Chloroflexota, Actinobacteria and Myxococcota-based on their lipid biosynthetic genomic potential. These findings introduce new possibilities for using specific brGDGTs as biomarkers of anoxic conditions in marine environments and highlight the role of these membrane lipids in microbial adaptation.
title	Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System.
topic	Archaea Bacteria Membrane Lipids Seawater Black Sea Metagenome
url	https://pubmed.ncbi.nlm.nih.gov/40016913/

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