Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy.
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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
2025
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| author | Barroso-Solares, Suset Lopez-Moya, Federico Fraile, Teresa Prieto, Ángel Carmelo Lopez-Llorca, Luis Pinto, Javier |
| author_facet | Barroso-Solares, Suset Lopez-Moya, Federico Fraile, Teresa Prieto, Ángel Carmelo Lopez-Llorca, Luis Pinto, Javier Barroso-Solares, Suset Lopez-Moya, Federico Fraile, Teresa Prieto, Ángel Carmelo Lopez-Llorca, Luis Pinto, Javier |
| collection | PubMed - marine biology |
| contents | Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy. Barroso-Solares, Suset Lopez-Moya, Federico Fraile, Teresa Prieto, Ángel Carmelo Lopez-Llorca, Luis Pinto, Javier Chitin Spectrum Analysis, Raman Cell Wall Chitosan Hypocreales Ascomycota Investigation of cell wall composition is necessary to understand the interactions between fungi and the environment as it is the external layer exposed to stimuli and detected by other organisms. Pochonia chlamydosporia and Akanthomyces lecanii, two fungal species living in the soil and infecting nematodes and insects, exhibit endophytic interactions with various plant species. Determination of cell wall composition is essential to understand the mechanisms underlying these interactions. Therefore, in this study, for the first time, we assessed the relative amounts of chitin and chitosan in the cell walls of P. chlamydosporia (PC123) and A. lecanii (69NZ, 85SCT, 126KNY, and 447SAF) via Raman spectroscopy. The isolate with the highest chitosan percentage was 69NZ, followed by 85SCT, PC123, 447SAF, and 126KNY. Moreover, combination with conventional approaches for chitin and chitosan quantification yielded quantitative results for all cell wall components. Overall, these results highlight the mechanisms by which fungi exhibit chitosan resistance and avoid detection by the host plant during root colonization. |
| format | Artículo científico |
| id | pubmed_40024085 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy |
| record_format | pubmed |
| spellingShingle | Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy. Barroso-Solares, Suset Lopez-Moya, Federico Fraile, Teresa Prieto, Ángel Carmelo Lopez-Llorca, Luis Pinto, Javier Chitin Spectrum Analysis, Raman Cell Wall Chitosan Hypocreales Ascomycota Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy. Barroso-Solares, Suset Lopez-Moya, Federico Fraile, Teresa Prieto, Ángel Carmelo Lopez-Llorca, Luis Pinto, Javier Chitin Spectrum Analysis, Raman Cell Wall Chitosan Hypocreales Ascomycota Investigation of cell wall composition is necessary to understand the interactions between fungi and the environment as it is the external layer exposed to stimuli and detected by other organisms. Pochonia chlamydosporia and Akanthomyces lecanii, two fungal species living in the soil and infecting nematodes and insects, exhibit endophytic interactions with various plant species. Determination of cell wall composition is essential to understand the mechanisms underlying these interactions. Therefore, in this study, for the first time, we assessed the relative amounts of chitin and chitosan in the cell walls of P. chlamydosporia (PC123) and A. lecanii (69NZ, 85SCT, 126KNY, and 447SAF) via Raman spectroscopy. The isolate with the highest chitosan percentage was 69NZ, followed by 85SCT, PC123, 447SAF, and 126KNY. Moreover, combination with conventional approaches for chitin and chitosan quantification yielded quantitative results for all cell wall components. Overall, these results highlight the mechanisms by which fungi exhibit chitosan resistance and avoid detection by the host plant during root colonization. |
| title | Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy. |
| topic | Chitin Spectrum Analysis, Raman Cell Wall Chitosan Hypocreales Ascomycota |
| url | https://pubmed.ncbi.nlm.nih.gov/40024085/ |