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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Bioscience, biotechnology, and biochemistry
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40037896/ |
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| _version_ | 1868266234576896001 |
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| author | Chen, Nannan Kishida, Kouhei Stari, Leonardo Moriuchi, Ryota Ohtsubo, Yoshiyuki Damborsky, Jiri Nagata, Yuji |
| author_facet | Chen, Nannan Kishida, Kouhei Stari, Leonardo Moriuchi, Ryota Ohtsubo, Yoshiyuki Damborsky, Jiri Nagata, Yuji Chen, Nannan Kishida, Kouhei Stari, Leonardo Moriuchi, Ryota Ohtsubo, Yoshiyuki Damborsky, Jiri Nagata, Yuji |
| collection | PubMed - marine biology |
| contents | Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization. Chen, Nannan Kishida, Kouhei Stari, Leonardo Moriuchi, Ryota Ohtsubo, Yoshiyuki Damborsky, Jiri Nagata, Yuji Hexachlorocyclohexane Hydrolases Sphingomonadaceae Phylogeny Bacterial Proteins Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of γ-hexachlorocyclohexane (γ-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward γ-HCH utilization, the linB gene in γ-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from γ-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with γ-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the γ-HCH utilization. |
| format | Artículo científico |
| id | pubmed_40037896 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Bioscience, biotechnology, and biochemistry |
| record_format | pubmed |
| spellingShingle | Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization. Chen, Nannan Kishida, Kouhei Stari, Leonardo Moriuchi, Ryota Ohtsubo, Yoshiyuki Damborsky, Jiri Nagata, Yuji Hexachlorocyclohexane Hydrolases Sphingomonadaceae Phylogeny Bacterial Proteins Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization. Chen, Nannan Kishida, Kouhei Stari, Leonardo Moriuchi, Ryota Ohtsubo, Yoshiyuki Damborsky, Jiri Nagata, Yuji Hexachlorocyclohexane Hydrolases Sphingomonadaceae Phylogeny Bacterial Proteins Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of γ-hexachlorocyclohexane (γ-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward γ-HCH utilization, the linB gene in γ-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from γ-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with γ-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the γ-HCH utilization. |
| title | Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization. |
| topic | Hexachlorocyclohexane Hydrolases Sphingomonadaceae Phylogeny Bacterial Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/40037896/ |