Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens.
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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of basic microbiology
2025
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| _version_ | 1868266234560118784 |
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| author | Zhou, Tianyu Cao, Yuan Zhang, Zhicheng Shi, Jing Ge, Huiming An, Faliang Ye, Jiang Wang, Ruida Wu, Haizhen |
| author_facet | Zhou, Tianyu Cao, Yuan Zhang, Zhicheng Shi, Jing Ge, Huiming An, Faliang Ye, Jiang Wang, Ruida Wu, Haizhen Zhou, Tianyu Cao, Yuan Zhang, Zhicheng Shi, Jing Ge, Huiming An, Faliang Ye, Jiang Wang, Ruida Wu, Haizhen |
| collection | PubMed - marine biology |
| contents | Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens. Zhou, Tianyu Cao, Yuan Zhang, Zhicheng Shi, Jing Ge, Huiming An, Faliang Ye, Jiang Wang, Ruida Wu, Haizhen Fermentation Actinobacteria Culture Media Bioreactors Antineoplastic Agents Tryptophan Shikimic Acid Aquatic Organisms Gene Expression Profiling Biosynthetic Pathways The antitumor drug candidate, loonamycin, was isolated from Nocardiopsis flavescens NA01583 and exerts its antitumor activity by inhibiting topoisomerase I activity. However, the current production levels of loonamycin are insufficient for further research. To address this limitation, a liquid fermentation method was developed, optimizing both fermentation conditions and medium components. This optimization increased loonamycin production from 1 mg/mL on agar plate to 602.32 mg/mL in shake flask and ultimately 532.51 mg/mL in a 5-L bioreactor. Exogenous addition experiments revealed that sea salt and metal ions significantly inhibit loonamycin synthesis. Multi-temporal transcriptomic analyses indicated that the reduced expression levels of genes involved in the shikimate pathway and L-tryptophan biosynthesis pathway were the limiting factors for increased loonamycin yield. Overall, an effective fermentation method for rare marine actinomycetes was established, significantly enhancing loonamycin production and providing a foundation for the development of this novel antitumor prodrug. |
| format | Artículo científico |
| id | pubmed_40047177 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of basic microbiology |
| record_format | pubmed |
| spellingShingle | Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens. Zhou, Tianyu Cao, Yuan Zhang, Zhicheng Shi, Jing Ge, Huiming An, Faliang Ye, Jiang Wang, Ruida Wu, Haizhen Fermentation Actinobacteria Culture Media Bioreactors Antineoplastic Agents Tryptophan Shikimic Acid Aquatic Organisms Gene Expression Profiling Biosynthetic Pathways Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens. Zhou, Tianyu Cao, Yuan Zhang, Zhicheng Shi, Jing Ge, Huiming An, Faliang Ye, Jiang Wang, Ruida Wu, Haizhen Fermentation Actinobacteria Culture Media Bioreactors Antineoplastic Agents Tryptophan Shikimic Acid Aquatic Organisms Gene Expression Profiling Biosynthetic Pathways The antitumor drug candidate, loonamycin, was isolated from Nocardiopsis flavescens NA01583 and exerts its antitumor activity by inhibiting topoisomerase I activity. However, the current production levels of loonamycin are insufficient for further research. To address this limitation, a liquid fermentation method was developed, optimizing both fermentation conditions and medium components. This optimization increased loonamycin production from 1 mg/mL on agar plate to 602.32 mg/mL in shake flask and ultimately 532.51 mg/mL in a 5-L bioreactor. Exogenous addition experiments revealed that sea salt and metal ions significantly inhibit loonamycin synthesis. Multi-temporal transcriptomic analyses indicated that the reduced expression levels of genes involved in the shikimate pathway and L-tryptophan biosynthesis pathway were the limiting factors for increased loonamycin yield. Overall, an effective fermentation method for rare marine actinomycetes was established, significantly enhancing loonamycin production and providing a foundation for the development of this novel antitumor prodrug. |
| title | Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens. |
| topic | Fermentation Actinobacteria Culture Media Bioreactors Antineoplastic Agents Tryptophan Shikimic Acid Aquatic Organisms Gene Expression Profiling Biosynthetic Pathways |
| url | https://pubmed.ncbi.nlm.nih.gov/40047177/ |