Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide.
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| Main Authors: | , , , |
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| Format: | Artículo científico |
| Language: | en |
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Archives of microbiology
2025
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| _version_ | 1868266234552778753 |
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| author | Wu, Jieer Han, Feng Yu, Yongxin Wang, Yongjie |
| author_facet | Wu, Jieer Han, Feng Yu, Yongxin Wang, Yongjie Wu, Jieer Han, Feng Yu, Yongxin Wang, Yongjie |
| collection | PubMed - marine biology |
| contents | Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide. Wu, Jieer Han, Feng Yu, Yongxin Wang, Yongjie Polysaccharides, Bacterial Pseudomonas Promoter Regions, Genetic Mutation Bacterial Proteins Multigene Family Arabinose Ostreidae Animals In our previous study, a strain of Pseudomonas composti ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 psl by substituting an exogenous arabinose-inducible promoter for the native promoter of the psl gene cluster. ODT-54 psl produced 50-70% more Psl EPS in FAB medium with 20 g/L arabinose induction compared to ODT-54, according to ELISA analyses. Furthermore, a 50% increase in binding to GII.6 P proteins was observed for ODT-54 psl. These findings confirmed the success of the construction of the ODT-54 Psl EPS overexpression strain. This work provides important experimental material and helps further explore the structure of Psl EPS from P. composti. |
| format | Artículo científico |
| id | pubmed_40053128 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Archives of microbiology |
| record_format | pubmed |
| spellingShingle | Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide. Wu, Jieer Han, Feng Yu, Yongxin Wang, Yongjie Polysaccharides, Bacterial Pseudomonas Promoter Regions, Genetic Mutation Bacterial Proteins Multigene Family Arabinose Ostreidae Animals Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide. Wu, Jieer Han, Feng Yu, Yongxin Wang, Yongjie Polysaccharides, Bacterial Pseudomonas Promoter Regions, Genetic Mutation Bacterial Proteins Multigene Family Arabinose Ostreidae Animals In our previous study, a strain of Pseudomonas composti ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 psl by substituting an exogenous arabinose-inducible promoter for the native promoter of the psl gene cluster. ODT-54 psl produced 50-70% more Psl EPS in FAB medium with 20 g/L arabinose induction compared to ODT-54, according to ELISA analyses. Furthermore, a 50% increase in binding to GII.6 P proteins was observed for ODT-54 psl. These findings confirmed the success of the construction of the ODT-54 Psl EPS overexpression strain. This work provides important experimental material and helps further explore the structure of Psl EPS from P. composti. |
| title | Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide. |
| topic | Polysaccharides, Bacterial Pseudomonas Promoter Regions, Genetic Mutation Bacterial Proteins Multigene Family Arabinose Ostreidae Animals |
| url | https://pubmed.ncbi.nlm.nih.gov/40053128/ |