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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of immunology (Baltimore, Md. : 1950)
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40073091/ |
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| _version_ | 1868266232029904897 |
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| author | Li, Kang Zhu, Yating Fang, Zhichao Geng, Ming Zhang, Jiansong Zheng, Yuying Cao, Yi Wei, Xiumei Yang, Jialong |
| author_facet | Li, Kang Zhu, Yating Fang, Zhichao Geng, Ming Zhang, Jiansong Zheng, Yuying Cao, Yi Wei, Xiumei Yang, Jialong Li, Kang Zhu, Yating Fang, Zhichao Geng, Ming Zhang, Jiansong Zheng, Yuying Cao, Yi Wei, Xiumei Yang, Jialong |
| collection | PubMed - marine biology |
| contents | Fish requires FasL to facilitate CD8+ T-cell function and antimicrobial immunity. Li, Kang Zhu, Yating Fang, Zhichao Geng, Ming Zhang, Jiansong Zheng, Yuying Cao, Yi Wei, Xiumei Yang, Jialong Animals Fas Ligand Protein CD8-Positive T-Lymphocytes Apoptosis Edwardsiella Enterobacteriaceae Infections Fish Diseases Cichlids Fish Proteins Although bony fish have CD8+ T cells, the mechanisms by which these early-evolved cytotoxic cells combat intracellular pathogens remain unclear. In the present study, using Nile tilapia as a model, we investigated the detailed function, mechanism, and evolutionary pattern concerning CD8+ T cells. By depleting CD8+ T cells, they are found essential in combating Edwardsiella piscicida infection. Using siRNA interference, we propose that unlike the strategy predominantly relying on perforin/granzyme in mammals, CD8+ T-cell effector function is mediated by both FasL and perforin/granzyme in fish. Upon E. piscicida infection, FasL is induced to express in CD8+ T cells; both recombinant FasL and adoptively transferred FasL+CD8+ T cells facilitate the apoptosis of target cells. Meanwhile, tilapia FasL also triggers the apoptosis of T cells to archive homeostasis. Since advances in mammals highlight the indispensable role of FasL in maintaining CD8+ T-cell homeostasis, rather than in effector function or anti-infective immunity, we therefore propose the unique dual function of FasL in executing effector function and maintaining homeostasis in fish. Mechanistically, tilapia T cells utilize mTORC1/c-Myc axis to regulate pathogen-induced FasL expression, which binds to Fas and activates caspase-8/caspase-3 pathway, mediating apoptosis in target cells and T cells themselves. This represents a novel mechanism underpinning CD8+ T-cell function in fish. Our findings demonstrate that CD8+ T cells reshaped the FasL-dependent strategy throughout evolution, thereby enhancing the precision and specificity of adaptive immunity. |
| format | Artículo científico |
| id | pubmed_40073091 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of immunology (Baltimore, Md. : 1950) |
| record_format | pubmed |
| spellingShingle | Fish requires FasL to facilitate CD8+ T-cell function and antimicrobial immunity. Li, Kang Zhu, Yating Fang, Zhichao Geng, Ming Zhang, Jiansong Zheng, Yuying Cao, Yi Wei, Xiumei Yang, Jialong Animals Fas Ligand Protein CD8-Positive T-Lymphocytes Apoptosis Edwardsiella Enterobacteriaceae Infections Fish Diseases Cichlids Fish Proteins Fish requires FasL to facilitate CD8+ T-cell function and antimicrobial immunity. Li, Kang Zhu, Yating Fang, Zhichao Geng, Ming Zhang, Jiansong Zheng, Yuying Cao, Yi Wei, Xiumei Yang, Jialong Animals Fas Ligand Protein CD8-Positive T-Lymphocytes Apoptosis Edwardsiella Enterobacteriaceae Infections Fish Diseases Cichlids Fish Proteins Although bony fish have CD8+ T cells, the mechanisms by which these early-evolved cytotoxic cells combat intracellular pathogens remain unclear. In the present study, using Nile tilapia as a model, we investigated the detailed function, mechanism, and evolutionary pattern concerning CD8+ T cells. By depleting CD8+ T cells, they are found essential in combating Edwardsiella piscicida infection. Using siRNA interference, we propose that unlike the strategy predominantly relying on perforin/granzyme in mammals, CD8+ T-cell effector function is mediated by both FasL and perforin/granzyme in fish. Upon E. piscicida infection, FasL is induced to express in CD8+ T cells; both recombinant FasL and adoptively transferred FasL+CD8+ T cells facilitate the apoptosis of target cells. Meanwhile, tilapia FasL also triggers the apoptosis of T cells to archive homeostasis. Since advances in mammals highlight the indispensable role of FasL in maintaining CD8+ T-cell homeostasis, rather than in effector function or anti-infective immunity, we therefore propose the unique dual function of FasL in executing effector function and maintaining homeostasis in fish. Mechanistically, tilapia T cells utilize mTORC1/c-Myc axis to regulate pathogen-induced FasL expression, which binds to Fas and activates caspase-8/caspase-3 pathway, mediating apoptosis in target cells and T cells themselves. This represents a novel mechanism underpinning CD8+ T-cell function in fish. Our findings demonstrate that CD8+ T cells reshaped the FasL-dependent strategy throughout evolution, thereby enhancing the precision and specificity of adaptive immunity. |
| title | Fish requires FasL to facilitate CD8+ T-cell function and antimicrobial immunity. |
| topic | Animals Fas Ligand Protein CD8-Positive T-Lymphocytes Apoptosis Edwardsiella Enterobacteriaceae Infections Fish Diseases Cichlids Fish Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/40073091/ |