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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of molecular sciences
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40076740/ |
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| _version_ | 1868266232023613442 |
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| author | Wang, Xiyue Wang, Miaoyunhuan Dong, Yuqing Yu, Shuqing Zhang, Shanshan Sun, Pinghua Wang, Lu Liu, Jibin Lin, Houwen Pan, Xinhui Li, Xiaobin |
| author_facet | Wang, Xiyue Wang, Miaoyunhuan Dong, Yuqing Yu, Shuqing Zhang, Shanshan Sun, Pinghua Wang, Lu Liu, Jibin Lin, Houwen Pan, Xinhui Li, Xiaobin Wang, Xiyue Wang, Miaoyunhuan Dong, Yuqing Yu, Shuqing Zhang, Shanshan Sun, Pinghua Wang, Lu Liu, Jibin Lin, Houwen Pan, Xinhui Li, Xiaobin |
| collection | PubMed - marine biology |
| contents | Antithrombotic Effect of L. Extract and Its Fractions via Regulating TLRs and the Downstream MAPKs and PI3K/AKT Signaling Pathways in Zebrafish. Wang, Xiyue Wang, Miaoyunhuan Dong, Yuqing Yu, Shuqing Zhang, Shanshan Sun, Pinghua Wang, Lu Liu, Jibin Lin, Houwen Pan, Xinhui Li, Xiaobin Animals Zebrafish Plant Extracts Proto-Oncogene Proteins c-akt Phosphatidylinositol 3-Kinases Toll-Like Receptors Signal Transduction Chenopodium album Fibrinolytic Agents Thrombosis MAP Kinase Signaling System Disease Models, Animal L., as a folkloric herb, is traditionally used to treat poisonous insect bites, vitiligo, and other ailments. However, its impact on thrombosis remains unknown. In this study, we discovered that the ethanol extract of exhibited a remarkable antithrombotic effect using a zebrafish thrombosis model for the first time. Activity evaluation showed that fraction CA-C could improve thrombus aggregation in the caudal vein, increase blood return in the heart, and alleviate the slowing of blood flow compared with those in the model group. Then, analysis by ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass (UPLC-Q-TOF-MS) identified 58 constituents of CA-C, with most of them belonging to flavonoids, alkaloids, and steroidal saponin components. Moreover, using a comprehensive strategy of network pharmacological analysis, transcriptomic assay, and RT-qPCR validation, we found that CA-C could mediate the TLR's signaling pathway and its downstream MAPKs and PI3K/AKT signaling pathways to exert an antithrombotic effect. This study broadens the clinical application of plant and provides new insight into the chemical profile, pharmacodynamics, and potential mechanisms of CA-C as candidate agents for treating thrombosis. |
| format | Artículo científico |
| id | pubmed_40076740 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | Antithrombotic Effect of L. Extract and Its Fractions via Regulating TLRs and the Downstream MAPKs and PI3K/AKT Signaling Pathways in Zebrafish. Wang, Xiyue Wang, Miaoyunhuan Dong, Yuqing Yu, Shuqing Zhang, Shanshan Sun, Pinghua Wang, Lu Liu, Jibin Lin, Houwen Pan, Xinhui Li, Xiaobin Animals Zebrafish Plant Extracts Proto-Oncogene Proteins c-akt Phosphatidylinositol 3-Kinases Toll-Like Receptors Signal Transduction Chenopodium album Fibrinolytic Agents Thrombosis MAP Kinase Signaling System Disease Models, Animal Antithrombotic Effect of L. Extract and Its Fractions via Regulating TLRs and the Downstream MAPKs and PI3K/AKT Signaling Pathways in Zebrafish. Wang, Xiyue Wang, Miaoyunhuan Dong, Yuqing Yu, Shuqing Zhang, Shanshan Sun, Pinghua Wang, Lu Liu, Jibin Lin, Houwen Pan, Xinhui Li, Xiaobin Animals Zebrafish Plant Extracts Proto-Oncogene Proteins c-akt Phosphatidylinositol 3-Kinases Toll-Like Receptors Signal Transduction Chenopodium album Fibrinolytic Agents Thrombosis MAP Kinase Signaling System Disease Models, Animal L., as a folkloric herb, is traditionally used to treat poisonous insect bites, vitiligo, and other ailments. However, its impact on thrombosis remains unknown. In this study, we discovered that the ethanol extract of exhibited a remarkable antithrombotic effect using a zebrafish thrombosis model for the first time. Activity evaluation showed that fraction CA-C could improve thrombus aggregation in the caudal vein, increase blood return in the heart, and alleviate the slowing of blood flow compared with those in the model group. Then, analysis by ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass (UPLC-Q-TOF-MS) identified 58 constituents of CA-C, with most of them belonging to flavonoids, alkaloids, and steroidal saponin components. Moreover, using a comprehensive strategy of network pharmacological analysis, transcriptomic assay, and RT-qPCR validation, we found that CA-C could mediate the TLR's signaling pathway and its downstream MAPKs and PI3K/AKT signaling pathways to exert an antithrombotic effect. This study broadens the clinical application of plant and provides new insight into the chemical profile, pharmacodynamics, and potential mechanisms of CA-C as candidate agents for treating thrombosis. |
| title | Antithrombotic Effect of L. Extract and Its Fractions via Regulating TLRs and the Downstream MAPKs and PI3K/AKT Signaling Pathways in Zebrafish. |
| topic | Animals Zebrafish Plant Extracts Proto-Oncogene Proteins c-akt Phosphatidylinositol 3-Kinases Toll-Like Receptors Signal Transduction Chenopodium album Fibrinolytic Agents Thrombosis MAP Kinase Signaling System Disease Models, Animal |
| url | https://pubmed.ncbi.nlm.nih.gov/40076740/ |