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| Main Authors: | , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Food chemistry
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40081063/ |
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| _version_ | 1868266232019419136 |
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| author | Naghdi, Shahab Rezaei, Masoud Tabarsa, Mehdi Abdollahi, Mehdi |
| author_facet | Naghdi, Shahab Rezaei, Masoud Tabarsa, Mehdi Abdollahi, Mehdi Naghdi, Shahab Rezaei, Masoud Tabarsa, Mehdi Abdollahi, Mehdi |
| collection | PubMed - marine biology |
| contents | Structure, functionality and bioactivity of sulfated polysaccharide extracted from rainbow trout byproducts: pH-shift method vs enzymatic hydrolysis. Naghdi, Shahab Rezaei, Masoud Tabarsa, Mehdi Abdollahi, Mehdi Oncorhynchus mykiss Polysaccharides Animals Hydrolysis Hydrogen-Ion Concentration Sulfates Antioxidants Waste Products Molecular Weight Chemical Fractionation Here, a novel method for sequentially extracting sulfated polysaccharides (SPs) from Oncorhynchus mykiss byproducts using alkaline/acid solubilization followed by isoelectric precipitation is compared with conventional enzymatic hydrolysis. Alkaline solubilization (SP-Alk) yielded SPs (2.46 %) comparable to the enzymatic method (SP-Enz, 2.77 %), while acidic solubilization (SP-Aci) yielded 1.96 %. SP-Alk showed comparable carbohydrate and sulfate content but lower protein than SP-Enz. Additionally, SP-Alk showed the highest monosaccharide content of rhamnose, mannose, glucose, and galactose. The extraction method affected the Molecular weight of SPs with SP-Enz having the lowest (44.95 kDa). Structural and thermal properties of the SPs were similar as revealed by FTIR/XRD and DSC, respectively. While SP-Enz exhibited slightly better antioxidant and functional properties (foaming, stability, emulsifying activity), SP-Alk showed a considerable performance with similar antimicrobial activity. Altogether, the pH-shift method can be a promising alternative for sequential extraction of SPs compared with enzymatic hydrolysis, avoiding enzymatic degradation of proteins. |
| format | Artículo científico |
| id | pubmed_40081063 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Food chemistry |
| record_format | pubmed |
| spellingShingle | Structure, functionality and bioactivity of sulfated polysaccharide extracted from rainbow trout byproducts: pH-shift method vs enzymatic hydrolysis. Naghdi, Shahab Rezaei, Masoud Tabarsa, Mehdi Abdollahi, Mehdi Oncorhynchus mykiss Polysaccharides Animals Hydrolysis Hydrogen-Ion Concentration Sulfates Antioxidants Waste Products Molecular Weight Chemical Fractionation Structure, functionality and bioactivity of sulfated polysaccharide extracted from rainbow trout byproducts: pH-shift method vs enzymatic hydrolysis. Naghdi, Shahab Rezaei, Masoud Tabarsa, Mehdi Abdollahi, Mehdi Oncorhynchus mykiss Polysaccharides Animals Hydrolysis Hydrogen-Ion Concentration Sulfates Antioxidants Waste Products Molecular Weight Chemical Fractionation Here, a novel method for sequentially extracting sulfated polysaccharides (SPs) from Oncorhynchus mykiss byproducts using alkaline/acid solubilization followed by isoelectric precipitation is compared with conventional enzymatic hydrolysis. Alkaline solubilization (SP-Alk) yielded SPs (2.46 %) comparable to the enzymatic method (SP-Enz, 2.77 %), while acidic solubilization (SP-Aci) yielded 1.96 %. SP-Alk showed comparable carbohydrate and sulfate content but lower protein than SP-Enz. Additionally, SP-Alk showed the highest monosaccharide content of rhamnose, mannose, glucose, and galactose. The extraction method affected the Molecular weight of SPs with SP-Enz having the lowest (44.95 kDa). Structural and thermal properties of the SPs were similar as revealed by FTIR/XRD and DSC, respectively. While SP-Enz exhibited slightly better antioxidant and functional properties (foaming, stability, emulsifying activity), SP-Alk showed a considerable performance with similar antimicrobial activity. Altogether, the pH-shift method can be a promising alternative for sequential extraction of SPs compared with enzymatic hydrolysis, avoiding enzymatic degradation of proteins. |
| title | Structure, functionality and bioactivity of sulfated polysaccharide extracted from rainbow trout byproducts: pH-shift method vs enzymatic hydrolysis. |
| topic | Oncorhynchus mykiss Polysaccharides Animals Hydrolysis Hydrogen-Ion Concentration Sulfates Antioxidants Waste Products Molecular Weight Chemical Fractionation |
| url | https://pubmed.ncbi.nlm.nih.gov/40081063/ |