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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40088845/ |
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| _version_ | 1868266232011030529 |
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| author | Zhuo, Jintao Zheng, Rikuan Luan, Zhendong Li, Lianfu Xi, Shichuan Du, Zengfeng He, Wanying Sun, Chaomin Zhang, Xin |
| author_facet | Zhuo, Jintao Zheng, Rikuan Luan, Zhendong Li, Lianfu Xi, Shichuan Du, Zengfeng He, Wanying Sun, Chaomin Zhang, Xin Zhuo, Jintao Zheng, Rikuan Luan, Zhendong Li, Lianfu Xi, Shichuan Du, Zengfeng He, Wanying Sun, Chaomin Zhang, Xin |
| collection | PubMed - marine biology |
| contents | Advancing anaerobic microbial studies with in situ Raman spectroscopy: Methanogenic archaea as a model. Zhuo, Jintao Zheng, Rikuan Luan, Zhendong Li, Lianfu Xi, Shichuan Du, Zengfeng He, Wanying Sun, Chaomin Zhang, Xin Spectrum Analysis, Raman Methane Anaerobiosis Archaea Carbon Dioxide Methanol Methanogenic archaea play a critical role in methane (CH) production and the global carbon cycle, yet accurately monitoring their gas metabolism under anaerobic conditions remains a technical challenge. In this study, we developed a Raman spectroscopy-based gas quantification model, achieving high-precision monitoring of CO-N-CH ternary gas mixtures over a temperature range of 12-52 °C. The model exhibited strong linear correlations between the Raman peak area ratios and gas molar ratios, which were further validated against gas chromatography, revealing no significant differences (p > 0.05). This confirms the reliability and accuracy of the approach.. Building upon this model, we conducted real-time monitoring of the gas metabolism of methylotrophic methanogenic archaea under anaerobic conditions. The results demonstrated that methanol concentration significantly influenced the gas production kinetics. At a methanol concentration of 10 μL/mL, the highest CH yield (59.97 %) was achieved, along with stable metabolic activity. In contrast, higher concentrations caused substrate saturation effects, leading to decreased metabolic efficiency. Furthermore, by integrating Raman spectroscopy with high-precision pressure monitoring, this study successfully achieved real-time molar quantification of CH and CO during methanogen cultivation. This approach provided detailed insights into gas production dynamics and substrate utilization patterns. Compared to traditional methods, this non-destructive, real-time monitoring platform offers a novel tool for anaerobic metabolism research and lays a solid foundation for applications in biogas optimization, industrial fermentation, and renewable energy development. |
| format | Artículo científico |
| id | pubmed_40088845 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy |
| record_format | pubmed |
| spellingShingle | Advancing anaerobic microbial studies with in situ Raman spectroscopy: Methanogenic archaea as a model. Zhuo, Jintao Zheng, Rikuan Luan, Zhendong Li, Lianfu Xi, Shichuan Du, Zengfeng He, Wanying Sun, Chaomin Zhang, Xin Spectrum Analysis, Raman Methane Anaerobiosis Archaea Carbon Dioxide Methanol Advancing anaerobic microbial studies with in situ Raman spectroscopy: Methanogenic archaea as a model. Zhuo, Jintao Zheng, Rikuan Luan, Zhendong Li, Lianfu Xi, Shichuan Du, Zengfeng He, Wanying Sun, Chaomin Zhang, Xin Spectrum Analysis, Raman Methane Anaerobiosis Archaea Carbon Dioxide Methanol Methanogenic archaea play a critical role in methane (CH) production and the global carbon cycle, yet accurately monitoring their gas metabolism under anaerobic conditions remains a technical challenge. In this study, we developed a Raman spectroscopy-based gas quantification model, achieving high-precision monitoring of CO-N-CH ternary gas mixtures over a temperature range of 12-52 °C. The model exhibited strong linear correlations between the Raman peak area ratios and gas molar ratios, which were further validated against gas chromatography, revealing no significant differences (p > 0.05). This confirms the reliability and accuracy of the approach.. Building upon this model, we conducted real-time monitoring of the gas metabolism of methylotrophic methanogenic archaea under anaerobic conditions. The results demonstrated that methanol concentration significantly influenced the gas production kinetics. At a methanol concentration of 10 μL/mL, the highest CH yield (59.97 %) was achieved, along with stable metabolic activity. In contrast, higher concentrations caused substrate saturation effects, leading to decreased metabolic efficiency. Furthermore, by integrating Raman spectroscopy with high-precision pressure monitoring, this study successfully achieved real-time molar quantification of CH and CO during methanogen cultivation. This approach provided detailed insights into gas production dynamics and substrate utilization patterns. Compared to traditional methods, this non-destructive, real-time monitoring platform offers a novel tool for anaerobic metabolism research and lays a solid foundation for applications in biogas optimization, industrial fermentation, and renewable energy development. |
| title | Advancing anaerobic microbial studies with in situ Raman spectroscopy: Methanogenic archaea as a model. |
| topic | Spectrum Analysis, Raman Methane Anaerobiosis Archaea Carbon Dioxide Methanol |
| url | https://pubmed.ncbi.nlm.nih.gov/40088845/ |