Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat.

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Main Authors: Kang, Min-Seok, Yu, Jae-Yon, Kim, Hyung-Seop, Dong, Ke, Srinivasan, Sathiyaraj, Lee, Sang-Seob
Format: Artículo científico
Language:en
Published: Current microbiology 2025
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author Kang, Min-Seok
Yu, Jae-Yon
Kim, Hyung-Seop
Dong, Ke
Srinivasan, Sathiyaraj
Lee, Sang-Seob
author_facet Kang, Min-Seok
Yu, Jae-Yon
Kim, Hyung-Seop
Dong, Ke
Srinivasan, Sathiyaraj
Lee, Sang-Seob
Kang, Min-Seok
Yu, Jae-Yon
Kim, Hyung-Seop
Dong, Ke
Srinivasan, Sathiyaraj
Lee, Sang-Seob
collection PubMed - marine biology
contents Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat. Kang, Min-Seok Yu, Jae-Yon Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob RNA, Ribosomal, 16S Phylogeny Base Composition Fatty Acids DNA, Bacterial Republic of Korea Bacterial Typing Techniques Nucleic Acid Hybridization Geologic Sediments Sequence Analysis, DNA Seawater A novel bacterial strain, designated DFM31, was isolated from a tidal flat in Gun-San, South Korea and characterized as a novel species within the genus Meridianimarinicoccus. Morphological and biochemical analyses indicated that strain DFM31 was a Gram-stain-negative, coccoid-shaped bacterium without flagella. The strain formed pale orange colonies on MA after 3 days of incubation at 30 °C. The strain was found to survive at 20-37 °C (optimum, 30 °C), pH 6-8 (optimum, pH 7), and 0.5-5% (w/v) NaCl (optimum, 3%). 16S rRNA gene similarity analysis indicated that the strain DFM31 was closely related to the genus Meridianimarinicoccus (95.1-97.5%). Biochemical tests showed that the strain was oxidase and catalase positive. The major fatty acids were Summed Feature 8 [C 7c/C 6c] (86.1%) and the major respiratory quinone was Q-10. The DNA G + C % of genomic DNA is 64.4. The values for Average amino acid (AAI), Average nucleotide identity (ANI), and DNA-DNA hybridization (DDH) compared with the related type strains in the genus Meridianimarinicoccus were 70.7-72%, 72.4-75.1%, and 18.7-19.2%, respectively. Based on its distinct phylogenetic, phenotypic, and biochemical characteristics, strain DFM31 is proposed to represent a novel species of the genus Meridianimarinicoccus, for which the name Meridianimarinicoccus marinus sp. nov. is proposed. The type strain is DFM31 (= KEMB 021938 = KCTC 8650 = JCM 37289).
format Artículo científico
id pubmed_40095045
institution PubMed
language en
publishDate 2025
publisher Current microbiology
record_format pubmed
spellingShingle Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat.
Kang, Min-Seok
Yu, Jae-Yon
Kim, Hyung-Seop
Dong, Ke
Srinivasan, Sathiyaraj
Lee, Sang-Seob
RNA, Ribosomal, 16S
Phylogeny
Base Composition
Fatty Acids
DNA, Bacterial
Republic of Korea
Bacterial Typing Techniques
Nucleic Acid Hybridization
Geologic Sediments
Sequence Analysis, DNA
Seawater
Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat. Kang, Min-Seok Yu, Jae-Yon Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob RNA, Ribosomal, 16S Phylogeny Base Composition Fatty Acids DNA, Bacterial Republic of Korea Bacterial Typing Techniques Nucleic Acid Hybridization Geologic Sediments Sequence Analysis, DNA Seawater A novel bacterial strain, designated DFM31, was isolated from a tidal flat in Gun-San, South Korea and characterized as a novel species within the genus Meridianimarinicoccus. Morphological and biochemical analyses indicated that strain DFM31 was a Gram-stain-negative, coccoid-shaped bacterium without flagella. The strain formed pale orange colonies on MA after 3 days of incubation at 30 °C. The strain was found to survive at 20-37 °C (optimum, 30 °C), pH 6-8 (optimum, pH 7), and 0.5-5% (w/v) NaCl (optimum, 3%). 16S rRNA gene similarity analysis indicated that the strain DFM31 was closely related to the genus Meridianimarinicoccus (95.1-97.5%). Biochemical tests showed that the strain was oxidase and catalase positive. The major fatty acids were Summed Feature 8 [C 7c/C 6c] (86.1%) and the major respiratory quinone was Q-10. The DNA G + C % of genomic DNA is 64.4. The values for Average amino acid (AAI), Average nucleotide identity (ANI), and DNA-DNA hybridization (DDH) compared with the related type strains in the genus Meridianimarinicoccus were 70.7-72%, 72.4-75.1%, and 18.7-19.2%, respectively. Based on its distinct phylogenetic, phenotypic, and biochemical characteristics, strain DFM31 is proposed to represent a novel species of the genus Meridianimarinicoccus, for which the name Meridianimarinicoccus marinus sp. nov. is proposed. The type strain is DFM31 (= KEMB 021938 = KCTC 8650 = JCM 37289).
title Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat.
topic RNA, Ribosomal, 16S
Phylogeny
Base Composition
Fatty Acids
DNA, Bacterial
Republic of Korea
Bacterial Typing Techniques
Nucleic Acid Hybridization
Geologic Sediments
Sequence Analysis, DNA
Seawater
url https://pubmed.ncbi.nlm.nih.gov/40095045/