Metagenome-Assembled Genomes for Oligotrophic Nitrifiers From a Mountainous Gravelbed Floodplain.
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| Format: | Artículo científico |
| Language: | en |
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Environmental microbiology
2025
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| author | Rasmussen, Anna N Tolar, Bradley B Bargar, John R Boye, Kristin Francis, Christopher A |
| author_facet | Rasmussen, Anna N Tolar, Bradley B Bargar, John R Boye, Kristin Francis, Christopher A Rasmussen, Anna N Tolar, Bradley B Bargar, John R Boye, Kristin Francis, Christopher A |
| collection | PubMed - marine biology |
| contents | Metagenome-Assembled Genomes for Oligotrophic Nitrifiers From a Mountainous Gravelbed Floodplain. Rasmussen, Anna N Tolar, Bradley B Bargar, John R Boye, Kristin Francis, Christopher A Archaea Nitrification Geologic Sediments Bacteria Ammonia Metagenome Nitrites Phylogeny Oxidation-Reduction Rivers Genome, Bacterial Riparian floodplains are important regions for biogeochemical cycling, including nitrogen. Here, we present MAGs from nitrifying microorganisms, including ammonia-oxidising archaea (AOA) and comammox bacteria from Slate River (SR) floodplain sediments (Crested Butte, CO, US). Additionally, we explore MAGs from potential nitrite-oxidising bacteria (NOB) from the Nitrospirales. AOA diversity in SR is lower than observed in other western US floodplain sediments and Nitrosotalea-like lineages such as the genus TA-20 are the dominant AOA. No ammonia-oxidising bacteria (AOB) MAGs were recovered. Microorganisms from the Palsa-1315 genus (clade B comammox) are the most abundant ammonia-oxidizers in SR floodplain sediments. Established NOB are conspicuously absent; however, we recovered MAGs from uncultured lineages of the NS-4 family (Nitrospirales) and Nitrospiraceae that we propose as putative NOB. Nitrite oxidation may be carried out by organisms sister to established Nitrospira NOB lineages based on the genomic content of uncultured Nitrospirales clades. Nitrifier MAGs recovered from SR floodplain sediments harbour genes for using alternative sources of ammonia, such as urea, cyanate, biuret, triuret and nitriles. The SR floodplain therefore appears to be a low ammonia flux environment that selects for oligotrophic nitrifiers. |
| format | Artículo científico |
| id | pubmed_40103293 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Environmental microbiology |
| record_format | pubmed |
| spellingShingle | Metagenome-Assembled Genomes for Oligotrophic Nitrifiers From a Mountainous Gravelbed Floodplain. Rasmussen, Anna N Tolar, Bradley B Bargar, John R Boye, Kristin Francis, Christopher A Archaea Nitrification Geologic Sediments Bacteria Ammonia Metagenome Nitrites Phylogeny Oxidation-Reduction Rivers Genome, Bacterial Metagenome-Assembled Genomes for Oligotrophic Nitrifiers From a Mountainous Gravelbed Floodplain. Rasmussen, Anna N Tolar, Bradley B Bargar, John R Boye, Kristin Francis, Christopher A Archaea Nitrification Geologic Sediments Bacteria Ammonia Metagenome Nitrites Phylogeny Oxidation-Reduction Rivers Genome, Bacterial Riparian floodplains are important regions for biogeochemical cycling, including nitrogen. Here, we present MAGs from nitrifying microorganisms, including ammonia-oxidising archaea (AOA) and comammox bacteria from Slate River (SR) floodplain sediments (Crested Butte, CO, US). Additionally, we explore MAGs from potential nitrite-oxidising bacteria (NOB) from the Nitrospirales. AOA diversity in SR is lower than observed in other western US floodplain sediments and Nitrosotalea-like lineages such as the genus TA-20 are the dominant AOA. No ammonia-oxidising bacteria (AOB) MAGs were recovered. Microorganisms from the Palsa-1315 genus (clade B comammox) are the most abundant ammonia-oxidizers in SR floodplain sediments. Established NOB are conspicuously absent; however, we recovered MAGs from uncultured lineages of the NS-4 family (Nitrospirales) and Nitrospiraceae that we propose as putative NOB. Nitrite oxidation may be carried out by organisms sister to established Nitrospira NOB lineages based on the genomic content of uncultured Nitrospirales clades. Nitrifier MAGs recovered from SR floodplain sediments harbour genes for using alternative sources of ammonia, such as urea, cyanate, biuret, triuret and nitriles. The SR floodplain therefore appears to be a low ammonia flux environment that selects for oligotrophic nitrifiers. |
| title | Metagenome-Assembled Genomes for Oligotrophic Nitrifiers From a Mountainous Gravelbed Floodplain. |
| topic | Archaea Nitrification Geologic Sediments Bacteria Ammonia Metagenome Nitrites Phylogeny Oxidation-Reduction Rivers Genome, Bacterial |
| url | https://pubmed.ncbi.nlm.nih.gov/40103293/ |