The genome assembly of the westslope cutthroat trout, Oncorhynchus lewisi, reveals interspecific chromosomal rearrangements with the rainbow trout, Oncorhynchus mykiss.

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Auteurs principaux: Flores, Anne-Marie, Christensen, Kris A, Godin, Theresa, Palti, Yniv, Campbell, Matthew R, Waldbieser, Geoffrey C, Simpson, Sheron A, Scheffler, Brian E, Smith, Seth R, Whiteley, Andrew R, Kovach, Ryan P, Luikart, Gordon, Boyer, Matthew C, Kardos, Marty, Relyea, Scott, Wells, Craig, Koop, Ben F
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Langue:en
Publié: G3 (Bethesda, Md.) 2025
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author Flores, Anne-Marie
Christensen, Kris A
Godin, Theresa
Palti, Yniv
Campbell, Matthew R
Waldbieser, Geoffrey C
Simpson, Sheron A
Scheffler, Brian E
Smith, Seth R
Whiteley, Andrew R
Kovach, Ryan P
Luikart, Gordon
Boyer, Matthew C
Kardos, Marty
Relyea, Scott
Wells, Craig
Koop, Ben F
author_facet Flores, Anne-Marie
Christensen, Kris A
Godin, Theresa
Palti, Yniv
Campbell, Matthew R
Waldbieser, Geoffrey C
Simpson, Sheron A
Scheffler, Brian E
Smith, Seth R
Whiteley, Andrew R
Kovach, Ryan P
Luikart, Gordon
Boyer, Matthew C
Kardos, Marty
Relyea, Scott
Wells, Craig
Koop, Ben F
Flores, Anne-Marie
Christensen, Kris A
Godin, Theresa
Palti, Yniv
Campbell, Matthew R
Waldbieser, Geoffrey C
Simpson, Sheron A
Scheffler, Brian E
Smith, Seth R
Whiteley, Andrew R
Kovach, Ryan P
Luikart, Gordon
Boyer, Matthew C
Kardos, Marty
Relyea, Scott
Wells, Craig
Koop, Ben F
collection PubMed - marine biology
contents The genome assembly of the westslope cutthroat trout, Oncorhynchus lewisi, reveals interspecific chromosomal rearrangements with the rainbow trout, Oncorhynchus mykiss. Flores, Anne-Marie Christensen, Kris A Godin, Theresa Palti, Yniv Campbell, Matthew R Waldbieser, Geoffrey C Simpson, Sheron A Scheffler, Brian E Smith, Seth R Whiteley, Andrew R Kovach, Ryan P Luikart, Gordon Boyer, Matthew C Kardos, Marty Relyea, Scott Wells, Craig Koop, Ben F Animals Oncorhynchus mykiss Genome Oncorhynchus Chromosome Mapping Hybridization, Genetic Haplotypes Genomics Gene Rearrangement Chromosomes Chromosome Aberrations Cutthroat trout (Oncorhynchus clarkii) are popular among anglers throughout their native range along the West Coast and interior of North America. As they colonized the interior of North America, cutthroat trout diverged into several genetically distinct groups. Many of these groups are now threatened by habitat destruction, hybridization with rainbow trout (Oncorhynchus mykiss), and competition from introduced species. These groups were previously classified as subspecies, but recent research suggests that they may represent distinct species. In this study, we produced a chromosomal-level genome assembly and a genetic map for one of the species in the cutthroat trout species complex, the westslope cutthroat trout (Oncorhynchus lewisi-formerly Oncorhynchus clarkii lewisi). We also constructed haplotype-resolved assemblies from a westslope cutthroat-rainbow trout F1 hybrid. We used the new genome assemblies to identify major interspecific chromosomal rearrangements between the 2 sister species, including fusions, fissions, and inversions. These genome assemblies and chromosome data provide valuable insights regarding genetic variation within cutthroat trout and in hybrids between rainbow and cutthroat trout.
format Artículo científico
id pubmed_40105693
institution PubMed
language en
publishDate 2025
publisher G3 (Bethesda, Md.)
record_format pubmed
spellingShingle The genome assembly of the westslope cutthroat trout, Oncorhynchus lewisi, reveals interspecific chromosomal rearrangements with the rainbow trout, Oncorhynchus mykiss.
Flores, Anne-Marie
Christensen, Kris A
Godin, Theresa
Palti, Yniv
Campbell, Matthew R
Waldbieser, Geoffrey C
Simpson, Sheron A
Scheffler, Brian E
Smith, Seth R
Whiteley, Andrew R
Kovach, Ryan P
Luikart, Gordon
Boyer, Matthew C
Kardos, Marty
Relyea, Scott
Wells, Craig
Koop, Ben F
Animals
Oncorhynchus mykiss
Genome
Oncorhynchus
Chromosome Mapping
Hybridization, Genetic
Haplotypes
Genomics
Gene Rearrangement
Chromosomes
Chromosome Aberrations
The genome assembly of the westslope cutthroat trout, Oncorhynchus lewisi, reveals interspecific chromosomal rearrangements with the rainbow trout, Oncorhynchus mykiss. Flores, Anne-Marie Christensen, Kris A Godin, Theresa Palti, Yniv Campbell, Matthew R Waldbieser, Geoffrey C Simpson, Sheron A Scheffler, Brian E Smith, Seth R Whiteley, Andrew R Kovach, Ryan P Luikart, Gordon Boyer, Matthew C Kardos, Marty Relyea, Scott Wells, Craig Koop, Ben F Animals Oncorhynchus mykiss Genome Oncorhynchus Chromosome Mapping Hybridization, Genetic Haplotypes Genomics Gene Rearrangement Chromosomes Chromosome Aberrations Cutthroat trout (Oncorhynchus clarkii) are popular among anglers throughout their native range along the West Coast and interior of North America. As they colonized the interior of North America, cutthroat trout diverged into several genetically distinct groups. Many of these groups are now threatened by habitat destruction, hybridization with rainbow trout (Oncorhynchus mykiss), and competition from introduced species. These groups were previously classified as subspecies, but recent research suggests that they may represent distinct species. In this study, we produced a chromosomal-level genome assembly and a genetic map for one of the species in the cutthroat trout species complex, the westslope cutthroat trout (Oncorhynchus lewisi-formerly Oncorhynchus clarkii lewisi). We also constructed haplotype-resolved assemblies from a westslope cutthroat-rainbow trout F1 hybrid. We used the new genome assemblies to identify major interspecific chromosomal rearrangements between the 2 sister species, including fusions, fissions, and inversions. These genome assemblies and chromosome data provide valuable insights regarding genetic variation within cutthroat trout and in hybrids between rainbow and cutthroat trout.
title The genome assembly of the westslope cutthroat trout, Oncorhynchus lewisi, reveals interspecific chromosomal rearrangements with the rainbow trout, Oncorhynchus mykiss.
topic Animals
Oncorhynchus mykiss
Genome
Oncorhynchus
Chromosome Mapping
Hybridization, Genetic
Haplotypes
Genomics
Gene Rearrangement
Chromosomes
Chromosome Aberrations
url https://pubmed.ncbi.nlm.nih.gov/40105693/