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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Development (Cambridge, England)
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40110772/ |
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| _version_ | 1868266228845379584 |
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| author | Yang, Wenyi Peng, Mingjian Wang, Youquan Zhang, Xiaowen Li, Wei Zhai, Xue Wu, Zhichao Hu, Peng Chen, Liangbiao |
| author_facet | Yang, Wenyi Peng, Mingjian Wang, Youquan Zhang, Xiaowen Li, Wei Zhai, Xue Wu, Zhichao Hu, Peng Chen, Liangbiao Yang, Wenyi Peng, Mingjian Wang, Youquan Zhang, Xiaowen Li, Wei Zhai, Xue Wu, Zhichao Hu, Peng Chen, Liangbiao |
| collection | PubMed - marine biology |
| contents | Deletion of hepcidin disrupts iron homeostasis and hematopoiesis in zebrafish embryogenesis. Yang, Wenyi Peng, Mingjian Wang, Youquan Zhang, Xiaowen Li, Wei Zhai, Xue Wu, Zhichao Hu, Peng Chen, Liangbiao Animals Zebrafish Hepcidins Hematopoiesis Iron Homeostasis Embryonic Development Zebrafish Proteins CRISPR-Cas Systems Gene Expression Regulation, Developmental Ferroptosis Gene Deletion Embryo, Nonmammalian Gene Knockout Techniques GATA1 Transcription Factor Iron is essential for cell growth and hematopoiesis, which is regulated by hepcidin (hamp). However, the role of hamp in zebrafish hematopoiesis remains unclear. Here, we have created a stable hamp knockout zebrafish model using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 system (CRISPR/Cas9 system). Our study revealed that hamp deletion led to maternal iron overload in embryos, significantly downregulating hemoglobin genes and reducing hemoglobin content. Single-cell RNA sequencing identified abnormal expression patterns in blood progenitor cells, with a specific progenitor subtype showing increased ferroptosis and delayed development. By crossing hamp knockout zebrafish with a gata1+ line (blood cells labeled fish line), we confirmed ferroptosis in blood progenitor cells. These findings underscore the crucial role of hamp in iron regulation and hematopoiesis, offering novel insights into developmental biology and potential therapeutic targets for blood disorders. |
| format | Artículo científico |
| id | pubmed_40110772 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Development (Cambridge, England) |
| record_format | pubmed |
| spellingShingle | Deletion of hepcidin disrupts iron homeostasis and hematopoiesis in zebrafish embryogenesis. Yang, Wenyi Peng, Mingjian Wang, Youquan Zhang, Xiaowen Li, Wei Zhai, Xue Wu, Zhichao Hu, Peng Chen, Liangbiao Animals Zebrafish Hepcidins Hematopoiesis Iron Homeostasis Embryonic Development Zebrafish Proteins CRISPR-Cas Systems Gene Expression Regulation, Developmental Ferroptosis Gene Deletion Embryo, Nonmammalian Gene Knockout Techniques GATA1 Transcription Factor Deletion of hepcidin disrupts iron homeostasis and hematopoiesis in zebrafish embryogenesis. Yang, Wenyi Peng, Mingjian Wang, Youquan Zhang, Xiaowen Li, Wei Zhai, Xue Wu, Zhichao Hu, Peng Chen, Liangbiao Animals Zebrafish Hepcidins Hematopoiesis Iron Homeostasis Embryonic Development Zebrafish Proteins CRISPR-Cas Systems Gene Expression Regulation, Developmental Ferroptosis Gene Deletion Embryo, Nonmammalian Gene Knockout Techniques GATA1 Transcription Factor Iron is essential for cell growth and hematopoiesis, which is regulated by hepcidin (hamp). However, the role of hamp in zebrafish hematopoiesis remains unclear. Here, we have created a stable hamp knockout zebrafish model using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 system (CRISPR/Cas9 system). Our study revealed that hamp deletion led to maternal iron overload in embryos, significantly downregulating hemoglobin genes and reducing hemoglobin content. Single-cell RNA sequencing identified abnormal expression patterns in blood progenitor cells, with a specific progenitor subtype showing increased ferroptosis and delayed development. By crossing hamp knockout zebrafish with a gata1+ line (blood cells labeled fish line), we confirmed ferroptosis in blood progenitor cells. These findings underscore the crucial role of hamp in iron regulation and hematopoiesis, offering novel insights into developmental biology and potential therapeutic targets for blood disorders. |
| title | Deletion of hepcidin disrupts iron homeostasis and hematopoiesis in zebrafish embryogenesis. |
| topic | Animals Zebrafish Hepcidins Hematopoiesis Iron Homeostasis Embryonic Development Zebrafish Proteins CRISPR-Cas Systems Gene Expression Regulation, Developmental Ferroptosis Gene Deletion Embryo, Nonmammalian Gene Knockout Techniques GATA1 Transcription Factor |
| url | https://pubmed.ncbi.nlm.nih.gov/40110772/ |