Ulvan derived from Ulva lactuca suppresses hepatocellular carcinoma cell proliferation through miR-542-3p-mediated downregulation of SLC35F6.
Fuente:
PubMed
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of biological macromolecules
2025
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266228829650944 |
|---|---|
| author | Qiu, Yinghui Xu, Jingxiang Liao, Wei Yang, Shuxin Wen, Yuxi Farag, Mohamed A Zheng, Lingjun Zhao, Chao |
| author_facet | Qiu, Yinghui Xu, Jingxiang Liao, Wei Yang, Shuxin Wen, Yuxi Farag, Mohamed A Zheng, Lingjun Zhao, Chao Qiu, Yinghui Xu, Jingxiang Liao, Wei Yang, Shuxin Wen, Yuxi Farag, Mohamed A Zheng, Lingjun Zhao, Chao |
| collection | PubMed - marine biology |
| contents | Ulvan derived from Ulva lactuca suppresses hepatocellular carcinoma cell proliferation through miR-542-3p-mediated downregulation of SLC35F6. Qiu, Yinghui Xu, Jingxiang Liao, Wei Yang, Shuxin Wen, Yuxi Farag, Mohamed A Zheng, Lingjun Zhao, Chao MicroRNAs Humans Carcinoma, Hepatocellular Liver Neoplasms Cell Proliferation Gene Expression Regulation, Neoplastic Ulva Polysaccharides Tumor Suppressor Protein p53 Cell Line, Tumor Down-Regulation Hep G2 Cells Edible Seaweeds Hepatocellular carcinoma (HCC) therapy still presents significant challenges, with a critical need for novel molecular targets and effective natural compound-based therapies. Despite its known oncogenic potential in other cancers, the role of SLC35F6 in HCC has not been previously reported, leaving a gap in our understanding of its function and therapeutic relevance. Here, we demonstrate that SLC35F6 is overexpressed in HCC and is associated with poor prognosis. Ulva lactuca polysaccharide (ULP), a natural extract with known antitumor properties, exerts its effects by upregulating miR-542-3p, which in turn inhibits SLC35F6 expression and significantly increases TP53 protein levels. Furthermore, TP53 is positively regulated by miR-542-3p, and our results indicate that SLC35F6 is a target gene of miR-542-3p. Knockdown of SLC35F6 in H22 and HepG2 cells markedly reduced cell growth while elevating TP53 expression, supporting SLC35F6 as a key regulatory factor in the miR-542-3p/TP53 axis. While this study did not confirm direct mutual regulation between SLC35F6 and TP53, our findings provide evidence that targeting SLC35F6 can suppress HCC progression. Collectively, these results identify SLC35F6 as a potential therapeutic target for HCC and provide mechanistic insights into its regulation through the miR-542-3p/SLC35F6/TP53 axis. |
| format | Artículo científico |
| id | pubmed_40118430 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Ulvan derived from Ulva lactuca suppresses hepatocellular carcinoma cell proliferation through miR-542-3p-mediated downregulation of SLC35F6. Qiu, Yinghui Xu, Jingxiang Liao, Wei Yang, Shuxin Wen, Yuxi Farag, Mohamed A Zheng, Lingjun Zhao, Chao MicroRNAs Humans Carcinoma, Hepatocellular Liver Neoplasms Cell Proliferation Gene Expression Regulation, Neoplastic Ulva Polysaccharides Tumor Suppressor Protein p53 Cell Line, Tumor Down-Regulation Hep G2 Cells Edible Seaweeds Ulvan derived from Ulva lactuca suppresses hepatocellular carcinoma cell proliferation through miR-542-3p-mediated downregulation of SLC35F6. Qiu, Yinghui Xu, Jingxiang Liao, Wei Yang, Shuxin Wen, Yuxi Farag, Mohamed A Zheng, Lingjun Zhao, Chao MicroRNAs Humans Carcinoma, Hepatocellular Liver Neoplasms Cell Proliferation Gene Expression Regulation, Neoplastic Ulva Polysaccharides Tumor Suppressor Protein p53 Cell Line, Tumor Down-Regulation Hep G2 Cells Edible Seaweeds Hepatocellular carcinoma (HCC) therapy still presents significant challenges, with a critical need for novel molecular targets and effective natural compound-based therapies. Despite its known oncogenic potential in other cancers, the role of SLC35F6 in HCC has not been previously reported, leaving a gap in our understanding of its function and therapeutic relevance. Here, we demonstrate that SLC35F6 is overexpressed in HCC and is associated with poor prognosis. Ulva lactuca polysaccharide (ULP), a natural extract with known antitumor properties, exerts its effects by upregulating miR-542-3p, which in turn inhibits SLC35F6 expression and significantly increases TP53 protein levels. Furthermore, TP53 is positively regulated by miR-542-3p, and our results indicate that SLC35F6 is a target gene of miR-542-3p. Knockdown of SLC35F6 in H22 and HepG2 cells markedly reduced cell growth while elevating TP53 expression, supporting SLC35F6 as a key regulatory factor in the miR-542-3p/TP53 axis. While this study did not confirm direct mutual regulation between SLC35F6 and TP53, our findings provide evidence that targeting SLC35F6 can suppress HCC progression. Collectively, these results identify SLC35F6 as a potential therapeutic target for HCC and provide mechanistic insights into its regulation through the miR-542-3p/SLC35F6/TP53 axis. |
| title | Ulvan derived from Ulva lactuca suppresses hepatocellular carcinoma cell proliferation through miR-542-3p-mediated downregulation of SLC35F6. |
| topic | MicroRNAs Humans Carcinoma, Hepatocellular Liver Neoplasms Cell Proliferation Gene Expression Regulation, Neoplastic Ulva Polysaccharides Tumor Suppressor Protein p53 Cell Line, Tumor Down-Regulation Hep G2 Cells Edible Seaweeds |
| url | https://pubmed.ncbi.nlm.nih.gov/40118430/ |