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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Microorganisms
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40142530/ |
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| _version_ | 1868266226123276290 |
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| author | Kurilenko, Valeriya Bystritskaya, Evgenia Otstavnykh, Nadezhda Velansky, Peter Lichmanuk, Darina Savicheva, Yulia Romanenko, Lyudmila Isaeva, Marina |
| author_facet | Kurilenko, Valeriya Bystritskaya, Evgenia Otstavnykh, Nadezhda Velansky, Peter Lichmanuk, Darina Savicheva, Yulia Romanenko, Lyudmila Isaeva, Marina Kurilenko, Valeriya Bystritskaya, Evgenia Otstavnykh, Nadezhda Velansky, Peter Lichmanuk, Darina Savicheva, Yulia Romanenko, Lyudmila Isaeva, Marina |
| collection | PubMed - marine biology |
| contents | Description and Genome-Based Analysis of sp. nov., a New Species of the Mediterranei Clade Isolated from a Marine Polychaete. Kurilenko, Valeriya Bystritskaya, Evgenia Otstavnykh, Nadezhda Velansky, Peter Lichmanuk, Darina Savicheva, Yulia Romanenko, Lyudmila Isaeva, Marina Two novel strains, CB1-14 and CB2-10, were isolated from the marine polychaetes from the Sea of Japan. Phylogenetic analysis based on the 16S rRNA sequences revealed that the two strains belong to the genus , sharing 98.96% identity with CN 83. MLSA using five protein-coding genes (, and ) showed that CB1-14 and CB2-10 are closely related to the members of the Mediterranei clade, namely CECT 621, 3062, CECT 8203, CGMCC 1.7062, CAIM 1455, and CAIM 1454. Based on both MLST neighbor-net phylogenetic network and phylogenomic tree results, they fell into the subclade formed by CAIM 1455 and CAIM 1454. Both new strains CB1-14 and CB2-10 showed the highest ANI/AAI values of 91.3%/92.7% with CAIM 1454 and 90.3%/93.1% with CAIM 1455. The dDDH values between strain CB1-14 and the members of the Mediterranei clade ranged from 20.9% to 45.7%. Major fatty acids were C9, C7, and C9, followed by C and C7. The genome of CB1-14 is 5,591,686 bp in size, with DNA G+C content of 46.1%. It consists of two circular chromosomes (3,497,892 and 1,804,652 bp) and one plasmid (241,015 bp) and comprises 4782 protein-coding genes and 10 operons. The CB1-14 and CB2-10 genomes were enriched in CAZyme-encoding genes of the following families: GH1, GH3, GH13, GH23, GH43, GH94, PL17, and CE4, indicating the potential to catabolize alginate, xylan, and chitin, common polysaccharides in marine ecosystems. Based on the combined phylogenomic analyses and phenotypic properties, a new species, sp. nov., is proposed, with CB1-14 = (KMM 8419 = KCTC 92790) as the type strain. |
| format | Artículo científico |
| id | pubmed_40142530 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Description and Genome-Based Analysis of sp. nov., a New Species of the Mediterranei Clade Isolated from a Marine Polychaete. Kurilenko, Valeriya Bystritskaya, Evgenia Otstavnykh, Nadezhda Velansky, Peter Lichmanuk, Darina Savicheva, Yulia Romanenko, Lyudmila Isaeva, Marina Description and Genome-Based Analysis of sp. nov., a New Species of the Mediterranei Clade Isolated from a Marine Polychaete. Kurilenko, Valeriya Bystritskaya, Evgenia Otstavnykh, Nadezhda Velansky, Peter Lichmanuk, Darina Savicheva, Yulia Romanenko, Lyudmila Isaeva, Marina Two novel strains, CB1-14 and CB2-10, were isolated from the marine polychaetes from the Sea of Japan. Phylogenetic analysis based on the 16S rRNA sequences revealed that the two strains belong to the genus , sharing 98.96% identity with CN 83. MLSA using five protein-coding genes (, and ) showed that CB1-14 and CB2-10 are closely related to the members of the Mediterranei clade, namely CECT 621, 3062, CECT 8203, CGMCC 1.7062, CAIM 1455, and CAIM 1454. Based on both MLST neighbor-net phylogenetic network and phylogenomic tree results, they fell into the subclade formed by CAIM 1455 and CAIM 1454. Both new strains CB1-14 and CB2-10 showed the highest ANI/AAI values of 91.3%/92.7% with CAIM 1454 and 90.3%/93.1% with CAIM 1455. The dDDH values between strain CB1-14 and the members of the Mediterranei clade ranged from 20.9% to 45.7%. Major fatty acids were C9, C7, and C9, followed by C and C7. The genome of CB1-14 is 5,591,686 bp in size, with DNA G+C content of 46.1%. It consists of two circular chromosomes (3,497,892 and 1,804,652 bp) and one plasmid (241,015 bp) and comprises 4782 protein-coding genes and 10 operons. The CB1-14 and CB2-10 genomes were enriched in CAZyme-encoding genes of the following families: GH1, GH3, GH13, GH23, GH43, GH94, PL17, and CE4, indicating the potential to catabolize alginate, xylan, and chitin, common polysaccharides in marine ecosystems. Based on the combined phylogenomic analyses and phenotypic properties, a new species, sp. nov., is proposed, with CB1-14 = (KMM 8419 = KCTC 92790) as the type strain. |
| title | Description and Genome-Based Analysis of sp. nov., a New Species of the Mediterranei Clade Isolated from a Marine Polychaete. |
| url | https://pubmed.ncbi.nlm.nih.gov/40142530/ |