Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of biological macromolecules
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40164253/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266223133786113 |
|---|---|
| author | Wu, Yanduo Li, Jun Xiao, Zhizhong Li, Wensheng Xiao, Yongshuang |
| author_facet | Wu, Yanduo Li, Jun Xiao, Zhizhong Li, Wensheng Xiao, Yongshuang Wu, Yanduo Li, Jun Xiao, Zhizhong Li, Wensheng Xiao, Yongshuang |
| collection | PubMed - marine biology |
| contents | Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus). Wu, Yanduo Li, Jun Xiao, Zhizhong Li, Wensheng Xiao, Yongshuang Animals Receptors, G-Protein-Coupled Genomics Feeding Behavior Receptors, Odorant Phylogeny Multigene Family Gene Expression Profiling Perciformes Fishes The Spotted knifejaw was previously considered a predominantly carnivorous species, but emerging evidence suggests that it exhibits omnivorous characteristics. However, the mechanisms underpinning its dietary remain poorly understood; Behavioral experiments have demonstrated that the fish shows preferences for both carnivorous and herbivorous foods and olfactory dysfunction was found to significantly reduced the responsiveness to food stimuli. Anatomical and histological analyses revealed a well-developed olfactory system, with increases in the number of olfactory lamellae and epithelial cells at 50 and 120 dph, which were consistent with spatio-temporal gene expression patterns. Genomic analyses identified ORs within the GPCR family, including 164 MORs, 77 TAARs, 4 V1Rs, and 7 V2Rs. Notably, a significant expansion of the δ subtype of MORs was observed suggesting a role in omnivorous adaptation. Transcriptomic WGCNA revealed the pathways related to protein digestion and absorption, pancreatic secretion, olfactory transduction, and gastric acid secretion. It is hypothesized that the expansion of TAAR13c is related to the carnivorous nature of O. punctatus. In situ hybridization confirmed the expression of key ORs in olfactory epithelial cells, sensory neurons, and intestinal endocrine cells, and functional validation of ORs using the dual luciferase assay, providing new insights into the molecular mechanisms governing omnivory in the Spotted knifejaw. |
| format | Artículo científico |
| id | pubmed_40164253 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus). Wu, Yanduo Li, Jun Xiao, Zhizhong Li, Wensheng Xiao, Yongshuang Animals Receptors, G-Protein-Coupled Genomics Feeding Behavior Receptors, Odorant Phylogeny Multigene Family Gene Expression Profiling Perciformes Fishes Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus). Wu, Yanduo Li, Jun Xiao, Zhizhong Li, Wensheng Xiao, Yongshuang Animals Receptors, G-Protein-Coupled Genomics Feeding Behavior Receptors, Odorant Phylogeny Multigene Family Gene Expression Profiling Perciformes Fishes The Spotted knifejaw was previously considered a predominantly carnivorous species, but emerging evidence suggests that it exhibits omnivorous characteristics. However, the mechanisms underpinning its dietary remain poorly understood; Behavioral experiments have demonstrated that the fish shows preferences for both carnivorous and herbivorous foods and olfactory dysfunction was found to significantly reduced the responsiveness to food stimuli. Anatomical and histological analyses revealed a well-developed olfactory system, with increases in the number of olfactory lamellae and epithelial cells at 50 and 120 dph, which were consistent with spatio-temporal gene expression patterns. Genomic analyses identified ORs within the GPCR family, including 164 MORs, 77 TAARs, 4 V1Rs, and 7 V2Rs. Notably, a significant expansion of the δ subtype of MORs was observed suggesting a role in omnivorous adaptation. Transcriptomic WGCNA revealed the pathways related to protein digestion and absorption, pancreatic secretion, olfactory transduction, and gastric acid secretion. It is hypothesized that the expansion of TAAR13c is related to the carnivorous nature of O. punctatus. In situ hybridization confirmed the expression of key ORs in olfactory epithelial cells, sensory neurons, and intestinal endocrine cells, and functional validation of ORs using the dual luciferase assay, providing new insights into the molecular mechanisms governing omnivory in the Spotted knifejaw. |
| title | Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus). |
| topic | Animals Receptors, G-Protein-Coupled Genomics Feeding Behavior Receptors, Odorant Phylogeny Multigene Family Gene Expression Profiling Perciformes Fishes |
| url | https://pubmed.ncbi.nlm.nih.gov/40164253/ |