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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40180204/ |
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| _version_ | 1868266221023002624 |
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| author | Yin, Jiayue Wen, Shuyang Huang, Yunyi Zhang, Xin Wang, Yanhong Hu, Chaoqun Li, Huo Chen, Guoqiang Zhu, Jingxuan Luo, Peng |
| author_facet | Yin, Jiayue Wen, Shuyang Huang, Yunyi Zhang, Xin Wang, Yanhong Hu, Chaoqun Li, Huo Chen, Guoqiang Zhu, Jingxuan Luo, Peng Yin, Jiayue Wen, Shuyang Huang, Yunyi Zhang, Xin Wang, Yanhong Hu, Chaoqun Li, Huo Chen, Guoqiang Zhu, Jingxuan Luo, Peng |
| collection | PubMed - marine biology |
| contents | Correlation between variations in promoter region of LvITGβ gene and anti-infection trait of shrimp, Litopenaeus vannamei, against a microsporidium, Ecytonucleospora hepatopenaei. Yin, Jiayue Wen, Shuyang Huang, Yunyi Zhang, Xin Wang, Yanhong Hu, Chaoqun Li, Huo Chen, Guoqiang Zhu, Jingxuan Luo, Peng Animals Penaeidae Arthropod Proteins Promoter Regions, Genetic Disease Resistance Immunity, Innate Enterocytozoon Genetic Variation The growth and survival of shrimp Litopenaeus vannamei is significantly impacted by Ecytonucleospora hepatopenaei (EHP) infection, which has become one of the major threats to shrimp farming. Integrins (ITGs), functioning as cell adhesion receptors, play a critical role in the immune response of shrimps to EHP invasion. In this study, correlation between genetic variations in the promoter region of LvITGβ coding integrin β subunit and EHP-resistant trait of L. vannamei were deeply analyzed. Four experimental groups (ZJ01, ZJ02, ZJ03, and ZJ04) containing individuals with extreme EHP-resistant performances were selected to screen candidate genetic markers associated with EHP resistance, and subsequently the candidate genetic markers were further verified through using a VAL group. The relative expression of LvITGβ was significantly up-regulated in EHP-susceptible individuals reflected by higher EHP load. Chi-square (χ) tests of individuals with extreme EHP-resistant performances revealed significant differences in allele distribution at loci, g.-722, g.-711, g.-294, and g.-268. Linkage disequilibrium analysis showed significant linkage between g.-294 and g.-268 (R = 0.33). A combined TT/AA genotype at the two loci considerably strongly associated with EHP resistance in four experimental groups, which was further verified in the VAL group. Therefore, this combined genotype was determined as a prominent SNP marker, and it has a huge application potential in genetic breeding of shrimp L. vannamei aiming at enhancing EHP resistance. These findings provide valuable insights for selecting shrimp individuals and breeding populations for enhanced resistance. |
| format | Artículo científico |
| id | pubmed_40180204 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Correlation between variations in promoter region of LvITGβ gene and anti-infection trait of shrimp, Litopenaeus vannamei, against a microsporidium, Ecytonucleospora hepatopenaei. Yin, Jiayue Wen, Shuyang Huang, Yunyi Zhang, Xin Wang, Yanhong Hu, Chaoqun Li, Huo Chen, Guoqiang Zhu, Jingxuan Luo, Peng Animals Penaeidae Arthropod Proteins Promoter Regions, Genetic Disease Resistance Immunity, Innate Enterocytozoon Genetic Variation Correlation between variations in promoter region of LvITGβ gene and anti-infection trait of shrimp, Litopenaeus vannamei, against a microsporidium, Ecytonucleospora hepatopenaei. Yin, Jiayue Wen, Shuyang Huang, Yunyi Zhang, Xin Wang, Yanhong Hu, Chaoqun Li, Huo Chen, Guoqiang Zhu, Jingxuan Luo, Peng Animals Penaeidae Arthropod Proteins Promoter Regions, Genetic Disease Resistance Immunity, Innate Enterocytozoon Genetic Variation The growth and survival of shrimp Litopenaeus vannamei is significantly impacted by Ecytonucleospora hepatopenaei (EHP) infection, which has become one of the major threats to shrimp farming. Integrins (ITGs), functioning as cell adhesion receptors, play a critical role in the immune response of shrimps to EHP invasion. In this study, correlation between genetic variations in the promoter region of LvITGβ coding integrin β subunit and EHP-resistant trait of L. vannamei were deeply analyzed. Four experimental groups (ZJ01, ZJ02, ZJ03, and ZJ04) containing individuals with extreme EHP-resistant performances were selected to screen candidate genetic markers associated with EHP resistance, and subsequently the candidate genetic markers were further verified through using a VAL group. The relative expression of LvITGβ was significantly up-regulated in EHP-susceptible individuals reflected by higher EHP load. Chi-square (χ) tests of individuals with extreme EHP-resistant performances revealed significant differences in allele distribution at loci, g.-722, g.-711, g.-294, and g.-268. Linkage disequilibrium analysis showed significant linkage between g.-294 and g.-268 (R = 0.33). A combined TT/AA genotype at the two loci considerably strongly associated with EHP resistance in four experimental groups, which was further verified in the VAL group. Therefore, this combined genotype was determined as a prominent SNP marker, and it has a huge application potential in genetic breeding of shrimp L. vannamei aiming at enhancing EHP resistance. These findings provide valuable insights for selecting shrimp individuals and breeding populations for enhanced resistance. |
| title | Correlation between variations in promoter region of LvITGβ gene and anti-infection trait of shrimp, Litopenaeus vannamei, against a microsporidium, Ecytonucleospora hepatopenaei. |
| topic | Animals Penaeidae Arthropod Proteins Promoter Regions, Genetic Disease Resistance Immunity, Innate Enterocytozoon Genetic Variation |
| url | https://pubmed.ncbi.nlm.nih.gov/40180204/ |