Functional Characterization of Luciferase in a Brittle Star Indicates Parallel Evolution Influenced by Genomic Availability of Haloalkane Dehalogenase.
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| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Molecular biology and evolution
2025
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| _version_ | 1868266221020905472 |
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| author | Lau, Emily S Majerova, Marika Hensley, Nicholai M Mukherjee, Arnab Vasina, Michal Pluskal, Daniel Damborsky, Jiri Prokop, Zbynek Delroisse, Jérôme Bayaert, Wendy-Shirley Parey, Elise Oliveri, Paola Marlétaz, Ferdinand Marek, Martin Oakley, Todd H |
| author_facet | Lau, Emily S Majerova, Marika Hensley, Nicholai M Mukherjee, Arnab Vasina, Michal Pluskal, Daniel Damborsky, Jiri Prokop, Zbynek Delroisse, Jérôme Bayaert, Wendy-Shirley Parey, Elise Oliveri, Paola Marlétaz, Ferdinand Marek, Martin Oakley, Todd H Lau, Emily S Majerova, Marika Hensley, Nicholai M Mukherjee, Arnab Vasina, Michal Pluskal, Daniel Damborsky, Jiri Prokop, Zbynek Delroisse, Jérôme Bayaert, Wendy-Shirley Parey, Elise Oliveri, Paola Marlétaz, Ferdinand Marek, Martin Oakley, Todd H |
| collection | PubMed - marine biology |
| contents | Functional Characterization of Luciferase in a Brittle Star Indicates Parallel Evolution Influenced by Genomic Availability of Haloalkane Dehalogenase. Lau, Emily S Majerova, Marika Hensley, Nicholai M Mukherjee, Arnab Vasina, Michal Pluskal, Daniel Damborsky, Jiri Prokop, Zbynek Delroisse, Jérôme Bayaert, Wendy-Shirley Parey, Elise Oliveri, Paola Marlétaz, Ferdinand Marek, Martin Oakley, Todd H Echinodermata Animals Hydrolases Luciferases Genomics Evolution, Molecular Genome Kinetics Biocatalysis Phylogeny Determining why convergent traits use distinct versus shared genetic components is crucial for understanding how evolutionary processes generate and sustain biodiversity. However, the factors dictating the genetic underpinnings of convergent traits remain incompletely understood. Here, we use heterologous protein expression, biochemical assays, and phylogenetic analyses to confirm the origin of a luciferase gene from haloalkane dehalogenases in the brittle star Amphiura filiformis. Through database searches and gene tree analyses, we also show a complex pattern of the presence and absence of haloalkane dehalogenases across organismal genomes. These results first confirm parallel evolution across a vast phylogenetic distance, because octocorals like Renilla also use luciferase derived from haloalkane dehalogenases. This parallel evolution is surprising, even though previously hypothesized, because many organisms that also use coelenterazine as the bioluminescence substrate evolved completely distinct luciferases. The inability to detect haloalkane dehalogenases in the genomes of several bioluminescent groups suggests that the distribution of this gene family influences its recruitment as a luciferase. Together, our findings highlight how biochemical function and genomic availability help determine whether distinct or shared genetic components are used during the convergent evolution of traits like bioluminescence. |
| format | Artículo científico |
| id | pubmed_40181585 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Molecular biology and evolution |
| record_format | pubmed |
| spellingShingle | Functional Characterization of Luciferase in a Brittle Star Indicates Parallel Evolution Influenced by Genomic Availability of Haloalkane Dehalogenase. Lau, Emily S Majerova, Marika Hensley, Nicholai M Mukherjee, Arnab Vasina, Michal Pluskal, Daniel Damborsky, Jiri Prokop, Zbynek Delroisse, Jérôme Bayaert, Wendy-Shirley Parey, Elise Oliveri, Paola Marlétaz, Ferdinand Marek, Martin Oakley, Todd H Echinodermata Animals Hydrolases Luciferases Genomics Evolution, Molecular Genome Kinetics Biocatalysis Phylogeny Functional Characterization of Luciferase in a Brittle Star Indicates Parallel Evolution Influenced by Genomic Availability of Haloalkane Dehalogenase. Lau, Emily S Majerova, Marika Hensley, Nicholai M Mukherjee, Arnab Vasina, Michal Pluskal, Daniel Damborsky, Jiri Prokop, Zbynek Delroisse, Jérôme Bayaert, Wendy-Shirley Parey, Elise Oliveri, Paola Marlétaz, Ferdinand Marek, Martin Oakley, Todd H Echinodermata Animals Hydrolases Luciferases Genomics Evolution, Molecular Genome Kinetics Biocatalysis Phylogeny Determining why convergent traits use distinct versus shared genetic components is crucial for understanding how evolutionary processes generate and sustain biodiversity. However, the factors dictating the genetic underpinnings of convergent traits remain incompletely understood. Here, we use heterologous protein expression, biochemical assays, and phylogenetic analyses to confirm the origin of a luciferase gene from haloalkane dehalogenases in the brittle star Amphiura filiformis. Through database searches and gene tree analyses, we also show a complex pattern of the presence and absence of haloalkane dehalogenases across organismal genomes. These results first confirm parallel evolution across a vast phylogenetic distance, because octocorals like Renilla also use luciferase derived from haloalkane dehalogenases. This parallel evolution is surprising, even though previously hypothesized, because many organisms that also use coelenterazine as the bioluminescence substrate evolved completely distinct luciferases. The inability to detect haloalkane dehalogenases in the genomes of several bioluminescent groups suggests that the distribution of this gene family influences its recruitment as a luciferase. Together, our findings highlight how biochemical function and genomic availability help determine whether distinct or shared genetic components are used during the convergent evolution of traits like bioluminescence. |
| title | Functional Characterization of Luciferase in a Brittle Star Indicates Parallel Evolution Influenced by Genomic Availability of Haloalkane Dehalogenase. |
| topic | Echinodermata Animals Hydrolases Luciferases Genomics Evolution, Molecular Genome Kinetics Biocatalysis Phylogeny |
| url | https://pubmed.ncbi.nlm.nih.gov/40181585/ |