Deciphering the Cross-Reactivity of Tropomyosin across Three Molluscan Species: Insights into the Role of Conserved T-Cell and B-Cell Epitopes.
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Journal of agricultural and food chemistry
2025
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| _version_ | 1868266221016711168 |
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| author | Han, Xin-Yu Huan, Fei Yang, Sheng-Yan He, Xin-Rong Lai, Dong Liu, Qing-Mei Tsui, Stephen Kwok-Wing Xiao, An-Feng Rao, Shi-Tao Liu, Guang-Ming |
| author_facet | Han, Xin-Yu Huan, Fei Yang, Sheng-Yan He, Xin-Rong Lai, Dong Liu, Qing-Mei Tsui, Stephen Kwok-Wing Xiao, An-Feng Rao, Shi-Tao Liu, Guang-Ming Han, Xin-Yu Huan, Fei Yang, Sheng-Yan He, Xin-Rong Lai, Dong Liu, Qing-Mei Tsui, Stephen Kwok-Wing Xiao, An-Feng Rao, Shi-Tao Liu, Guang-Ming |
| collection | PubMed - marine biology |
| contents | Deciphering the Cross-Reactivity of Tropomyosin across Three Molluscan Species: Insights into the Role of Conserved T-Cell and B-Cell Epitopes. Han, Xin-Yu Huan, Fei Yang, Sheng-Yan He, Xin-Rong Lai, Dong Liu, Qing-Mei Tsui, Stephen Kwok-Wing Xiao, An-Feng Rao, Shi-Tao Liu, Guang-Ming Animals Tropomyosin Cross Reactions Mice Epitopes, B-Lymphocyte Epitopes, T-Lymphocyte Mollusca Mice, Inbred BALB C Immunoglobulin E Allergens Female Humans Amino Acid Sequence tropomyosin (HTM), tropomyosin (ATM), and tropomyosin (MTM) were reported as significant seafood allergens capable of eliciting severe allergic reactions. However, studies of cross-reactivity and epitope mapping among them are notably limited. This study discovered their cross-reactivity property through inhibition of IgG/IgE binding capacity and promotion of specific CD4T-cell proliferation. For T-cell epitopes, 3, 4, and 3 epitopes were identified from the splenocytes of mice immunized with HTM, ATM, and MTM, respectively. In terms of B-cell epitopes, a combination of bioinformatics techniques and serological assays identified 4, 3, and 4 epitopes for HTM, ATM, and MTM, respectively. Sequence logo analysis revealed 2 conserved T-cell epitopes and 5 conserved B-cell epitopes, which may be critical for the observed cross-reactivity property. In conclusion, these findings provide crucial molecular evidence that could enhance the prevention of allergic reactions to aquatic mollusks. |
| format | Artículo científico |
| id | pubmed_40183940 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | Deciphering the Cross-Reactivity of Tropomyosin across Three Molluscan Species: Insights into the Role of Conserved T-Cell and B-Cell Epitopes. Han, Xin-Yu Huan, Fei Yang, Sheng-Yan He, Xin-Rong Lai, Dong Liu, Qing-Mei Tsui, Stephen Kwok-Wing Xiao, An-Feng Rao, Shi-Tao Liu, Guang-Ming Animals Tropomyosin Cross Reactions Mice Epitopes, B-Lymphocyte Epitopes, T-Lymphocyte Mollusca Mice, Inbred BALB C Immunoglobulin E Allergens Female Humans Amino Acid Sequence Deciphering the Cross-Reactivity of Tropomyosin across Three Molluscan Species: Insights into the Role of Conserved T-Cell and B-Cell Epitopes. Han, Xin-Yu Huan, Fei Yang, Sheng-Yan He, Xin-Rong Lai, Dong Liu, Qing-Mei Tsui, Stephen Kwok-Wing Xiao, An-Feng Rao, Shi-Tao Liu, Guang-Ming Animals Tropomyosin Cross Reactions Mice Epitopes, B-Lymphocyte Epitopes, T-Lymphocyte Mollusca Mice, Inbred BALB C Immunoglobulin E Allergens Female Humans Amino Acid Sequence tropomyosin (HTM), tropomyosin (ATM), and tropomyosin (MTM) were reported as significant seafood allergens capable of eliciting severe allergic reactions. However, studies of cross-reactivity and epitope mapping among them are notably limited. This study discovered their cross-reactivity property through inhibition of IgG/IgE binding capacity and promotion of specific CD4T-cell proliferation. For T-cell epitopes, 3, 4, and 3 epitopes were identified from the splenocytes of mice immunized with HTM, ATM, and MTM, respectively. In terms of B-cell epitopes, a combination of bioinformatics techniques and serological assays identified 4, 3, and 4 epitopes for HTM, ATM, and MTM, respectively. Sequence logo analysis revealed 2 conserved T-cell epitopes and 5 conserved B-cell epitopes, which may be critical for the observed cross-reactivity property. In conclusion, these findings provide crucial molecular evidence that could enhance the prevention of allergic reactions to aquatic mollusks. |
| title | Deciphering the Cross-Reactivity of Tropomyosin across Three Molluscan Species: Insights into the Role of Conserved T-Cell and B-Cell Epitopes. |
| topic | Animals Tropomyosin Cross Reactions Mice Epitopes, B-Lymphocyte Epitopes, T-Lymphocyte Mollusca Mice, Inbred BALB C Immunoglobulin E Allergens Female Humans Amino Acid Sequence |
| url | https://pubmed.ncbi.nlm.nih.gov/40183940/ |