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| Format: | Artículo científico |
| Sprache: | en |
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Plant science : an international journal of experimental plant biology
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40194683/ |
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| author | Shao, Zhizhuo Guo, Menglin Wang, Hong Gu, Wenhui Xie, Xiujun Wang, Guangce |
| author_facet | Shao, Zhizhuo Guo, Menglin Wang, Hong Gu, Wenhui Xie, Xiujun Wang, Guangce Shao, Zhizhuo Guo, Menglin Wang, Hong Gu, Wenhui Xie, Xiujun Wang, Guangce |
| collection | PubMed - marine biology |
| contents | Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis. Shao, Zhizhuo Guo, Menglin Wang, Hong Gu, Wenhui Xie, Xiujun Wang, Guangce Carbonic Anhydrases Carbon Reactive Oxygen Species Photosynthesis Rhodophyta NADPH Oxidases Edible Seaweeds Porphyra Pyropia yezoensis is an important intertidal economic macroalgae, which is periodically affected by various stresses, such as the limitation of inorganic carbon (C) deficiency. Under such environment, the redox homeostasis within the cells of P. yezoensis is seriously affected, and the reactive oxygen species (ROS) signal transduction system would be activated to regulate the photosynthetic activity. Therefore, how P. yezoensis manage ROS to maintain effective photosynthetic carbon fixation has aroused great interest. Here, we characterize transformants overexpressing respiratory burst oxidase homolog (Rboh), an important gene that can actively produce ROS, at the levels of cellular physiology, biochemistry, and transcriptomics. Our data indicated the expression of Rboh significantly increased, accompanied by a significant upregulated expression of alpha-type carbonic anhydrase 3 (αCA3) and increased extracellular carbonic anhydrase activity in the Rboh overexpressing strains. Interestingly, compared with the wild type, the photosynthetic activity of transgenic strains was significantly higher under the low C and high light condition, implying that the ROS signal triggered by overexpression of Rboh was involved in regulating the C absorption and utilization in P. yezoensis when the C source was limited. In summary, this study provided evidence supporting the correlation between the ROS production and the Ci utilization under stress environments in P. yezoensis. |
| format | Artículo científico |
| id | pubmed_40194683 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Plant science : an international journal of experimental plant biology |
| record_format | pubmed |
| spellingShingle | Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis. Shao, Zhizhuo Guo, Menglin Wang, Hong Gu, Wenhui Xie, Xiujun Wang, Guangce Carbonic Anhydrases Carbon Reactive Oxygen Species Photosynthesis Rhodophyta NADPH Oxidases Edible Seaweeds Porphyra Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis. Shao, Zhizhuo Guo, Menglin Wang, Hong Gu, Wenhui Xie, Xiujun Wang, Guangce Carbonic Anhydrases Carbon Reactive Oxygen Species Photosynthesis Rhodophyta NADPH Oxidases Edible Seaweeds Porphyra Pyropia yezoensis is an important intertidal economic macroalgae, which is periodically affected by various stresses, such as the limitation of inorganic carbon (C) deficiency. Under such environment, the redox homeostasis within the cells of P. yezoensis is seriously affected, and the reactive oxygen species (ROS) signal transduction system would be activated to regulate the photosynthetic activity. Therefore, how P. yezoensis manage ROS to maintain effective photosynthetic carbon fixation has aroused great interest. Here, we characterize transformants overexpressing respiratory burst oxidase homolog (Rboh), an important gene that can actively produce ROS, at the levels of cellular physiology, biochemistry, and transcriptomics. Our data indicated the expression of Rboh significantly increased, accompanied by a significant upregulated expression of alpha-type carbonic anhydrase 3 (αCA3) and increased extracellular carbonic anhydrase activity in the Rboh overexpressing strains. Interestingly, compared with the wild type, the photosynthetic activity of transgenic strains was significantly higher under the low C and high light condition, implying that the ROS signal triggered by overexpression of Rboh was involved in regulating the C absorption and utilization in P. yezoensis when the C source was limited. In summary, this study provided evidence supporting the correlation between the ROS production and the Ci utilization under stress environments in P. yezoensis. |
| title | Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis. |
| topic | Carbonic Anhydrases Carbon Reactive Oxygen Species Photosynthesis Rhodophyta NADPH Oxidases Edible Seaweeds Porphyra |
| url | https://pubmed.ncbi.nlm.nih.gov/40194683/ |