Synthesis of capsular oligosaccharides and identification of a potential -antigen against campylobacteriosis.
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| Format: | Artículo científico |
| Sprache: | en |
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Science advances
2025
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| _version_ | 1868266220985253888 |
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| author | Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming |
| author_facet | Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming |
| collection | PubMed - marine biology |
| contents | Synthesis of capsular oligosaccharides and identification of a potential -antigen against campylobacteriosis. Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming Campylobacter jejuni O Antigens Oligosaccharides Campylobacter Infections Animals Bacterial Capsules Bacterial Vaccines The vaccines against campylobacteriosis are urgently needed because of the rising multidrug resistance of pathogenic . The capsular polysaccharides of these bacteria, containing unique 6-deoxy-β-d--heptopyranosyl or l--β-d--heptopyranosyl residues, have emerged as attractive antigens. Expeditious assembly of the oligosaccharides derived from these glycans is challenging because β-d-idopyranosidic linkages are formidable to directly construct. Furthermore, whether the synthetic oligosaccharides could induce sufficient immunogenicity as the potential antigens remains unexplored. Here, we report a protocol for directly forming β-d-idopyranosidic bonds using α-d-6-deoxy--heptopyranosyl, α-d-idopyranosyl, and d-/l--α-d--heptopyranosyl -hexynylbenzoates as glycosylating agents under gold(I) catalysis. To demonstrate the versatility of these methods, concise synthesis of conjugatable capsular di-/tetra-/hexa-/octasaccharides, having a backbone of [→3)-6-deoxy-β-d--heptopyranosyl-(1→4)-2-acetamido-2-deoxy-β-d-glucopyranosyl-(1→], has been achieved. The immunogenicity assessment of the glycoconjugates, prepared by conjugating the synthesized oligosaccharides to cross-reactive material 197, reveals the disaccharide as a potential -antigen for developing vaccines against campylobacteriosis. This work should facilitate development of synthetic vaccines against infections. |
| format | Artículo científico |
| id | pubmed_40203105 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Science advances |
| record_format | pubmed |
| spellingShingle | Synthesis of capsular oligosaccharides and identification of a potential -antigen against campylobacteriosis. Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming Campylobacter jejuni O Antigens Oligosaccharides Campylobacter Infections Animals Bacterial Capsules Bacterial Vaccines Synthesis of capsular oligosaccharides and identification of a potential -antigen against campylobacteriosis. Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming Campylobacter jejuni O Antigens Oligosaccharides Campylobacter Infections Animals Bacterial Capsules Bacterial Vaccines The vaccines against campylobacteriosis are urgently needed because of the rising multidrug resistance of pathogenic . The capsular polysaccharides of these bacteria, containing unique 6-deoxy-β-d--heptopyranosyl or l--β-d--heptopyranosyl residues, have emerged as attractive antigens. Expeditious assembly of the oligosaccharides derived from these glycans is challenging because β-d-idopyranosidic linkages are formidable to directly construct. Furthermore, whether the synthetic oligosaccharides could induce sufficient immunogenicity as the potential antigens remains unexplored. Here, we report a protocol for directly forming β-d-idopyranosidic bonds using α-d-6-deoxy--heptopyranosyl, α-d-idopyranosyl, and d-/l--α-d--heptopyranosyl -hexynylbenzoates as glycosylating agents under gold(I) catalysis. To demonstrate the versatility of these methods, concise synthesis of conjugatable capsular di-/tetra-/hexa-/octasaccharides, having a backbone of [→3)-6-deoxy-β-d--heptopyranosyl-(1→4)-2-acetamido-2-deoxy-β-d-glucopyranosyl-(1→], has been achieved. The immunogenicity assessment of the glycoconjugates, prepared by conjugating the synthesized oligosaccharides to cross-reactive material 197, reveals the disaccharide as a potential -antigen for developing vaccines against campylobacteriosis. This work should facilitate development of synthetic vaccines against infections. |
| title | Synthesis of capsular oligosaccharides and identification of a potential -antigen against campylobacteriosis. |
| topic | Campylobacter jejuni O Antigens Oligosaccharides Campylobacter Infections Animals Bacterial Capsules Bacterial Vaccines |
| url | https://pubmed.ncbi.nlm.nih.gov/40203105/ |