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| Autori principali: | , , , , , , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Science advances
2025
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40203105/ |
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Sommario:
- Synthesis of capsular oligosaccharides and identification of a potential -antigen against campylobacteriosis. Wang, Jianjun Yang, Xuemei Huang, Zirong Su, Yong Zhang, Xinxin Song, Ni Wang, Peng Yang, Chen Cao, Hongzhi Liu, Xue-Wei Li, Xuechen Chen, Sheng Li, Ming Campylobacter jejuni O Antigens Oligosaccharides Campylobacter Infections Animals Bacterial Capsules Bacterial Vaccines The vaccines against campylobacteriosis are urgently needed because of the rising multidrug resistance of pathogenic . The capsular polysaccharides of these bacteria, containing unique 6-deoxy-β-d--heptopyranosyl or l--β-d--heptopyranosyl residues, have emerged as attractive antigens. Expeditious assembly of the oligosaccharides derived from these glycans is challenging because β-d-idopyranosidic linkages are formidable to directly construct. Furthermore, whether the synthetic oligosaccharides could induce sufficient immunogenicity as the potential antigens remains unexplored. Here, we report a protocol for directly forming β-d-idopyranosidic bonds using α-d-6-deoxy--heptopyranosyl, α-d-idopyranosyl, and d-/l--α-d--heptopyranosyl -hexynylbenzoates as glycosylating agents under gold(I) catalysis. To demonstrate the versatility of these methods, concise synthesis of conjugatable capsular di-/tetra-/hexa-/octasaccharides, having a backbone of [→3)-6-deoxy-β-d--heptopyranosyl-(1→4)-2-acetamido-2-deoxy-β-d-glucopyranosyl-(1→], has been achieved. The immunogenicity assessment of the glycoconjugates, prepared by conjugating the synthesized oligosaccharides to cross-reactive material 197, reveals the disaccharide as a potential -antigen for developing vaccines against campylobacteriosis. This work should facilitate development of synthetic vaccines against infections.