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| Main Authors: | , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
STAR protocols
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40220302/ |
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| _version_ | 1868266216494202880 |
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| author | Wei, Chaoguang Jia, Ruonan Canário, Adelino V M Chen, Liangbiao Xu, Qianghua Hu, Peng |
| author_facet | Wei, Chaoguang Jia, Ruonan Canário, Adelino V M Chen, Liangbiao Xu, Qianghua Hu, Peng Wei, Chaoguang Jia, Ruonan Canário, Adelino V M Chen, Liangbiao Xu, Qianghua Hu, Peng |
| collection | PubMed - marine biology |
| contents | Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays. Wei, Chaoguang Jia, Ruonan Canário, Adelino V M Chen, Liangbiao Xu, Qianghua Hu, Peng Animals Cryopreservation Single-Cell Analysis Cell Nucleus Myocardium Genomics Fishes Heart Unbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen heart tissue, library construction, and sequencing. This protocol can provide a valuable reference for isolating nuclei from other cryopreserved marine fish tissues. |
| format | Artículo científico |
| id | pubmed_40220302 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | STAR protocols |
| record_format | pubmed |
| spellingShingle | Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays. Wei, Chaoguang Jia, Ruonan Canário, Adelino V M Chen, Liangbiao Xu, Qianghua Hu, Peng Animals Cryopreservation Single-Cell Analysis Cell Nucleus Myocardium Genomics Fishes Heart Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays. Wei, Chaoguang Jia, Ruonan Canário, Adelino V M Chen, Liangbiao Xu, Qianghua Hu, Peng Animals Cryopreservation Single-Cell Analysis Cell Nucleus Myocardium Genomics Fishes Heart Unbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen heart tissue, library construction, and sequencing. This protocol can provide a valuable reference for isolating nuclei from other cryopreserved marine fish tissues. |
| title | Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays. |
| topic | Animals Cryopreservation Single-Cell Analysis Cell Nucleus Myocardium Genomics Fishes Heart |
| url | https://pubmed.ncbi.nlm.nih.gov/40220302/ |