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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of hazardous materials
2025
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/40220380/ |
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- In Vitro metabolism of six representative organic UV stabilizers (OUVs) in marine fish liver microsomes: Kinetics, mechanisms and bioaccumulation evaluation. Wang, Yuchen Huang, Qianyi Zhang, Siqi Liu, Shan Li, Hengxiang Wang, Xiaodong Wang, Yan Hou, Rui Xu, Xiangrong Animals Microsomes, Liver Water Pollutants, Chemical Kinetics Bioaccumulation Fishes Molecular Docking Simulation Perciformes Cytochrome P-450 CYP3A The ubiquitous occurrence of organic UV stabilizers (OUVs) poses a potential threat to marine fish, but the metabolism of OUVs in the body remains a missing piece of the puzzle in their ecotoxicology. This study investigated the in vitro metabolism of six OUVs in the liver microsomes of an economically valuable marine fish (Lateolabrax japonicus). The depletion of the six OUVs follows first-order kinetics in the microsomes, with in vitro depletion rate constants (k) ranging from 0.23 to 0.40/h. The intrinsic clearance (CL) of 1H-benzotriazole (BT) was estimated to be 0.0026 μL/(min·mg·protein). The enzyme inhibition experiments and molecular docking results highlight the importance of cytochrome P450 enzyme (CYP) 3A4 in OUVs metabolism. Among the 4 metabolizable compounds, some metabolites from hydroxylation, methylation, acetylation and conjugation were tentatively identified. In addition, the in vitro transformation rates can more accurately predict the whole-body bioconcentration potential via an in vitro to in vivo extrapolation (IVIVE) model.