The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.

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Autore principale: Serôdio, João
Natura: Artículo científico
Lingua:en
Pubblicazione: Photosynthesis research 2025
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author Serôdio, João
author_facet Serôdio, João
Serôdio, João
collection PubMed - marine biology
contents The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes. Serôdio, João Chlorophyll Fluorescence Plant Leaves Photosynthesis In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.
format Artículo científico
id pubmed_40232522
institution PubMed
language en
publishDate 2025
publisher Photosynthesis research
record_format pubmed
spellingShingle The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.
Serôdio, João
Chlorophyll
Fluorescence
Plant Leaves
Photosynthesis
The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes. Serôdio, João Chlorophyll Fluorescence Plant Leaves Photosynthesis In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.
title The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.
topic Chlorophyll
Fluorescence
Plant Leaves
Photosynthesis
url https://pubmed.ncbi.nlm.nih.gov/40232522/