An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging.
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Scientific reports
2025
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| _version_ | 1868266216477425665 |
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| author | Gao, Chao Wu, Yan Fitzgerald, Connor Wang, Hui Ugo, Tim Uyeda, Tetsuo Zhou, Wenhui Su, Yichi Kirkland, Thomas A Lin, Michael Z |
| author_facet | Gao, Chao Wu, Yan Fitzgerald, Connor Wang, Hui Ugo, Tim Uyeda, Tetsuo Zhou, Wenhui Su, Yichi Kirkland, Thomas A Lin, Michael Z Gao, Chao Wu, Yan Fitzgerald, Connor Wang, Hui Ugo, Tim Uyeda, Tetsuo Zhou, Wenhui Su, Yichi Kirkland, Thomas A Lin, Michael Z |
| collection | PubMed - marine biology |
| contents | An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging. Gao, Chao Wu, Yan Fitzgerald, Connor Wang, Hui Ugo, Tim Uyeda, Tetsuo Zhou, Wenhui Su, Yichi Kirkland, Thomas A Lin, Michael Z Animals Luminescent Measurements Luciferases Mice Nanoparticles Luminescent Agents Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo. With recent improvements in BLI brightness from the engineering of bioluminescent enzymes (luciferases) and substrates (luciferins), optimizing luciferin formulations to maximize delivery and minimize toxicity becomes important, especially for marine coelenterazine-type luciferins with limited solubility. Here, we complete the characterization of a previously reported NanoLuc substrate, designated cephalofurimazine-9 (CFz9), and optimize its formulation with water-soluble excipients. We report a pH-controlled formulation of CFz9 enabling high-dose delivery to achieve peak brightness comparable to other furimazine analogs both inside and outside the brain while reducing toxicity. Thus, an optimized CFz9 formulation improves the performance and tolerability of whole-animal BLI with NanoLuc-based reporters. |
| format | Artículo científico |
| id | pubmed_40234651 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Scientific reports |
| record_format | pubmed |
| spellingShingle | An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging. Gao, Chao Wu, Yan Fitzgerald, Connor Wang, Hui Ugo, Tim Uyeda, Tetsuo Zhou, Wenhui Su, Yichi Kirkland, Thomas A Lin, Michael Z Animals Luminescent Measurements Luciferases Mice Nanoparticles Luminescent Agents An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging. Gao, Chao Wu, Yan Fitzgerald, Connor Wang, Hui Ugo, Tim Uyeda, Tetsuo Zhou, Wenhui Su, Yichi Kirkland, Thomas A Lin, Michael Z Animals Luminescent Measurements Luciferases Mice Nanoparticles Luminescent Agents Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo. With recent improvements in BLI brightness from the engineering of bioluminescent enzymes (luciferases) and substrates (luciferins), optimizing luciferin formulations to maximize delivery and minimize toxicity becomes important, especially for marine coelenterazine-type luciferins with limited solubility. Here, we complete the characterization of a previously reported NanoLuc substrate, designated cephalofurimazine-9 (CFz9), and optimize its formulation with water-soluble excipients. We report a pH-controlled formulation of CFz9 enabling high-dose delivery to achieve peak brightness comparable to other furimazine analogs both inside and outside the brain while reducing toxicity. Thus, an optimized CFz9 formulation improves the performance and tolerability of whole-animal BLI with NanoLuc-based reporters. |
| title | An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging. |
| topic | Animals Luminescent Measurements Luciferases Mice Nanoparticles Luminescent Agents |
| url | https://pubmed.ncbi.nlm.nih.gov/40234651/ |