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| Auteurs principaux: | , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Fish & shellfish immunology
2025
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40239933/ |
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| _version_ | 1868266216469037056 |
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| author | Tian, Ruotong Geng, Shang Lv, Xing Li, Wenhui Xu, Tianjun Sun, Yuena |
| author_facet | Tian, Ruotong Geng, Shang Lv, Xing Li, Wenhui Xu, Tianjun Sun, Yuena Tian, Ruotong Geng, Shang Lv, Xing Li, Wenhui Xu, Tianjun Sun, Yuena |
| collection | PubMed - marine biology |
| contents | Evolutionary insights and poly(I:C)-induced changes in expression and mA modifications of il17ra and il17rc in Miichthysmiiuy. Tian, Ruotong Geng, Shang Lv, Xing Li, Wenhui Xu, Tianjun Sun, Yuena Animals Poly I-C Receptors, Interleukin-17 Fish Proteins Perciformes Gene Expression Regulation Immunity, Innate Adenosine Phylogeny Interleukin-17 receptor A (IL17RA) and IL17RC form a receptor complex critical for initiating IL-17A-mediated immune responses, a hallmark of T helper 17 (Th17) cells. In this study, il17ra and il17rc were identified in miiuy croaker (Miichthys miiuy) and bioinformatics analysis revealed their evolutionary and structural conservation, underscoring their roles in immunity. However, there has been little research on the IL-17 receptor family from the perspective of N6-methyladenosine (mA) modifications. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we identified strong mA modifications on the last exons of il17ra and il17rc, validated by MeRIP-PCR. Poly(I:C) stimulation significantly upregulated il17ra and il17rc expression, while reducing their mA modification levels, implicating these changes in antiviral immunity. Interestingly, cycloleucine (CL), a well-known methylation inhibitor, did not alter the expression of il17ra and il17rc but promoted the nuclear-to-cytoplasmic transport of il17ra mRNA, potentially influencing its translation process. These findings provide valuable insights into the regulatory roles of mA modifications in il17ra and il17rc function and highlight their importance in the antiviral immune response. |
| format | Artículo científico |
| id | pubmed_40239933 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Evolutionary insights and poly(I:C)-induced changes in expression and mA modifications of il17ra and il17rc in Miichthysmiiuy. Tian, Ruotong Geng, Shang Lv, Xing Li, Wenhui Xu, Tianjun Sun, Yuena Animals Poly I-C Receptors, Interleukin-17 Fish Proteins Perciformes Gene Expression Regulation Immunity, Innate Adenosine Phylogeny Evolutionary insights and poly(I:C)-induced changes in expression and mA modifications of il17ra and il17rc in Miichthysmiiuy. Tian, Ruotong Geng, Shang Lv, Xing Li, Wenhui Xu, Tianjun Sun, Yuena Animals Poly I-C Receptors, Interleukin-17 Fish Proteins Perciformes Gene Expression Regulation Immunity, Innate Adenosine Phylogeny Interleukin-17 receptor A (IL17RA) and IL17RC form a receptor complex critical for initiating IL-17A-mediated immune responses, a hallmark of T helper 17 (Th17) cells. In this study, il17ra and il17rc were identified in miiuy croaker (Miichthys miiuy) and bioinformatics analysis revealed their evolutionary and structural conservation, underscoring their roles in immunity. However, there has been little research on the IL-17 receptor family from the perspective of N6-methyladenosine (mA) modifications. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we identified strong mA modifications on the last exons of il17ra and il17rc, validated by MeRIP-PCR. Poly(I:C) stimulation significantly upregulated il17ra and il17rc expression, while reducing their mA modification levels, implicating these changes in antiviral immunity. Interestingly, cycloleucine (CL), a well-known methylation inhibitor, did not alter the expression of il17ra and il17rc but promoted the nuclear-to-cytoplasmic transport of il17ra mRNA, potentially influencing its translation process. These findings provide valuable insights into the regulatory roles of mA modifications in il17ra and il17rc function and highlight their importance in the antiviral immune response. |
| title | Evolutionary insights and poly(I:C)-induced changes in expression and mA modifications of il17ra and il17rc in Miichthysmiiuy. |
| topic | Animals Poly I-C Receptors, Interleukin-17 Fish Proteins Perciformes Gene Expression Regulation Immunity, Innate Adenosine Phylogeny |
| url | https://pubmed.ncbi.nlm.nih.gov/40239933/ |