Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone ().
Fuente:
PubMed
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of molecular sciences
2025
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266214137004033 |
|---|---|
| author | Huang, Jianfang Zhou, Mingcan She, Zhenghan Chen, Jianming Ke, Caihuan |
| author_facet | Huang, Jianfang Zhou, Mingcan She, Zhenghan Chen, Jianming Ke, Caihuan Huang, Jianfang Zhou, Mingcan She, Zhenghan Chen, Jianming Ke, Caihuan |
| collection | PubMed - marine biology |
| contents | Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (). Huang, Jianfang Zhou, Mingcan She, Zhenghan Chen, Jianming Ke, Caihuan Animals MicroRNAs Female Male Gastropoda Reproduction Ovary RNA, Messenger Testis Gene Expression Profiling Transcriptome Gonads Computational Biology Reproduction and breeding are crucial to maintaining abalone aquaculture. Understanding the molecular underpinnings of sexual maturation is essential for advancing knowledge in reproductive biology. However, the molecular mechanisms of gonadal development in abalones remain poorly understood, particularly in microRNA (miRNA)-mediated regulation. Thus, this study conducted a comprehensive transcriptomic analysis of abalone () to identify genes and miRNAs associated with ovarian and testicular discovery. This study identified 685 differentially expressed (DE) genes between the ovary (DD_ovary) and testis (DD_testis) groups, comprising 479 upregulated and 206 downregulated genes in the DD_ovary. Moreover, 137 miRNAs, including 83 novel and 54 known miRNAs, were detected, with 30 upregulated and 27 downregulated in the DD_ovary compared to the DD_testis. Bioinformatics analysis revealed that these miRNAs regulate key processes such as carbohydrate metabolic processes, kinase and hydrolase activity, and starch and sucrose metabolism, all potentially associated with reproductive traits. Further, key mRNA candidates, including () and () 1, and miRNAs such as novel_90 and novel_120, were identified as components of a functional miRNA-mRNA network associated with sexual maturity and sex determination. These key genes were verified using qRT-PCR and fluorescence in situ hybridization (FISH). These transcriptomic and miRNA datasets provide valuable resources for understanding abalone reproductive biology and may support molecular breeding strategies. |
| format | Artículo científico |
| id | pubmed_40244070 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (). Huang, Jianfang Zhou, Mingcan She, Zhenghan Chen, Jianming Ke, Caihuan Animals MicroRNAs Female Male Gastropoda Reproduction Ovary RNA, Messenger Testis Gene Expression Profiling Transcriptome Gonads Computational Biology Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (). Huang, Jianfang Zhou, Mingcan She, Zhenghan Chen, Jianming Ke, Caihuan Animals MicroRNAs Female Male Gastropoda Reproduction Ovary RNA, Messenger Testis Gene Expression Profiling Transcriptome Gonads Computational Biology Reproduction and breeding are crucial to maintaining abalone aquaculture. Understanding the molecular underpinnings of sexual maturation is essential for advancing knowledge in reproductive biology. However, the molecular mechanisms of gonadal development in abalones remain poorly understood, particularly in microRNA (miRNA)-mediated regulation. Thus, this study conducted a comprehensive transcriptomic analysis of abalone () to identify genes and miRNAs associated with ovarian and testicular discovery. This study identified 685 differentially expressed (DE) genes between the ovary (DD_ovary) and testis (DD_testis) groups, comprising 479 upregulated and 206 downregulated genes in the DD_ovary. Moreover, 137 miRNAs, including 83 novel and 54 known miRNAs, were detected, with 30 upregulated and 27 downregulated in the DD_ovary compared to the DD_testis. Bioinformatics analysis revealed that these miRNAs regulate key processes such as carbohydrate metabolic processes, kinase and hydrolase activity, and starch and sucrose metabolism, all potentially associated with reproductive traits. Further, key mRNA candidates, including () and () 1, and miRNAs such as novel_90 and novel_120, were identified as components of a functional miRNA-mRNA network associated with sexual maturity and sex determination. These key genes were verified using qRT-PCR and fluorescence in situ hybridization (FISH). These transcriptomic and miRNA datasets provide valuable resources for understanding abalone reproductive biology and may support molecular breeding strategies. |
| title | Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (). |
| topic | Animals MicroRNAs Female Male Gastropoda Reproduction Ovary RNA, Messenger Testis Gene Expression Profiling Transcriptome Gonads Computational Biology |
| url | https://pubmed.ncbi.nlm.nih.gov/40244070/ |