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| Auteurs principaux: | , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
International journal of molecular sciences
2025
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40244297/ |
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| _version_ | 1868266214135955457 |
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| author | Li, Weichen Liang, Xiaoqian Xiang, Keyu Li, Hongyan Zhang, Yu |
| author_facet | Li, Weichen Liang, Xiaoqian Xiang, Keyu Li, Hongyan Zhang, Yu Li, Weichen Liang, Xiaoqian Xiang, Keyu Li, Hongyan Zhang, Yu |
| collection | PubMed - marine biology |
| contents | The Ancestral KEAP1-NRF Pathway in Amphioxus : Implications for the Evolution of Antioxidant Defense System. Li, Weichen Liang, Xiaoqian Xiang, Keyu Li, Hongyan Zhang, Yu Animals Kelch-Like ECH-Associated Protein 1 Lancelets NF-E2-Related Factor 2 Antioxidants Signal Transduction Phylogeny Evolution, Molecular Oxidative Stress Humans The Kelch-like ECH-associated protein 1 (KEAP1)/Nuclear factor E2-related factor 2 (NRF2) pathway is a key mechanism that responds to oxidative stress and xenobiotic stimuli in vertebrates. However, knowledge of its evolutionary origins remains limited. In this study, we identify the ancestral homologues of KEAP1 and NRF (BjKEAP1 and BjNRF) in cephalochordate amphioxus (). BjNRF uniquely combines the feature domains of vertebrates NRF1 and NRF2, marking it as an evolutionary intermediate. High expression levels of 1 and in the gill, hepatic cecum, and intestine highlight their roles in environmental defense at key interface tissues. Functional studies reveal that BjKEAP1 regulates the cytoplasmic localization of BjNRF. Typical NRF2 activator sulforaphane (SFN) induces its nuclear translocation and significantly elevates the transcriptional expression of BjNRF and phase II detoxification enzymes. Moreover, exposure to the environmental toxin Benzo[a]pyrene (BaP) activates this stress response system. These findings bridge critical gaps in our understanding of this pathway in basal chordates and offer new insights into the evolutionary trajectory of the KEAP1-NRF system. Furthermore, this study highlights crucial implications for the conservation of amphioxus in deteriorating marine environments. |
| format | Artículo científico |
| id | pubmed_40244297 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | The Ancestral KEAP1-NRF Pathway in Amphioxus : Implications for the Evolution of Antioxidant Defense System. Li, Weichen Liang, Xiaoqian Xiang, Keyu Li, Hongyan Zhang, Yu Animals Kelch-Like ECH-Associated Protein 1 Lancelets NF-E2-Related Factor 2 Antioxidants Signal Transduction Phylogeny Evolution, Molecular Oxidative Stress Humans The Ancestral KEAP1-NRF Pathway in Amphioxus : Implications for the Evolution of Antioxidant Defense System. Li, Weichen Liang, Xiaoqian Xiang, Keyu Li, Hongyan Zhang, Yu Animals Kelch-Like ECH-Associated Protein 1 Lancelets NF-E2-Related Factor 2 Antioxidants Signal Transduction Phylogeny Evolution, Molecular Oxidative Stress Humans The Kelch-like ECH-associated protein 1 (KEAP1)/Nuclear factor E2-related factor 2 (NRF2) pathway is a key mechanism that responds to oxidative stress and xenobiotic stimuli in vertebrates. However, knowledge of its evolutionary origins remains limited. In this study, we identify the ancestral homologues of KEAP1 and NRF (BjKEAP1 and BjNRF) in cephalochordate amphioxus (). BjNRF uniquely combines the feature domains of vertebrates NRF1 and NRF2, marking it as an evolutionary intermediate. High expression levels of 1 and in the gill, hepatic cecum, and intestine highlight their roles in environmental defense at key interface tissues. Functional studies reveal that BjKEAP1 regulates the cytoplasmic localization of BjNRF. Typical NRF2 activator sulforaphane (SFN) induces its nuclear translocation and significantly elevates the transcriptional expression of BjNRF and phase II detoxification enzymes. Moreover, exposure to the environmental toxin Benzo[a]pyrene (BaP) activates this stress response system. These findings bridge critical gaps in our understanding of this pathway in basal chordates and offer new insights into the evolutionary trajectory of the KEAP1-NRF system. Furthermore, this study highlights crucial implications for the conservation of amphioxus in deteriorating marine environments. |
| title | The Ancestral KEAP1-NRF Pathway in Amphioxus : Implications for the Evolution of Antioxidant Defense System. |
| topic | Animals Kelch-Like ECH-Associated Protein 1 Lancelets NF-E2-Related Factor 2 Antioxidants Signal Transduction Phylogeny Evolution, Molecular Oxidative Stress Humans |
| url | https://pubmed.ncbi.nlm.nih.gov/40244297/ |