Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish.
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| Autori principali: | , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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The Science of the total environment
2025
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| author | Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K |
| author_facet | Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K |
| collection | PubMed - marine biology |
| contents | Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish. Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K Animals Tetrodotoxin Tetraodontiformes Food Contamination Aptamers, Nucleotide Seafood Environmental Monitoring Limit of Detection Antibodies Gold Tetrodotoxin (TTX) is a highly potent marine toxin which can cause severe poisoning following consumption of contaminated fish and seafood. Thus, a sensitive, reliable and simple test is required for rapid screening of samples and prevention of intoxication. Herein, we translated a previously reported microtiter plate hybrid aptamer-antibody assay into a rapid lateral flow assay (LFA) test. The test relies on an aptamer immobilized on the membrane and an antibody conjugated with gold nanoparticles to provide a visual result when TTX is present in the sample. The optimized test is simple (one-step), rapid ( |
| format | Artículo científico |
| id | pubmed_40245514 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | The Science of the total environment |
| record_format | pubmed |
| spellingShingle | Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish. Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K Animals Tetrodotoxin Tetraodontiformes Food Contamination Aptamers, Nucleotide Seafood Environmental Monitoring Limit of Detection Antibodies Gold Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish. Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K Animals Tetrodotoxin Tetraodontiformes Food Contamination Aptamers, Nucleotide Seafood Environmental Monitoring Limit of Detection Antibodies Gold Tetrodotoxin (TTX) is a highly potent marine toxin which can cause severe poisoning following consumption of contaminated fish and seafood. Thus, a sensitive, reliable and simple test is required for rapid screening of samples and prevention of intoxication. Herein, we translated a previously reported microtiter plate hybrid aptamer-antibody assay into a rapid lateral flow assay (LFA) test. The test relies on an aptamer immobilized on the membrane and an antibody conjugated with gold nanoparticles to provide a visual result when TTX is present in the sample. The optimized test is simple (one-step), rapid ( |
| title | Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish. |
| topic | Animals Tetrodotoxin Tetraodontiformes Food Contamination Aptamers, Nucleotide Seafood Environmental Monitoring Limit of Detection Antibodies Gold |
| url | https://pubmed.ncbi.nlm.nih.gov/40245514/ |