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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
The Science of the total environment
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40245514/ |
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Table of Contents:
- Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish. Díaz-Avello, Ulises G Skouridou, Vasso Shkembi, Xhensila Reverté, Jaume Mandalakis, Manolis Peristeraki, Panagiota Campàs, Mònica O'Sullivan, Ciara K Animals Tetrodotoxin Tetraodontiformes Food Contamination Aptamers, Nucleotide Seafood Environmental Monitoring Limit of Detection Antibodies Gold Tetrodotoxin (TTX) is a highly potent marine toxin which can cause severe poisoning following consumption of contaminated fish and seafood. Thus, a sensitive, reliable and simple test is required for rapid screening of samples and prevention of intoxication. Herein, we translated a previously reported microtiter plate hybrid aptamer-antibody assay into a rapid lateral flow assay (LFA) test. The test relies on an aptamer immobilized on the membrane and an antibody conjugated with gold nanoparticles to provide a visual result when TTX is present in the sample. The optimized test is simple (one-step), rapid (