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| Auteurs principaux: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Nature communications
2025
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40253397/ |
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| author | Li, Lei Lu, Meina Guo, Lidong Zhang, Xuejiao Liu, Qun Zhang, Meiling Gao, Junying Xu, Mengyang Lu, Yijian Zhang, Fang Li, Yao Zhang, Ruihua Liu, Xiawei Pan, Shanshan Zhang, Xianghui Li, Zhen Chen, Yadong Su, Xiaoshan Zhang, Nannan Guo, Wenjie Yang, Tao Chen, Jing Qin, Yating Zhang, Zhe Cui, Wei Yu, Lindong Gu, Ying Yang, Huanming Xu, Xun Wang, Jianxun Burns, Caroline E Burns, C Geoffrey Han, Kai Zhao, Long Fan, Guangyi Su, Ying |
| author_facet | Li, Lei Lu, Meina Guo, Lidong Zhang, Xuejiao Liu, Qun Zhang, Meiling Gao, Junying Xu, Mengyang Lu, Yijian Zhang, Fang Li, Yao Zhang, Ruihua Liu, Xiawei Pan, Shanshan Zhang, Xianghui Li, Zhen Chen, Yadong Su, Xiaoshan Zhang, Nannan Guo, Wenjie Yang, Tao Chen, Jing Qin, Yating Zhang, Zhe Cui, Wei Yu, Lindong Gu, Ying Yang, Huanming Xu, Xun Wang, Jianxun Burns, Caroline E Burns, C Geoffrey Han, Kai Zhao, Long Fan, Guangyi Su, Ying Li, Lei Lu, Meina Guo, Lidong Zhang, Xuejiao Liu, Qun Zhang, Meiling Gao, Junying Xu, Mengyang Lu, Yijian Zhang, Fang Li, Yao Zhang, Ruihua Liu, Xiawei Pan, Shanshan Zhang, Xianghui Li, Zhen Chen, Yadong Su, Xiaoshan Zhang, Nannan Guo, Wenjie Yang, Tao Chen, Jing Qin, Yating Zhang, Zhe Cui, Wei Yu, Lindong Gu, Ying Yang, Huanming Xu, Xun Wang, Jianxun Burns, Caroline E Burns, C Geoffrey Han, Kai Zhao, Long Fan, Guangyi Su, Ying |
| collection | PubMed - marine biology |
| contents | An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart. Li, Lei Lu, Meina Guo, Lidong Zhang, Xuejiao Liu, Qun Zhang, Meiling Gao, Junying Xu, Mengyang Lu, Yijian Zhang, Fang Li, Yao Zhang, Ruihua Liu, Xiawei Pan, Shanshan Zhang, Xianghui Li, Zhen Chen, Yadong Su, Xiaoshan Zhang, Nannan Guo, Wenjie Yang, Tao Chen, Jing Qin, Yating Zhang, Zhe Cui, Wei Yu, Lindong Gu, Ying Yang, Huanming Xu, Xun Wang, Jianxun Burns, Caroline E Burns, C Geoffrey Han, Kai Zhao, Long Fan, Guangyi Su, Ying Animals Zebrafish Regeneration Heart Myocytes, Cardiac Transcriptome Zebrafish Proteins Single-Cell Analysis Myocardium Cell Differentiation Gene Expression Profiling Adult zebrafish robustly regenerate injured hearts through a complex orchestration of molecular and cellular activities. However, this remarkable process, which is largely non-existent in humans, remains incompletely understood. Here, we utilize integrated spatial transcriptomics (Stereo-seq) and single-cell RNA-sequencing (scRNA-seq) to generate a spatially-resolved molecular and cellular atlas of regenerating zebrafish heart across eight stages. We characterize the cascade of cardiomyocyte cell states responsible for producing regenerated myocardium and explore a potential role for tpm4a in cardiomyocyte re-differentiation. Moreover, we uncover the activation of ifrd1 and atp6ap2 genes as a unique feature of regenerative hearts. Lastly, we reconstruct a 4D "virtual regenerating heart" comprising 569,896 cells/spots derived from 36 scRNA-seq libraries and 224 Stereo-seq slices. Our comprehensive atlas serves as a valuable resource to the cardiovascular and regeneration scientific communities and their ongoing efforts to understand the molecular and cellular mechanisms underlying vertebrate heart regeneration. |
| format | Artículo científico |
| id | pubmed_40253397 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart. Li, Lei Lu, Meina Guo, Lidong Zhang, Xuejiao Liu, Qun Zhang, Meiling Gao, Junying Xu, Mengyang Lu, Yijian Zhang, Fang Li, Yao Zhang, Ruihua Liu, Xiawei Pan, Shanshan Zhang, Xianghui Li, Zhen Chen, Yadong Su, Xiaoshan Zhang, Nannan Guo, Wenjie Yang, Tao Chen, Jing Qin, Yating Zhang, Zhe Cui, Wei Yu, Lindong Gu, Ying Yang, Huanming Xu, Xun Wang, Jianxun Burns, Caroline E Burns, C Geoffrey Han, Kai Zhao, Long Fan, Guangyi Su, Ying Animals Zebrafish Regeneration Heart Myocytes, Cardiac Transcriptome Zebrafish Proteins Single-Cell Analysis Myocardium Cell Differentiation Gene Expression Profiling An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart. Li, Lei Lu, Meina Guo, Lidong Zhang, Xuejiao Liu, Qun Zhang, Meiling Gao, Junying Xu, Mengyang Lu, Yijian Zhang, Fang Li, Yao Zhang, Ruihua Liu, Xiawei Pan, Shanshan Zhang, Xianghui Li, Zhen Chen, Yadong Su, Xiaoshan Zhang, Nannan Guo, Wenjie Yang, Tao Chen, Jing Qin, Yating Zhang, Zhe Cui, Wei Yu, Lindong Gu, Ying Yang, Huanming Xu, Xun Wang, Jianxun Burns, Caroline E Burns, C Geoffrey Han, Kai Zhao, Long Fan, Guangyi Su, Ying Animals Zebrafish Regeneration Heart Myocytes, Cardiac Transcriptome Zebrafish Proteins Single-Cell Analysis Myocardium Cell Differentiation Gene Expression Profiling Adult zebrafish robustly regenerate injured hearts through a complex orchestration of molecular and cellular activities. However, this remarkable process, which is largely non-existent in humans, remains incompletely understood. Here, we utilize integrated spatial transcriptomics (Stereo-seq) and single-cell RNA-sequencing (scRNA-seq) to generate a spatially-resolved molecular and cellular atlas of regenerating zebrafish heart across eight stages. We characterize the cascade of cardiomyocyte cell states responsible for producing regenerated myocardium and explore a potential role for tpm4a in cardiomyocyte re-differentiation. Moreover, we uncover the activation of ifrd1 and atp6ap2 genes as a unique feature of regenerative hearts. Lastly, we reconstruct a 4D "virtual regenerating heart" comprising 569,896 cells/spots derived from 36 scRNA-seq libraries and 224 Stereo-seq slices. Our comprehensive atlas serves as a valuable resource to the cardiovascular and regeneration scientific communities and their ongoing efforts to understand the molecular and cellular mechanisms underlying vertebrate heart regeneration. |
| title | An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart. |
| topic | Animals Zebrafish Regeneration Heart Myocytes, Cardiac Transcriptome Zebrafish Proteins Single-Cell Analysis Myocardium Cell Differentiation Gene Expression Profiling |
| url | https://pubmed.ncbi.nlm.nih.gov/40253397/ |