Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China.
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Antonie van Leeuwenhoek
2025
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| _version_ | 1868266214116032513 |
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| author | Wang, Ying Chen, Le-Bin OuYang, Yu-Ting Xing, Jia-Ying Zhang, Ke Chen, Nan-Feng She, Ting-Ting Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun |
| author_facet | Wang, Ying Chen, Le-Bin OuYang, Yu-Ting Xing, Jia-Ying Zhang, Ke Chen, Nan-Feng She, Ting-Ting Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun Wang, Ying Chen, Le-Bin OuYang, Yu-Ting Xing, Jia-Ying Zhang, Ke Chen, Nan-Feng She, Ting-Ting Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun |
| collection | PubMed - marine biology |
| contents | Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China. Wang, Ying Chen, Le-Bin OuYang, Yu-Ting Xing, Jia-Ying Zhang, Ke Chen, Nan-Feng She, Ting-Ting Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun China Soil Microbiology Phylogeny RNA, Ribosomal, 16S Fatty Acids Base Composition DNA, Bacterial Siderophores Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization A Gram-stain-positive, motile, and oval-shaped bacterium, designated as SYSU BS000539, was isolated from black soil in Heilongjiang Province, China. 16S rRNA gene sequencing revealed that the isolate shared the highest similarity to Trujillonella endophytica DSM 45413 (98.32%), and phylogenetic analysis confirmed that it belonged to the genus Trujillonella. The strain could grow at temperatures from 15 to 37 °C (with an optimum at 28 °C), in salt concentrations of 0-5% (w/v) (with an optimum at 0%), and at pH levels from 6.0 to 10.0 (with an optimum at 8.0). The dominant cellular fatty acids (> 5%) were iso-C, iso-C, and iso-C, while the principal respiratory quinones was menaquinone MK-9 (H) with menaquinone MK-8 as minor components. Polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol methylphosphonolipid, an unidentified aminolipid and two unidentified polar lipids. The G + C content of the genome was 74.97%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SYSU BS000539 and T. endophytica DSM 45413 were 84.10% and 27.60%, respectively, falling below species delineation thresholds. Combining phylogenetic, genotypic, chemotaxonomic, physiological and biochemical analyses, it is evident that SYSU BS000539 represented a novel species within the genus Trujillonella, for which the name Trujillonella humicola sp. nov. is proposed. The type strain of the proposed novel species is SYSU BS000539 (= MCCC 1K09330 = KCTC 59402). |
| format | Artículo científico |
| id | pubmed_40261431 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Antonie van Leeuwenhoek |
| record_format | pubmed |
| spellingShingle | Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China. Wang, Ying Chen, Le-Bin OuYang, Yu-Ting Xing, Jia-Ying Zhang, Ke Chen, Nan-Feng She, Ting-Ting Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun China Soil Microbiology Phylogeny RNA, Ribosomal, 16S Fatty Acids Base Composition DNA, Bacterial Siderophores Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China. Wang, Ying Chen, Le-Bin OuYang, Yu-Ting Xing, Jia-Ying Zhang, Ke Chen, Nan-Feng She, Ting-Ting Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun China Soil Microbiology Phylogeny RNA, Ribosomal, 16S Fatty Acids Base Composition DNA, Bacterial Siderophores Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization A Gram-stain-positive, motile, and oval-shaped bacterium, designated as SYSU BS000539, was isolated from black soil in Heilongjiang Province, China. 16S rRNA gene sequencing revealed that the isolate shared the highest similarity to Trujillonella endophytica DSM 45413 (98.32%), and phylogenetic analysis confirmed that it belonged to the genus Trujillonella. The strain could grow at temperatures from 15 to 37 °C (with an optimum at 28 °C), in salt concentrations of 0-5% (w/v) (with an optimum at 0%), and at pH levels from 6.0 to 10.0 (with an optimum at 8.0). The dominant cellular fatty acids (> 5%) were iso-C, iso-C, and iso-C, while the principal respiratory quinones was menaquinone MK-9 (H) with menaquinone MK-8 as minor components. Polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol methylphosphonolipid, an unidentified aminolipid and two unidentified polar lipids. The G + C content of the genome was 74.97%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SYSU BS000539 and T. endophytica DSM 45413 were 84.10% and 27.60%, respectively, falling below species delineation thresholds. Combining phylogenetic, genotypic, chemotaxonomic, physiological and biochemical analyses, it is evident that SYSU BS000539 represented a novel species within the genus Trujillonella, for which the name Trujillonella humicola sp. nov. is proposed. The type strain of the proposed novel species is SYSU BS000539 (= MCCC 1K09330 = KCTC 59402). |
| title | Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China. |
| topic | China Soil Microbiology Phylogeny RNA, Ribosomal, 16S Fatty Acids Base Composition DNA, Bacterial Siderophores Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization |
| url | https://pubmed.ncbi.nlm.nih.gov/40261431/ |