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| Auteurs principaux: | , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Marine drugs
2025
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40278294/ |
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| _version_ | 1868266211782950913 |
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| author | Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang |
| author_facet | Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang |
| collection | PubMed - marine biology |
| contents | Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of HN4-13. Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang Emodin Aspergillus High-Throughput Screening Assays Aquatic Organisms Mutation Mutagenesis Emodin is an anthraquinone compound known for its diverse biological activities, including anti-tumor and anti-inflammatory effects, making it highly applicable in the fields of biology and medicine. The production of emodin using microorganisms represents a sustainable and environmentally friendly approach. A marine-derived HN4-13 was found to produce emodin, but the yield was too low for industrial production. To develop a high-yielding emodin-producing strain, we established the high-through detection and screening methods of alkaline coloration and deep-well plant culture, enabling the effective selection of high-yielding strains. Following ARTP mutagenesis of the wild strain HN4-13, the resulting mutant strain, M1440, exhibited an increased emodin yield of 124.6 ± 4.95 mg/L. Furthermore, the production of the emodin was enhanced by the exogenous addition of metal ions Mn to the medium. Specifically, the addition of 3 mM Mn resulted in a 133.2% increase in emodin production, with the highest yield reaching 178.6 ± 7.80 mg/L. |
| format | Artículo científico |
| id | pubmed_40278294 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Marine drugs |
| record_format | pubmed |
| spellingShingle | Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of HN4-13. Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang Emodin Aspergillus High-Throughput Screening Assays Aquatic Organisms Mutation Mutagenesis Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of HN4-13. Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang Emodin Aspergillus High-Throughput Screening Assays Aquatic Organisms Mutation Mutagenesis Emodin is an anthraquinone compound known for its diverse biological activities, including anti-tumor and anti-inflammatory effects, making it highly applicable in the fields of biology and medicine. The production of emodin using microorganisms represents a sustainable and environmentally friendly approach. A marine-derived HN4-13 was found to produce emodin, but the yield was too low for industrial production. To develop a high-yielding emodin-producing strain, we established the high-through detection and screening methods of alkaline coloration and deep-well plant culture, enabling the effective selection of high-yielding strains. Following ARTP mutagenesis of the wild strain HN4-13, the resulting mutant strain, M1440, exhibited an increased emodin yield of 124.6 ± 4.95 mg/L. Furthermore, the production of the emodin was enhanced by the exogenous addition of metal ions Mn to the medium. Specifically, the addition of 3 mM Mn resulted in a 133.2% increase in emodin production, with the highest yield reaching 178.6 ± 7.80 mg/L. |
| title | Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of HN4-13. |
| topic | Emodin Aspergillus High-Throughput Screening Assays Aquatic Organisms Mutation Mutagenesis |
| url | https://pubmed.ncbi.nlm.nih.gov/40278294/ |