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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine drugs
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40278294/ |
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Table of Contents:
- Rational High-Throughput System for Screening Emodin High-Yielding Mutant from Marine Strains of HN4-13. Gong, Lizhi Li, Zixuan Xu, Meina Zhou, Yushan Zhang, Wenqing Zhao, Jian Xin, Xiujuan An, Faliang Emodin Aspergillus High-Throughput Screening Assays Aquatic Organisms Mutation Mutagenesis Emodin is an anthraquinone compound known for its diverse biological activities, including anti-tumor and anti-inflammatory effects, making it highly applicable in the fields of biology and medicine. The production of emodin using microorganisms represents a sustainable and environmentally friendly approach. A marine-derived HN4-13 was found to produce emodin, but the yield was too low for industrial production. To develop a high-yielding emodin-producing strain, we established the high-through detection and screening methods of alkaline coloration and deep-well plant culture, enabling the effective selection of high-yielding strains. Following ARTP mutagenesis of the wild strain HN4-13, the resulting mutant strain, M1440, exhibited an increased emodin yield of 124.6 ± 4.95 mg/L. Furthermore, the production of the emodin was enhanced by the exogenous addition of metal ions Mn to the medium. Specifically, the addition of 3 mM Mn resulted in a 133.2% increase in emodin production, with the highest yield reaching 178.6 ± 7.80 mg/L.