Production and Characterization of Poly-γ-Glutamic Acid by SDU.

Fuente: PubMed
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Autori principali: Guo, Guangyao, Wang, Han, Jia, Huiyuan, Ni, Haiping, Xu, Shouying, Zhang, Cuiying, Zhang, Youming, Wu, Yuxia, Tu, Qiang
Natura: Artículo científico
Lingua:en
Pubblicazione: Microorganisms 2025
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author Guo, Guangyao
Wang, Han
Jia, Huiyuan
Ni, Haiping
Xu, Shouying
Zhang, Cuiying
Zhang, Youming
Wu, Yuxia
Tu, Qiang
author_facet Guo, Guangyao
Wang, Han
Jia, Huiyuan
Ni, Haiping
Xu, Shouying
Zhang, Cuiying
Zhang, Youming
Wu, Yuxia
Tu, Qiang
Guo, Guangyao
Wang, Han
Jia, Huiyuan
Ni, Haiping
Xu, Shouying
Zhang, Cuiying
Zhang, Youming
Wu, Yuxia
Tu, Qiang
collection PubMed - marine biology
contents Production and Characterization of Poly-γ-Glutamic Acid by SDU. Guo, Guangyao Wang, Han Jia, Huiyuan Ni, Haiping Xu, Shouying Zhang, Cuiying Zhang, Youming Wu, Yuxia Tu, Qiang In this study, a SDU strain capable of producing poly-γ-glutamate (γ-PGA) was newly identified from the rhizosphere soil of Baimiao taro. The strain is a glutamate-independent strain and can produce polyglutamic acid in a culture medium completely free of glutamate. The hydrolyzed product of the polyglutamic acid produced is D-glutamic acid. The molecular weight of γ-PGA, estimated via the Mark-Houwink equation, was 1390 kDa. Furthermore, the molecular weight measured by Waters gel permeation chromatography with multi-angle laser light scattering (GPC-MALLS) was 1167 kDa. The production of γ-PGA and its antioxidant and tyrosine inhibition properties were investigated. The γ-PGA production reached 23.1 g/L, and the productivity was 0.77 g L h. Specifically, γ-PGA exhibited superoxide anion (·O) radical scavenging activity and tyrosinase inhibitory activity. This study introduces a promising strain and a highly efficient application method for γ-PGA, which can be broadly utilized in the pharmaceutical, food, and cosmetic industries.
format Artículo científico
id pubmed_40284754
institution PubMed
language en
publishDate 2025
publisher Microorganisms
record_format pubmed
spellingShingle Production and Characterization of Poly-γ-Glutamic Acid by SDU.
Guo, Guangyao
Wang, Han
Jia, Huiyuan
Ni, Haiping
Xu, Shouying
Zhang, Cuiying
Zhang, Youming
Wu, Yuxia
Tu, Qiang
Production and Characterization of Poly-γ-Glutamic Acid by SDU. Guo, Guangyao Wang, Han Jia, Huiyuan Ni, Haiping Xu, Shouying Zhang, Cuiying Zhang, Youming Wu, Yuxia Tu, Qiang In this study, a SDU strain capable of producing poly-γ-glutamate (γ-PGA) was newly identified from the rhizosphere soil of Baimiao taro. The strain is a glutamate-independent strain and can produce polyglutamic acid in a culture medium completely free of glutamate. The hydrolyzed product of the polyglutamic acid produced is D-glutamic acid. The molecular weight of γ-PGA, estimated via the Mark-Houwink equation, was 1390 kDa. Furthermore, the molecular weight measured by Waters gel permeation chromatography with multi-angle laser light scattering (GPC-MALLS) was 1167 kDa. The production of γ-PGA and its antioxidant and tyrosine inhibition properties were investigated. The γ-PGA production reached 23.1 g/L, and the productivity was 0.77 g L h. Specifically, γ-PGA exhibited superoxide anion (·O) radical scavenging activity and tyrosinase inhibitory activity. This study introduces a promising strain and a highly efficient application method for γ-PGA, which can be broadly utilized in the pharmaceutical, food, and cosmetic industries.
title Production and Characterization of Poly-γ-Glutamic Acid by SDU.
url https://pubmed.ncbi.nlm.nih.gov/40284754/