Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus.
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| Format: | Artículo científico |
| Sprache: | en |
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The Journal of experimental biology
2025
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| author | Lumor, Linda Bock, Christian Mark, Felix Christopher Ponsuksili, Siriluck Sokolova, Inna |
| author_facet | Lumor, Linda Bock, Christian Mark, Felix Christopher Ponsuksili, Siriluck Sokolova, Inna Lumor, Linda Bock, Christian Mark, Felix Christopher Ponsuksili, Siriluck Sokolova, Inna |
| collection | PubMed - marine biology |
| contents | Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus. Lumor, Linda Bock, Christian Mark, Felix Christopher Ponsuksili, Siriluck Sokolova, Inna Animals Oxidative Stress Mitochondria Energy Metabolism Pecten Oxygen Gills Oxidation-Reduction Reactive Oxygen Species Succinic Acid The king scallop (Pecten maximus) is a highly aerobic subtidal bivalve species vulnerable to fluctuations in oxygen availability. This study investigated the effects of short-term (15 min) and long-term (90 min) hypoxia-reoxygenation (H/R) stress on substrate-specific mitochondrial functions in the gill and digestive gland tissues of P. maximus, oxidizing substrates that engage mitochondrial Complex I (pyruvate, palmitate) and Complex II (succinate). Under normoxic conditions, scallop mitochondria preferentially oxidized pyruvate. H/R stress induced a significant decline in Complex I-driven ATP synthesis, increased proton leak and dysregulated fatty acid oxidation, indicating mitochondrial vulnerability to H/R stress. Following H/R, both tissues demonstrated a greater capacity for succinate oxidation than for Complex I substrates; however, long-term H/R exposure led to a reduction in respiratory coupling efficiency across all substrates. Notably, gill mitochondria exhibited more effective regulation of reactive oxygen species efflux and electron leak compared with digestive gland mitochondria under H/R stress. Despite these physiological changes, no evidence of oxidative damage was detected, suggesting the presence of a robust mitochondrial antioxidant defense. Collectively, these findings suggest that succinate oxidation plays an important role in stress recovery in P. maximus, providing insights into mitochondrial resilience and the management of oxidative stress during intermittent hypoxia. |
| format | Artículo científico |
| id | pubmed_40289682 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | The Journal of experimental biology |
| record_format | pubmed |
| spellingShingle | Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus. Lumor, Linda Bock, Christian Mark, Felix Christopher Ponsuksili, Siriluck Sokolova, Inna Animals Oxidative Stress Mitochondria Energy Metabolism Pecten Oxygen Gills Oxidation-Reduction Reactive Oxygen Species Succinic Acid Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus. Lumor, Linda Bock, Christian Mark, Felix Christopher Ponsuksili, Siriluck Sokolova, Inna Animals Oxidative Stress Mitochondria Energy Metabolism Pecten Oxygen Gills Oxidation-Reduction Reactive Oxygen Species Succinic Acid The king scallop (Pecten maximus) is a highly aerobic subtidal bivalve species vulnerable to fluctuations in oxygen availability. This study investigated the effects of short-term (15 min) and long-term (90 min) hypoxia-reoxygenation (H/R) stress on substrate-specific mitochondrial functions in the gill and digestive gland tissues of P. maximus, oxidizing substrates that engage mitochondrial Complex I (pyruvate, palmitate) and Complex II (succinate). Under normoxic conditions, scallop mitochondria preferentially oxidized pyruvate. H/R stress induced a significant decline in Complex I-driven ATP synthesis, increased proton leak and dysregulated fatty acid oxidation, indicating mitochondrial vulnerability to H/R stress. Following H/R, both tissues demonstrated a greater capacity for succinate oxidation than for Complex I substrates; however, long-term H/R exposure led to a reduction in respiratory coupling efficiency across all substrates. Notably, gill mitochondria exhibited more effective regulation of reactive oxygen species efflux and electron leak compared with digestive gland mitochondria under H/R stress. Despite these physiological changes, no evidence of oxidative damage was detected, suggesting the presence of a robust mitochondrial antioxidant defense. Collectively, these findings suggest that succinate oxidation plays an important role in stress recovery in P. maximus, providing insights into mitochondrial resilience and the management of oxidative stress during intermittent hypoxia. |
| title | Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus. |
| topic | Animals Oxidative Stress Mitochondria Energy Metabolism Pecten Oxygen Gills Oxidation-Reduction Reactive Oxygen Species Succinic Acid |
| url | https://pubmed.ncbi.nlm.nih.gov/40289682/ |