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| Natura: | Artículo científico |
| Lingua: | en |
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International journal of systematic and evolutionary microbiology
2025
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| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40293928/ |
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| _version_ | 1868266211752542209 |
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| author | Li, Qi Peng, Zi-Qi Liu, Wen-Ling Luo, Zhen-Hao Li, Shuai Li, Wen-Jun Dong, Lei |
| author_facet | Li, Qi Peng, Zi-Qi Liu, Wen-Ling Luo, Zhen-Hao Li, Shuai Li, Wen-Jun Dong, Lei Li, Qi Peng, Zi-Qi Liu, Wen-Ling Luo, Zhen-Hao Li, Shuai Li, Wen-Jun Dong, Lei |
| collection | PubMed - marine biology |
| contents | gen. nov., sp. nov., isolated from the Gurbantunggut Desert soil. Li, Qi Peng, Zi-Qi Liu, Wen-Ling Luo, Zhen-Hao Li, Shuai Li, Wen-Jun Dong, Lei Base Composition RNA, Ribosomal, 16S Phylogeny Soil Microbiology Fatty Acids China DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Desert Climate Sequence Analysis, DNA Phospholipids Genome, Bacterial Vitamin K 2 A Gram-stain-negative, aerobic, rod-shaped bacterial strain, SYSU D60014, was isolated from a sandy soil sample collected from the Gurbantunggut Desert in Xinjiang, PR China. Colonies of SYSU D60014 were pink-coloured, crystalline, irregular edge with a rough surface. Phylogenetic analyses based on both the 16S rRNA gene and whole-genome sequences assigned strain SYSU D60014 belonged to the family , formed a distinct lineage and showed 92.5-91.0% similarity to closely related strains. Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values between strain SYSU D60014 and members of its related species were 17.2-19.6%, 70.8-74.4% and 48.9-64.5%, respectively. The complete genome of strain SYSU D60014 was 5,100,926 bp with a DNA G+C content of 65.1%. Cells were oxidase-positive and catalase-negative. The strain could grow at 28-40 °C (optimum, 37 °C), pH 5.0-8.0 (optimum, pH 7.0), and in the presence of up to 3% NaCl (optimum, 1-1.5%, w/v) on R2A. The predominant menaquinone was Q-10. The main polar lipids were phosphatidylethanolamine, phosphatidylmethyl ethanolamine, diphosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, unidentified phospholipid, four unidentified polar lipids and two unidentified phosphoglycolipids. Major cellular fatty acids were C cyclo 8, summed feature 8 (C c and/or C c) and C. Based on the results obtained through genotypic and phenotypic analyses, we propose that strain SYSU D60014 represents a novel species and genus within the family , for which we propose the name gen. nov., sp. nov. (type strain SYSU D60014=NBRC 112951=CPCC 101030). |
| format | Artículo científico |
| id | pubmed_40293928 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of systematic and evolutionary microbiology |
| record_format | pubmed |
| spellingShingle | gen. nov., sp. nov., isolated from the Gurbantunggut Desert soil. Li, Qi Peng, Zi-Qi Liu, Wen-Ling Luo, Zhen-Hao Li, Shuai Li, Wen-Jun Dong, Lei Base Composition RNA, Ribosomal, 16S Phylogeny Soil Microbiology Fatty Acids China DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Desert Climate Sequence Analysis, DNA Phospholipids Genome, Bacterial Vitamin K 2 gen. nov., sp. nov., isolated from the Gurbantunggut Desert soil. Li, Qi Peng, Zi-Qi Liu, Wen-Ling Luo, Zhen-Hao Li, Shuai Li, Wen-Jun Dong, Lei Base Composition RNA, Ribosomal, 16S Phylogeny Soil Microbiology Fatty Acids China DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Desert Climate Sequence Analysis, DNA Phospholipids Genome, Bacterial Vitamin K 2 A Gram-stain-negative, aerobic, rod-shaped bacterial strain, SYSU D60014, was isolated from a sandy soil sample collected from the Gurbantunggut Desert in Xinjiang, PR China. Colonies of SYSU D60014 were pink-coloured, crystalline, irregular edge with a rough surface. Phylogenetic analyses based on both the 16S rRNA gene and whole-genome sequences assigned strain SYSU D60014 belonged to the family , formed a distinct lineage and showed 92.5-91.0% similarity to closely related strains. Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values between strain SYSU D60014 and members of its related species were 17.2-19.6%, 70.8-74.4% and 48.9-64.5%, respectively. The complete genome of strain SYSU D60014 was 5,100,926 bp with a DNA G+C content of 65.1%. Cells were oxidase-positive and catalase-negative. The strain could grow at 28-40 °C (optimum, 37 °C), pH 5.0-8.0 (optimum, pH 7.0), and in the presence of up to 3% NaCl (optimum, 1-1.5%, w/v) on R2A. The predominant menaquinone was Q-10. The main polar lipids were phosphatidylethanolamine, phosphatidylmethyl ethanolamine, diphosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, unidentified phospholipid, four unidentified polar lipids and two unidentified phosphoglycolipids. Major cellular fatty acids were C cyclo 8, summed feature 8 (C c and/or C c) and C. Based on the results obtained through genotypic and phenotypic analyses, we propose that strain SYSU D60014 represents a novel species and genus within the family , for which we propose the name gen. nov., sp. nov. (type strain SYSU D60014=NBRC 112951=CPCC 101030). |
| title | gen. nov., sp. nov., isolated from the Gurbantunggut Desert soil. |
| topic | Base Composition RNA, Ribosomal, 16S Phylogeny Soil Microbiology Fatty Acids China DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Desert Climate Sequence Analysis, DNA Phospholipids Genome, Bacterial Vitamin K 2 |
| url | https://pubmed.ncbi.nlm.nih.gov/40293928/ |