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| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine biotechnology (New York, N.Y.)
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40299219/ |
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Table of Contents:
- Sterilization of Channel Catfish (Ictalurus punctatus) via Overexpression of bax Gene Regulated by a Tet-off System in the Primordial Germ Cells. Ye, Zhi Elaswad, Ahmed Qin, Guyu Zhang, Dongdong Su, Baofeng Khalil, Karim Qin, Zhenkui Abass, Nermeen Y Cheng, Qi Odin, Ramjie Vo, Khoi Backenstose, Nathan Drescher, David Shang, Mei Li, Hanbo Zhang, Dan Bugg, William S Gosh, Kamal Dunham, Rex A Animals Ictaluridae Male Germ Cells Female Animals, Genetically Modified bcl-2-Associated X Protein Transgenes Electroporation Sex Ratio Sterilization, Reproductive Gonads Fish Proteins Transgenic technologies have been used for genetic improvement of catfish performance with notable success. However, these developments are useless from a commercialization standpoint without extremely efficient confinement. Transgenic sterilization has the potential to accomplish 100% reproductive confinement and avoid genetic exchange between transgenic or domestic genotypes and wild populations. The present study reports a novel sterilization method for channel catfish by overexpressing the pro-apoptosis gene bax, specifically in the primordial germ cells, to inhibit their proliferation. Three transgenic constructs were electroporated into channel catfish one-cell embryos, including Nanos-nanos, Nanos-dnd, and Dazl-vasa. Transgene integration, gonad development, and sex ratio were evaluated in P and F generations. The transgene was successfully integrated into the channel catfish genome, with variable rates depending on each construct. Mosaicism of transgene integration was widely evident in the P fish, as expected. All three constructs showed similar efficacy for sterilizing P male channel catfish, with approximately half of all males showing little to no gonadal development, resulting in a significantly lower (p