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Hauptverfasser: Ji, Jiajue, Kawai, Shuma, Takagi, Rina, Koiwai, Keiichiro, Kawano, Ryuji, Tagawa, Yoshiyuki
Format: Artículo científico
Sprache:en
Veröffentlicht: Lab on a chip 2025
Schlagworte:
Online-Zugang:https://pubmed.ncbi.nlm.nih.gov/40302460/
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author Ji, Jiajue
Kawai, Shuma
Takagi, Rina
Koiwai, Keiichiro
Kawano, Ryuji
Tagawa, Yoshiyuki
author_facet Ji, Jiajue
Kawai, Shuma
Takagi, Rina
Koiwai, Keiichiro
Kawano, Ryuji
Tagawa, Yoshiyuki
Ji, Jiajue
Kawai, Shuma
Takagi, Rina
Koiwai, Keiichiro
Kawano, Ryuji
Tagawa, Yoshiyuki
collection PubMed - marine biology
contents Generation of cell-sized liposomes using laser-induced microjets. Ji, Jiajue Kawai, Shuma Takagi, Rina Koiwai, Keiichiro Kawano, Ryuji Tagawa, Yoshiyuki Liposomes Lasers Lipid Bilayers Cell-sized liposomes are microcapsules composed of a lipid bilayer, with potential applications in membrane science and synthetic biology. In this study, we present a novel method that employs high-speed laser-induced microjets to penetrate a lipid-carrying oil phase, thereby generating cell-sized liposomes. By simply triggering the microjets, we can reliably and repeatedly generate cell-sized liposomes, thereby enabling on-demand liposome production. We employed a high-speed camera to capture and analyze the penetration behavior of microjets. Additionally, we confirmed the unilamellarity of the liposomes using melittin, confirming their suitability for various biochemical applications. Furthermore, we conducted a numerical analysis to investigate potential factors influencing liposome formation in detail. These findings hold promise for advancing on-demand liposome production and contributing to the development of biochemical research.
format Artículo científico
id pubmed_40302460
institution PubMed
language en
publishDate 2025
publisher Lab on a chip
record_format pubmed
spellingShingle Generation of cell-sized liposomes using laser-induced microjets.
Ji, Jiajue
Kawai, Shuma
Takagi, Rina
Koiwai, Keiichiro
Kawano, Ryuji
Tagawa, Yoshiyuki
Liposomes
Lasers
Lipid Bilayers
Generation of cell-sized liposomes using laser-induced microjets. Ji, Jiajue Kawai, Shuma Takagi, Rina Koiwai, Keiichiro Kawano, Ryuji Tagawa, Yoshiyuki Liposomes Lasers Lipid Bilayers Cell-sized liposomes are microcapsules composed of a lipid bilayer, with potential applications in membrane science and synthetic biology. In this study, we present a novel method that employs high-speed laser-induced microjets to penetrate a lipid-carrying oil phase, thereby generating cell-sized liposomes. By simply triggering the microjets, we can reliably and repeatedly generate cell-sized liposomes, thereby enabling on-demand liposome production. We employed a high-speed camera to capture and analyze the penetration behavior of microjets. Additionally, we confirmed the unilamellarity of the liposomes using melittin, confirming their suitability for various biochemical applications. Furthermore, we conducted a numerical analysis to investigate potential factors influencing liposome formation in detail. These findings hold promise for advancing on-demand liposome production and contributing to the development of biochemical research.
title Generation of cell-sized liposomes using laser-induced microjets.
topic Liposomes
Lasers
Lipid Bilayers
url https://pubmed.ncbi.nlm.nih.gov/40302460/